{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T05:35:06Z","timestamp":1781760906311,"version":"3.54.5"},"reference-count":44,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["No.16ZR1404900"],"award-info":[{"award-number":["No.16ZR1404900"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No.81703122"],"award-info":[{"award-number":["No.81703122"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No.81773324"],"award-info":[{"award-number":["No.81773324"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Discoid lupus erythematosus (DLE) is the most common category of chronic cutaneous lupus erythematosus, where the pathological process is proved to be closely associated with immunity. This bioinformatic analysis sought to identify key biomarkers and to perform immune infiltration analysis in the skin biopsy samples of DLE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>GSE120809, GSE100093, GSE72535, GSE81071 were used as the data source of gene expression profiles, altogether containing 79 DLE samples and 47 normal controls (NC). Limma package was applied to identify differentially expressed genes (DEGs) and additional Gene Ontology (GO) together with The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were done. Protein-protein interaction network (PPI) was constructed using STRING and Cytoscape. Hub genes were selected by CytoHubba. Finally, immune filtration analysis was finished by the CIBERSORT algorithm, and comparisons between the two groups were accomplished.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 391 DEGs were identified, which were composed of 57 up-regulated genes and 334 down-regulated genes. GO and KEGG enrichment analyses revealed that DEGs were closely related with different steps in the immune response. Top 10 hub genes included GBP2, HLA-F, IFIT2, RSAD2, ISG15, IFIT1, IFIT3, MX1, XAF1 and IFI6. Immune filtration analysis from CIBERSORT had found that compared with NC, DLE samples had higher percentages of CD8+ T cells, T cells CD4 memory activated, T cells gamma delta, macrophages M1 and lower percentages of T cells regulatory, macrophages M2, dendritic cells resting, mast cells resting, mast cells activated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This bioinformatic study selected key biomarkers from the contrast between DLE and NC skin samples and is the first research to analyze immune cell filtration in DLE.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0961203321992434","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T10:46:29Z","timestamp":1612953989000},"page":"807-817","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["Bioinformatic analysis of key biomarkers and immune filtration of skin biopsy in discoid lupus erythematosus"],"prefix":"10.1177","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6752-4827","authenticated-orcid":false,"given":"Mengmeng","family":"Xiang","sequence":"first","affiliation":[{"name":"Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China"},{"name":"Cutaneous Biology Research Center & Melanoma Program MGH Cancer Center, Harvard Medical School\/Massachusetts General Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhua","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China"},{"name":"Department of Dermatology, Shanghai Institute of Dermatology, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"bibr1-0961203321992434","doi-asserted-by":"publisher","DOI":"10.1002\/acr.22502"},{"key":"bibr2-0961203321992434","doi-asserted-by":"crossref","unstructured":"Garelli CJ, Refat MA, Nanaware PP, Ramirez-Ortiz ZG, Rashighi M, Richmond JM. Current Insights in Cutaneous Lupus Erythematosus Immunopathogenesis.\n                      Front Immunol\n                      2020; 11: 1353.","DOI":"10.3389\/fimmu.2020.01353"},{"key":"bibr3-0961203321992434","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-019-0272-0"},{"key":"bibr4-0961203321992434","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.02.030"},{"key":"bibr5-0961203321992434","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2018.02.034"},{"key":"bibr6-0961203321992434","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2018.04.002"},{"key":"bibr7-0961203321992434","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2018.02.011"},{"key":"bibr8-0961203321992434","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0000000000000383"},{"key":"bibr9-0961203321992434","doi-asserted-by":"crossref","first-page":"9","DOI":"10.3389\/fimmu.2019.02868","volume":"10","author":"Cuitino L","year":"2019","journal-title":"Front Immunol"},{"key":"bibr10-0961203321992434","doi-asserted-by":"crossref","unstructured":"Parisis D, Bernier C, Chasset F, Arnaud L. 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Type I Interferons in the Pathogenesis and Treatment of Autoimmune Diseases.\n                      Clinic Rev Allerg Immunol\n                      . Epub ahead of print 17 June 2020. DOI: 10.1007\/s12016-020-08798-2.","DOI":"10.1007\/s12016-020-08798-2"},{"key":"bibr32-0961203321992434","first-page":"1662","volume":"56","author":"Psarras A","year":"2017","journal-title":"Rheumatology (Oxford)"},{"key":"bibr33-0961203321992434","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-018-0010-6"},{"key":"bibr34-0961203321992434","doi-asserted-by":"crossref","unstructured":"Sonya JW, Shannon NE, Jonathan T, Tyson M, Michelle K. 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