{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,2]],"date-time":"2025-03-02T05:10:16Z","timestamp":1740892216431,"version":"3.38.0"},"reference-count":54,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p> Previous studies in our laboratory showed a dose-dependent and hormone-specific increase in CD154 expression in T cells from females with systemic lupus erythematosus (SLE). This present study investigates if the estrogen-dependent increase in CD154 expression is due to stabilization of the messenger RNA. T cells from female SLE patients and controls were cultured for 18 h in serum-free medium without and with estradiol 17-\u03b2 (10<jats:sup>7<\/jats:sup> M). T cells were either unstimulated (resting) or were activated by further culture on anti-CD3 coated plates. Actinomycin D (25 \u03c3g\/mL) was added to parallel cultures to inhibit new messenger RNA synthesis. CD154 messenger RNA stability was assessed by reverse-transcription polymerase chain amplification. Resting SLE ( n = 10, P = 0.88) and normal ( n = 7, P = 0.65) T cells showed no significant differences in message stability in response to estradiol. CD154 messenger RNA was also not significantly stabilized in activated SLE ( n = 10, P = 0.15) or activated normal ( n = 6, P = 0.077) T cells in response to estradiol. These findings indicate that the estrogen-dependent increase in CD154 in SLE T cells is not due to stability of the mRNA. These data are consistent with the postulate that estradiol stimulates CD154 transcription in SLE T cells. <\/jats:p>","DOI":"10.1177\/0961203306071314","type":"journal-article","created":{"date-parts":[[2006,12,5]],"date-time":"2006-12-05T10:28:21Z","timestamp":1165314501000},"page":"852-857","source":"Crossref","is-referenced-by-count":4,"title":["Estrogen does not regulate CD154 mRNA stability in systemic lupus                 erythematosus T cells"],"prefix":"10.1177","volume":"15","author":[{"given":"X","family":"Li","sequence":"first","affiliation":[{"name":"Department of Biology, Pittsburg State University, Pittsburg, Kansas,                         USA, Department of Dermatology and Rheumatology, Kunming Medical College,                         Kunming, People\u2019s Republic of China"}]},{"given":"V","family":"Rider","sequence":"additional","affiliation":[{"name":"Department of Biology, Pittsburg State University, Pittsburg, Kansas,                         USA,"}]},{"given":"B 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