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Our aim was to determine whether human umbilical vein endothelial cells (HUVEC) isolated from healthy newborns to women with inactive SLE show inflammation-related abnormalities that might lead to an early development of SLE in the offsprings. HUVEC isolated from six women with inactive SLE were stimulated with 2.5\u00a0ng\/mL of TNF-\u03b1 and\/or physiological and pharmacological doses of 17-\u03b2 estradiol (E2). Then the expression of VCAM-1, ICAM-1, E-selectin, toll-like receptor-9 (TLR-9), heat shock protein 70 (HSP70) and HSP90 were measured. The concentrations of IL-6, IL-8, and IL-10 were also determined in maternal serum and in TNF-\u03b1 stimulated and non-stimulated HUVEC culture supernatant. HUVEC from children with no family history of autoimmune disease served as controls. Our results showed that in HUVEC from SLE+ mothers, a constitutively low expression of adhesion molecules was enhanced by TNF-\u03b1 treatment. The E2 (1\u00a0ng\/mL) increased the expression of adhesion molecules but had no effect upon TNF-\u03b1-treated cells. IL-6 was constitutively higher in SLE+ HUVEC, whereas IL-8 was lower; E2 treatment diminished the latter. The E2 had no effect upon IL-6 and IL-8 secretions in TNF-\u03b1-treated cells. SLE+ HUVEC showed a disordered cytoskeleton and overexpressed HSP70, HSP90, and TLR-9. Our results indicate that endothelial cells of newborns to SLE+ mothers are in a proinflammatory condition which can be upregulated by estrogens.<\/jats:p>","DOI":"10.1177\/0961203308093827","type":"journal-article","created":{"date-parts":[[2008,11,24]],"date-time":"2008-11-24T11:59:20Z","timestamp":1227527960000},"page":"1086-1095","source":"Crossref","is-referenced-by-count":12,"title":["The altered expression of inflammation-related molecules\u00a0and\u00a0secretion of IL-6 and IL-8 by HUVEC from\u00a0newborns with\u00a0maternal inactive systemic lupus erythematosus is modified by estrogens"],"prefix":"10.1177","volume":"17","author":[{"given":"E","family":"Rodriguez","sequence":"first","affiliation":[{"name":"Depto. Biolog\u00eda Celular, Instituto Nacional de Cardiolog\u00eda \u201cIgnacio Ch\u00e1vez\u201d, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Guevara","sequence":"additional","affiliation":[{"name":"Depto. 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