{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:38:49Z","timestamp":1790019529323,"version":"4.0.1"},"reference-count":62,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["1401-2-96-59269"],"award-info":[{"award-number":["1401-2-96-59269"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Systemic Lupus Erythematosus (SLE), a complex autoimmune disorder characterized by altered immune regulation, particularly involving B cells, which exhibit increased survival and developmental dysregulation. This study aimed to investigate the expression of IRF4, IRF8, SP1, and PU.1 in B cells of SLE patients, as these factors are known to play critical roles in the development, function, and differentiation of B cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      B cells were isolated, cultured, and activated using anti-IgM. The mRNA expression of\n                      <jats:italic>IRF4<\/jats:italic>\n                      ,\n                      <jats:italic>IRF8<\/jats:italic>\n                      ,\n                      <jats:italic>SP1<\/jats:italic>\n                      , and\n                      <jats:italic>PU<\/jats:italic>\n                      .\n                      <jats:italic>1<\/jats:italic>\n                      was assessed using reverse transcription polymerase chain reaction (RT-PCR) at baseline and after B cell activation. Correlations between transcription factor expression and clinical parameters were analyzed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Upon B cell activation,\n                      <jats:italic>IRF4<\/jats:italic>\n                      expression increased significantly in SLE patients, unlike at baseline, where no changes were observed between groups.\n                      <jats:italic>IRF8<\/jats:italic>\n                      expression was significantly raised in active SLE and increased upon activation.\n                      <jats:italic>SP1<\/jats:italic>\n                      expression remained stable across all groups and conditions.\n                      <jats:italic>PU<\/jats:italic>\n                      .\n                      <jats:italic>1<\/jats:italic>\n                      gene expression was higher in active SLE at baseline and increased further upon B cell activation. Positive correlations were found between\n                      <jats:italic>IRF4<\/jats:italic>\n                      and\n                      <jats:italic>IRF8<\/jats:italic>\n                      , as well as between\n                      <jats:italic>PU<\/jats:italic>\n                      .\n                      <jats:italic>1<\/jats:italic>\n                      and\n                      <jats:italic>SP1<\/jats:italic>\n                      .\n                      <jats:italic>PU<\/jats:italic>\n                      .\n                      <jats:italic>1<\/jats:italic>\n                      expression correlated with SLE disease activity indices.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>IRF4<\/jats:italic>\n                      ,\n                      <jats:italic>IRF8<\/jats:italic>\n                      , and\n                      <jats:italic>PU<\/jats:italic>\n                      .\n                      <jats:italic>1<\/jats:italic>\n                      expression in B cells is altered in SLE.\n                      <jats:italic>PU<\/jats:italic>\n                      .\n                      <jats:italic>1<\/jats:italic>\n                      shows a positive correlation with SLEDAI and anti-dsDNA titers. These findings highlight their potential roles in the pathogenesis of SLE in B cells.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09612033251366400","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T10:20:04Z","timestamp":1754389204000},"page":"1135-1146","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Overexpression of IRF8 and Pu.1 in B cells of systemic lupus erythematosus patients"],"prefix":"10.1177","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9633-4678","authenticated-orcid":false,"given":"Amin","family":"Azizan","sequence":"first","affiliation":[{"name":"Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran"},{"name":"Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran"},{"name":"Research Center for Chronic Inflammatory Diseases, 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