{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T16:06:40Z","timestamp":1783354000828,"version":"3.54.6"},"reference-count":38,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T00:00:00Z","timestamp":1678233600000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.sagepub.com\/licence-information-for-chorus"}],"funder":[{"name":"Veterans Affairs Merit Review","award":["VA-ORD 5 I01 BX000706"],"award-info":[{"award-number":["VA-ORD 5 I01 BX000706"]}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["LRP-CALR190107"],"award-info":[{"award-number":["LRP-CALR190107"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Health","award":["R01 AR071653"],"award-info":[{"award-number":["R01 AR071653"]}]},{"name":"National Institute of Health","award":["RO1 AR076766"],"award-info":[{"award-number":["RO1 AR076766"]}]},{"DOI":"10.13039\/100012051","name":"Lupus Research Alliance","doi-asserted-by":"publisher","award":["K24 AR002207"],"award-info":[{"award-number":["K24 AR002207"]}],"id":[{"id":"10.13039\/100012051","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Antimalarials are first-line systemic therapy for cutaneous lupus erythematosus (CLE). While some patients unresponsive to hydroxychloroquine (HCQ) alone benefit from the addition of quinacrine (QC), a subset of patients is refractory to both antimalarials.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We classified CLE patients as HCQ-responders, HCQ+QC-responders, or HCQ+QC-nonresponders to compare immune profiles. Immunohistochemistry, immunofluorescence, and qRT-PCR were used to characterize inflammatory cells and cytokines in lesional skin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Immunohistochemistry showed that CD69\n                      <jats:sup>+<\/jats:sup>\n                      T cells were higher in HCQ+QC-nonresponders compared to HCQ- and HCQ+QC-responders ( p &lt; 0.05). Immunofluorescence further identified these cells as CD69+CCR7+ circulating activated T cells. Myeloid dendritic cells were significantly higher in HCQ+QC-responders compared to both HCQ-responders and HCQ+QC-nonresponders. Plasmacytoid dendritic cells were significantly increased in HCQ-responders compared to HCQ- and HCQ+QC-nonresponders. No differences were found in the number of autoreactive T cells, MAC387+ cells, and neutrophils among the groups. CLASI scores of the HCQ+QC-nonresponder group positively correlated with CD69+CCR7+ circulating activated T cells (r = 0.6335, p &lt; 0.05) and MAC387+ cells (r = 0.5726, p &lt; 0.05). IL-17 protein expression was higher in HCQ+QC-responders compared to HCQ-responders or HCQ+QC-nonresponders, while IL-22 protein expression did not differ. mRNA expression demonstrated increased STAT3 expression in a subset of HCQ+QC-nonresponders.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>An increased number of CD69+CCR7+ circulating activated T cells and a strong correlation with CLASI scores in the HCQ+QC-nonresponders suggest these cells are involved in antimalarial-refractory skin disease. STAT3 is also increased in HCQ+QC-nonresponders and may also be a potential target for antimalarial-refractory skin disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09612033221084093","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T08:49:20Z","timestamp":1646729360000},"page":"472-481","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Increased CD69+CCR7+ circulating activated T cells and STAT3 expression in cutaneous lupus erythematosus patients recalcitrant to antimalarials"],"prefix":"10.1177","volume":"31","author":[{"given":"Majid","family":"Zeidi","sequence":"first","affiliation":[{"name":"Corporal Michael J Crescenz VAMC, Philadelphia, PA, USA"},{"name":"Department of Dermatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen L","family":"Chen","sequence":"additional","affiliation":[{"name":"Corporal Michael J Crescenz VAMC, Philadelphia, PA, USA"},{"name":"Department of Dermatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4737-8740","authenticated-orcid":false,"given":"Jay","family":"Patel","sequence":"additional","affiliation":[{"name":"Corporal Michael J Crescenz VAMC, Philadelphia, PA, USA"},{"name":"Department of Dermatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krisha","family":"Desai","sequence":"additional","affiliation":[{"name":"Corporal Michael J Crescenz VAMC, Philadelphia, PA, USA"},{"name":"Department of Dermatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 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