{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:02:39Z","timestamp":1777928559652,"version":"3.51.4"},"reference-count":36,"publisher":"SAGE Publications","issue":"13","license":[{"start":{"date-parts":[[2015,7,22]],"date-time":"2015-07-22T00:00:00Z","timestamp":1437523200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>Cutaneous lupus erythematosus (CLE) is an inflammatory autoimmune skin disease in which abnormal photosensitivity is an important pathogenetic factor but is difficult to predict, creating a challenge in determining treatment efficacy. Although photosensitivity in CLE patients may change over time, photoprovocation testing with ultraviolet (UV) A and UVB irradiation can be a helpful tool to explore differences between responders and nonresponders during photoprovocation. To identify biomarkers that could substitute for the clinical endpoint lesion development, we performed a global peptidomics profiling analysis of CLE subjects in a controlled photoprovocation study. Plasma and skin biopsy samples were collected before and after UV-irradiation from 13 healthy volunteers and 47 CLE subjects. Twenty-two of the 47 CLE subjects developed skin lesions. The samples were analyzed using a label-free quantitative peptidomics workflow combined with univariate and multivariate statistical analyses. The primary finding was identification of a specific plasma peptide signature separating responders versus nonresponders at baseline. The peptide signature consisted of beta 2-microglobulin (B2MG), human beta-defensin-1, and peptides derived from CD99, polymeric immunoglobulin receptor, and immunoglobulin kappa light chains. In skin, elevated B2MG levels correlated with lesion formation. Our results show that the peptidome is a rich source of potential biomarkers for predicting photosensitivity in CLE.<\/jats:p>","DOI":"10.1177\/0961203315596077","type":"journal-article","created":{"date-parts":[[2015,7,23]],"date-time":"2015-07-23T20:10:58Z","timestamp":1437682258000},"page":"1406-1420","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["A multicenter photoprovocation study to identify potential biomarkers by global peptide profiling in cutaneous lupus erythematosus"],"prefix":"10.1177","volume":"24","author":[{"given":"C","family":"Calderon","sequence":"first","affiliation":[{"name":"Compound Development, Janssen Research & Development, LLC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H-D","family":"Zucht","sequence":"additional","affiliation":[{"name":"Formerly of Digilab BioVisioN GmbH, Germany; currently of Protagen AG, Dortmund, 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