{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:07:50Z","timestamp":1777928870620,"version":"3.51.4"},"reference-count":41,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81373185"],"award-info":[{"award-number":["81373185"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Lupus B cells not only produce autoantibodies against nuclear antigens but also provide co-stimulation to T cells. However, there is still a lack of comprehensive understanding of the mechanism underlying lupus B cell hyperactivation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study focuses on the detection of B cell activation status, analysis of early BCR signaling response, DNA sequencing, and quantity determination of BCR signaling regulators in murine lupus models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our result showed that there is a B cell hyperactivation with a significant elevation of B cell activation markers, and a BCR signaling hyperactivity with an abnormal increase of phosphorylated BCR signaling molecules and cytoplasmic calcium in the early response to BCR crosslinking in B6.Sle1\/2\/3 lupus mouse. Whole exome sequencing identified a multiple point mutation in the exon of many BCR signaling regulators in common murine lupus models, MRL\/lpr, NZM2410, BXSB, NZB, and NZW strains. cNDA sequencing confirmed Fc\u03b3R2b, Ly9, Pirb, Siglecg, and CD22 BCR signaling regulator variants in B6.Sle1\/2\/3 lupus mouse, but surface protein expression of these regulators on B cells showed an abnormal increase.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings support that these BCR signaling regulator variants are potential causative genes of B cell hyperactivation in murine lupus models through their possible functional reduction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09612033211018264","type":"journal-article","created":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T03:39:03Z","timestamp":1621309143000},"page":"1438-1448","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Potential genetic basis of B cell hyperactivation in murine lupus models"],"prefix":"10.1177","volume":"30","author":[{"given":"JY","family":"Ju","sequence":"first","affiliation":[{"name":"Department of Immunology, Weifang Medical University, Weifang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7128-5811","authenticated-orcid":false,"given":"ZW","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Immunology, Weifang Medical University, Weifang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,5,18]]},"reference":[{"key":"bibr1-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/271694"},{"key":"bibr2-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(94)90100-7"},{"key":"bibr3-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.12.6670"},{"key":"bibr4-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2006.03.009"},{"key":"bibr5-09612033211018264","doi-asserted-by":"crossref","first-page":"4222","DOI":"10.4049\/jimmunol.138.12.4222","volume":"138","author":"Cerny A","year":"1987","journal-title":"J Immunol"},{"key":"bibr6-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.4.1295"},{"key":"bibr7-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1172\/JCI30398"},{"key":"bibr8-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00228-X"},{"key":"bibr9-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1126\/science.1125893"},{"key":"bibr10-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2004.10.009"},{"key":"bibr11-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.4.1787"},{"key":"bibr12-09612033211018264","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.2.936"},{"key":"bibr13-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6364426"},{"key":"bibr14-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2013.28"},{"key":"bibr15-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.10.1721"},{"key":"bibr16-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa0835"},{"key":"bibr17-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.24"},{"key":"bibr18-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2003.01592.x"},{"key":"bibr19-09612033211018264","doi-asserted-by":"publisher","DOI":"10.2174\/187152612800564455"},{"key":"bibr20-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2013.11.007"},{"key":"bibr21-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000000169"},{"key":"bibr22-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-015-1252-8"},{"key":"bibr23-09612033211018264","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00461"},{"key":"bibr24-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12381"},{"key":"bibr25-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.2MR0316-117RR"},{"key":"bibr26-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2013.114"},{"key":"bibr27-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.186"},{"key":"bibr28-09612033211018264","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.3.1581"},{"key":"bibr29-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-12-261461"},{"key":"bibr30-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.08.042"},{"key":"bibr31-09612033211018264","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200940"},{"key":"bibr32-09612033211018264","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1501963"},{"key":"bibr33-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.63"},{"key":"bibr34-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1038\/379346a0"},{"key":"bibr35-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.1.179"},{"key":"bibr36-09612033211018264","first-page":"221","volume":"4","author":"De SJ","year":"2013","journal-title":"Front Immunol"},{"key":"bibr37-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/275302"},{"key":"bibr38-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12344"},{"key":"bibr39-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.54"},{"key":"bibr40-09612033211018264","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2017.8"},{"key":"bibr41-09612033211018264","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00637"}],"container-title":["Lupus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09612033211018264","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09612033211018264","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09612033211018264","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T23:23:50Z","timestamp":1777677830000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09612033211018264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,18]]},"references-count":41,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1177\/09612033211018264"],"URL":"https:\/\/doi.org\/10.1177\/09612033211018264","relation":{},"ISSN":["0961-2033","1477-0962"],"issn-type":[{"value":"0961-2033","type":"print"},{"value":"1477-0962","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,18]]}}}