{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T01:51:24Z","timestamp":1788054684716,"version":"build-2784847793"},"reference-count":33,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T00:00:00Z","timestamp":1755907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","award":["2024YFFK0222"],"award-info":[{"award-number":["2024YFFK0222"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2022YFC2406600\/2022YFC2406604"],"award-info":[{"award-number":["2022YFC2406600\/2022YFC2406604"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the concordance of four anti-dsDNA antibody detection methods\u2014Crithidia luciliae indirect immunofluorescence test (CLIFT), enzyme-linked immunosorbent assay (ELISA), acridine ester direct chemiluminescence immunoassay (CLIA), and digital liquid chip method (DLCM)\u2014and to assess their diagnostic efficacy in systemic lupus erythematosus (SLE) patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 285 serum samples were collected, including 170 SLE patients, 39 with non-SLE autoimmune diseases (AIDs), 28 with non-AIDs, and 48 undiagnosed cases. The concordance and diagnostic performance of anti-dsDNA antibody methods were analyzed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The diagnostic performance showed that DLCM exhibited the highest sensitivity (86.87%), while CLIA demonstrated the highest specificity (94.03%). The area under the receiver operating characteristic (ROC) curve (AUC) was ranked as CLIFT &lt; ELISA &lt; CLIA &lt; DLCM (AUC = 0.938). Anti-dsDNA antibodies detected by both CLIFT and DLCM correlated well with the SLE disease activity index (SLEDAI), while CLIFT and CLIA were significantly correlated with lupus nephritis. Utilizing ROC curve-derived cut-off values, the overall concordance of CLIFT and other methods ranged from 80.14% to 82.58% (kappa &gt; 0.6,\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.001), and the concordance between quantitative methods ranged from 89.55% to 91.29% (kappa &gt; 0.8,\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CLIFT, ELISA, CLIA, and DLCM all showed impressive diagnostic efficacy in detecting anti-dsDNA antibodies. CLIFT shows a strong correlation with SLE activity and lupus nephritis. DLCM, a relatively new method, also showed excellent performance and could be integrated into clinical laboratory workflows for anti-dsDNA antibody testing.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09612033251371116","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T15:14:57Z","timestamp":1755962097000},"page":"1251-1260","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluating the performance of four anti-dsDNA antibody detection methods"],"prefix":"10.1177","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0775-0662","authenticated-orcid":false,"given":"Xuedan","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6812-407X","authenticated-orcid":false,"given":"Liqiong","family":"Wu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, Baoshan People\u2019s Hospital of Yunnan Province, Baoshan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0418-3521","authenticated-orcid":false,"given":"Yi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7042-561X","authenticated-orcid":false,"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2957-779X","authenticated-orcid":false,"given":"Zhuochun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5196-2707","authenticated-orcid":false,"given":"Junlong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5425-5285","authenticated-orcid":false,"given":"Zhenzhen","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0275-3722","authenticated-orcid":false,"given":"Bin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,8,23]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard-2022-223035"},{"key":"e_1_3_7_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2018-214819"},{"key":"e_1_3_7_4_2","first-page":"775","article-title":"Recommendations for diagnosis and treatment of systemic lupus erythematosus","volume":"62","author":"Shen N","year":"2023","unstructured":"Shen N, Zhao Y, Duan LH, et al. 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