{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:02:09Z","timestamp":1777928529794,"version":"3.51.4"},"reference-count":52,"publisher":"SAGE Publications","issue":"4-5","license":[{"start":{"date-parts":[[2015,3,23]],"date-time":"2015-03-23T00:00:00Z","timestamp":1427068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>Systemic lupus erythematosus (SLE) is an autoimmune disease that predominantly affects fertile women, suggesting sex hormones are involved in disease pathogenesis. B lymphocyte stimulator (BLyS) has been found to be elevated in SLE patients and to drive a lupus-like syndrome in transgenic mice.<\/jats:p>\n                  <jats:p>Our aim was to evaluate the effects of estrogen administration on BLyS and nephritogenic anti-C1q and anti-dsDNA antibodies in lupus-prone NZB\/WF1 mice.<\/jats:p>\n                  <jats:p>We implanted pellets releasing 17-\u03b2-estradiol (18.8\u2009\u00b5g\/day) on the back side the ear of 10 NZB\/WF1 mice (group 1), and compared them with 10 mice intraperitoneally injected with PBS 200\u2009\u03bcl twice a week (group 2), as controls. We evaluated BLyS, anti-dsDNA and anti-C1q serum levels starting one week after pellet implantation. We also analyzed time to proteinuria onset, proteinuria-free survival and overall survival. Kidneys, spleen, liver and lungs were harvested for histological analysis. Mice were bred until natural death.<\/jats:p>\n                  <jats:p>\n                    BLyS serum levels were higher in group 1 than in group 2 mice at each evaluation. Group 1 mice developed nephritogenic antibodies and proteinuria significantly earlier and at higher levels than controls. Direct correlation between BLyS and anti-C1q ( R\n                    <jats:sup>2\u2009<\/jats:sup>\n                    =\u20090.6962, p\u2009&lt;\u20090.0001) or anti-dsDNA ( R\n                    <jats:sup>2\u2009<\/jats:sup>\n                    =\u20090.5953, p\u2009&lt;\u20090.0001), and between anti-C1q and anti-dsDNA autoantibodies ( R\n                    <jats:sup>2\u2009<\/jats:sup>\n                    =\u20090.5615, p\u2009&lt;\u20090.0001) were found. Proteinuria-free and global survival rates were significantly lower in group 1 than in controls. Histological analyses showed more severe abnormalities in group 1 mice.\n                  <\/jats:p>\n                  <jats:p>Estrogen administration is associated with increased levels of BLyS as well as of anti-C1q and anti-dsDNA antibodies, leading to accelerated glomerulonephritis and disease progression in NZB\/WF1 mice.<\/jats:p>","DOI":"10.1177\/0961203314559636","type":"journal-article","created":{"date-parts":[[2015,3,23]],"date-time":"2015-03-23T07:20:52Z","timestamp":1427095252000},"page":"382-391","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["17-\u03b2-estradiol affects BLyS serum levels and the nephritogenic autoantibody network accelerating glomerulonephritis in NZB\/WF1 mice"],"prefix":"10.1177","volume":"24","author":[{"given":"N","family":"Bassi","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Department of Medicine-DIMED"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Luisetto","sequence":"additional","affiliation":[{"name":"Division of Surgical, Oncological, and Gastroenterological Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Ghirardello","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine-DIMED"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Gatto","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine-DIMED"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Valente","sequence":"additional","affiliation":[{"name":"Department of Cardiac, Thoracic, and Vascular Sciences, University of Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Della Barbera","sequence":"additional","affiliation":[{"name":"Department of Cardiac, Thoracic, and Vascular Sciences, University of Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Nalotto","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine-DIMED"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Punzi","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine-DIMED"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Doria","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of 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