{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:47:08Z","timestamp":1792788428837,"version":"4.5.0"},"reference-count":17,"publisher":"SAGE Publications","issue":"13","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Interferon-\u03b3 inducible protein-10 (IP-10) is a promising biomarker in systemic lupus erythematosus (SLE). The optimal quantification platform has not yet been identified. We compared the performance of bead-based multiplex assay (Luminex) and high-sensitivity enzyme-linked immunosorbent assay (hs-ELISA) for profiling serum IP-10 as a biomarker of lupus activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A cross-sectional study was conducted on outpatients with SLE. Serum IP-10 was measured simultaneously on Luminex and hs-ELISA, and correlation between platforms was assessed. Additionally, IP-10 levels were tested against disease activity and organ involvement.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>One-hundred and forty-one patients (88% women; 38 years old) were studied. Median IP-10 levels were 100.9 (125.2) pg\/mL by Luminex and 156.5 (191.7) pg\/mL by hs-ELISA. Correlation analysis showed Spearman\u2019s \u03c1 = 0.621 ( p &lt; 0.0001) between Luminex and hs-ELISA. Quantification of IP-10 by Luminex showed a significant correlation (\u03c1 = 0.198; p = 0.021) with disease activity, while this was not observed (\u03c1 = 0.036; p = 0.683) when measured using hs-ELISA. Serum IP-10 levels were lower in quiescent patients than in those with active disease (70.8 [68.4] versus 114.3 [123.9] pg\/mL; p = 0.024), with an AUC-ROC = 0.62 ( p = 0.029), sensitivity = 47.9%, specificity = 77.5%, and positive likelihood ratio = 2.1. Patients with active arthritis had higher IP-10 levels than non-arthritis patients (158.1 [505.4] versus 94.1 [114.0] pg\/mL; p = 0.008), with an AUC-ROC = 0.73 ( p = 0.0009), sensitivity = 72.7%, specificity = 66.4%, and positive likelihood ratio = 2.1. No other type of organ involvement was identified by serum IP-10.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Luminex performs better than hs-ELISA as a quantification platform for IP-10 as it correlates with disease activity and identifies active arthritis in SLE.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09612033221122978","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T09:18:59Z","timestamp":1661851139000},"page":"1660-1665","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Performance analysis of Luminex and ELISA to profile serum IP-10 as a biomarker in systemic lupus erythematosus"],"prefix":"10.1177","volume":"31","author":[{"given":"Fernanda","family":"Espinosa-Bautista","sequence":"first","affiliation":[{"name":"Immunology Department, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Mexico City, Mexico"},{"name":"School of Medicine, Facultad de Estudios Superiores Iztacala, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dania","family":"Coronel","sequence":"additional","affiliation":[{"name":"Immunology Department, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Mexico City, Mexico"},{"name":"School of Medicine, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla, Puebla, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Varna","family":"Ramos-Rosillo","sequence":"additional","affiliation":[{"name":"Immunology Department, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Mexico City, Mexico"},{"name":"School of Medicine, Universidad Aut\u00f3noma Metropolitana\u2013Xochimilco, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6258-5732","authenticated-orcid":false,"given":"Luis M","family":"Amezcua-Guerra","sequence":"additional","affiliation":[{"name":"Immunology Department, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Mexico City, Mexico"},{"name":"Health Care Department, Universidad Aut\u00f3noma Metropolitana\u2013Xochimilco, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"bibr1-09612033221122978","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20194954"},{"key":"bibr2-09612033221122978","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2019.102374"},{"key":"bibr3-09612033221122978","doi-asserted-by":"publisher","DOI":"10.21037\/atm-20-5232"},{"key":"bibr4-09612033221122978","doi-asserted-by":"publisher","DOI":"10.1177\/0961203320909429"},{"key":"bibr5-09612033221122978","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2021.155640"},{"key":"bibr6-09612033221122978","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2008.12.002"},{"key":"bibr7-09612033221122978","doi-asserted-by":"publisher","DOI":"10.1002\/art.24803"},{"key":"bibr8-09612033221122978","first-page":"4067","volume":"18","author":"Zhang R","year":"2019","journal-title":"Exp Ther 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