{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:42:32Z","timestamp":1777509752064,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"5-6","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:p>\n                    Hepatocyte transplantation is effective for treating liver failure, but healthy donors as a source of hepatocytes are quite limited. The livers of patients with hepatic fibrosis could be an alternative source; however, few reports have examined the nature of hepatocytes from fibrotic livers (f-hepatocytes). In this study, we compared the growth of f-hepatocytes and hepatocytes from normal livers (n-hepatocytes). Hepatocytes were isolated from normal and CCl\n                    <jats:sub>4<\/jats:sub>\n                    -treated wild-type Fischer rats that express dipeptidyl dipeptidase IV (DPPIV) gene (DPPIV\n                    <jats:sup>+<\/jats:sup>\n                    ). The n- and f-hepatocytes proliferated in culture at similar rates. Both types of hepatocytes were transplanted into DPPIV\n                    <jats:sup>-<\/jats:sup>\n                    mutant Fischer rats that had been treated with retrorsine to injure the liver and were partially hepatectomized (PHx) before transplantation. Both n- and f-DPPIV\n                    <jats:sup>+<\/jats:sup>\n                    -hepatocytes proliferated and formed colonies. The colony sizes of f-hepatocytes 21 days posttransplantation were approximately three times those of n-hepatocytes. The hepatocytes were analyzed using a fluorescence activated cell sorter (FACS). The FACS profile differed between f- and n-hepatocytes: f-hepatocytes were less granular, less autofluorescent, and smaller than n-hepatocytes. These characteristics of f-hepatocytes resembled those reported for small-sized n-hepatocytes (SHs), which are highly proliferative and preferentially express a unique set of 10 SH genes. However, f-hepatocytes preferentially expressed only five of the SH genes. The expression profile of f-hepatocytes was rather similar to that of proliferating n-hepatocytes in the regenerating liver after PHx. The f-hepatocytes were morphologically normal and did not show any preneoplastic phenotype. These normal and proliferative natures of f-hepatocytes in vivo suggest the fibrotic liver as a source of hepatocytes for transplantation.\n                  <\/jats:p>","DOI":"10.1177\/096368970901805-623","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T14:53:44Z","timestamp":1498143224000},"page":"665-676","source":"Crossref","is-referenced-by-count":4,"title":["Hepatocytes from Fibrotic Liver Possess High Growth Potential in Vivo"],"prefix":"10.1177","volume":"18","author":[{"given":"Manabu","family":"Nishie","sequence":"first","affiliation":[{"name":"Yoshizato Project, CLUSTER, Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima 739-0046, Japan"},{"name":"Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chise","family":"Tateno","sequence":"additional","affiliation":[{"name":"Yoshizato Project, CLUSTER, Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima 739-0046, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rie","family":"Utoh","sequence":"additional","affiliation":[{"name":"Yoshizato Project, CLUSTER, Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima 739-0046, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiko","family":"Kohashi","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Frontier Medical Science, Program for Biomedical Research, and Hiroshima University 21st Century COE Program for Advanced Radiation Casualty Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Masumoto","sequence":"additional","affiliation":[{"name":"Yoshizato Project, CLUSTER, Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima 739-0046, Japan"},{"name":"Department of Surgery, Division of Frontier Medical Science, Program for Biomedical Research, and Hiroshima University 21st Century COE Program for Advanced Radiation Casualty Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoya","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyuki","family":"Itamoto","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Frontier Medical Science, Program for Biomedical Research, and Hiroshima University 21st Century COE Program for Advanced Radiation Casualty Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriaki","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshimasa","family":"Asahara","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Frontier Medical Science, Program for Biomedical Research, and Hiroshima University 21st Century COE Program for Advanced Radiation Casualty Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsutoshi","family":"Yoshizato","sequence":"additional","affiliation":[{"name":"Yoshizato Project, CLUSTER, Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima 739-0046, Japan"},{"name":"Developmental Biology Laboratory and Hiroshima University 21st Century COE Program for Advanced Radiation Casualty Medicine, Department of Biological Science, Graduate School of Science, Hiroshima University, Hiroshima 739-8526, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2009,5,1]]},"reference":[{"key":"bibr1-096368970901805-623","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)61167-4"},{"key":"bibr2-096368970901805-623","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.02.076"},{"key":"bibr3-096368970901805-623","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-204-43660"},{"key":"bibr4-096368970901805-623","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199805143382004"},{"key":"bibr5-096368970901805-623","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/18.6.1169"},{"key":"bibr6-096368970901805-623","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63948-X"},{"key":"bibr7-096368970901805-623","doi-asserted-by":"publisher","DOI":"10.1080\/08977190290023913"},{"key":"bibr8-096368970901805-623","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65574-5"},{"key":"bibr9-096368970901805-623","doi-asserted-by":"publisher","DOI":"10.3727\/000000007783465019"},{"key":"bibr10-096368970901805-623","first-page":"585","volume":"11","author":"Mito 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