{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:13:44Z","timestamp":1777929224085,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"13","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Yunnan Province Young and Middle\u2010aged Academic and Technical Leaders Reserve Talent Program","award":["202405AC350088"],"award-info":[{"award-number":["202405AC350088"]}]},{"name":"Doctoral Fund of the First People\u2019s Hospital of Yunnan Province","award":["KHBS-2022-027"],"award-info":[{"award-number":["KHBS-2022-027"]}]},{"DOI":"10.13039\/100007471","name":"Applied Basic Research Foundation of Yunnan Province","doi-asserted-by":"publisher","award":["Grant Number 202301AY070001-069"],"award-info":[{"award-number":["Grant Number 202301AY070001-069"]}],"id":[{"id":"10.13039\/100007471","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82060305"],"award-info":[{"award-number":["82060305"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:p>\n                    <jats:bold>Background<\/jats:bold>\n                    : Systemic lupus erythematosus (SLE) is an autoimmune disease often associated with anti-double stranded DNA (anti-dsDNA) antibodies and endothelial dysfunction, yet the mechanisms linking extracellular DNA to vascular injury remain unclear.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods<\/jats:bold>\n                    : We collected clinical samples from SLE patients and healthy controls, cultured human umbilical vein endothelial cells (HUVECs), and applied cell viability assays, ELISA, RT-qPCR, and Western blot to assess endothelial injury and activation pathways. We measured levels of von Willebrand factor (vWF), soluble thrombomodulin (sTM), and E-selectin in patient sera and cell culture supernatants.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    : SLE patients, particularly those positive for anti-dsDNA antibodies, showed significantly higher serum vWF, sTM, and E-selectin compared to controls (P &lt; 0.05). In vitro, exposure of HUVECs to dsDNA induced a dose- and time-dependent reduction in cell viability, with an IC50 of 2.874 \u03bcg\/ml, and significantly upregulated vWF, sTM, and E-selectin secretion. Mechanistically, dsDNA treatment activated the cGAS-STING-IRF3 signaling pathway, evidenced by increased mRNA and protein expression of cGAS, STING, and IRF3 (P &lt; 0.05), as well as enhanced cytoplasmic cGAS fluorescence intensity in immunofluorescence analysis. These results demonstrate that elevated extracellular dsDNA can directly damage endothelial cells through cGAS-STING-IRF3 pathway activation, mirroring the endothelial injury observed in SLE patients.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion<\/jats:bold>\n                    : Our findings suggest that cell-free dsDNA is a potent inducer of endothelial dysfunction, and that targeting the cGAS-STING-IRF3 axis may offer new therapeutic opportunities for SLE and other diseases marked by elevated extracellular DNA.\n                  <\/jats:p>","DOI":"10.1177\/09612033251386097","type":"journal-article","created":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T03:53:15Z","timestamp":1761969195000},"page":"1377-1385","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring endothelial dysfunction in SLE: cGAS-STING-IRF3 pathway activation by dsDNA"],"prefix":"10.1177","volume":"34","author":[{"given":"Zhou","family":"Yan","sequence":"first","affiliation":[{"name":"The First People\u2019s Hospital of Yunnan Province \/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Sanya Center for Disease Control and Prevention, Sanya, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zha","family":"Shun","sequence":"additional","affiliation":[{"name":"Yunnan Center for Disease Control and Prevention, Kunming, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhou","family":"Xiaoyu","sequence":"additional","affiliation":[{"name":"The First People\u2019s Hospital of Yunnan Province \/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mao","family":"Wenfei","sequence":"additional","affiliation":[{"name":"The First People\u2019s Hospital of Yunnan Province \/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Hong","sequence":"additional","affiliation":[{"name":"The First People\u2019s Hospital of Yunnan Province \/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0962-404X","authenticated-orcid":false,"given":"Zhang","family":"Ruixian","sequence":"additional","affiliation":[{"name":"The First People\u2019s Hospital of Yunnan Province \/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/0961203317734924"},{"key":"e_1_3_4_3_2","first-page":"13","article-title":"Anti-nucleosome antibody: significance in lupus patients lacking anti-double-stranded DNA antibody","volume":"20","author":"Min DJ","year":"2002","unstructured":"Min DJ, Kim SJ, Park SH, et al. 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