{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T03:50:50Z","timestamp":1793245850791,"version":"4.5.0"},"reference-count":39,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2018,12,17]],"date-time":"2018-12-17T00:00:00Z","timestamp":1545004800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:p>\n                    Bone non-union after fracture, considered a therapeutic challenge for orthopedics, always needs a reversion surgery, including autograft transplantation (AGT). However, adverse events related to autograft harvest cannot be ignored. Our group designed a novel system called the bone marrow stem cell Screen-Enrich-Combine Circulating System (SECCS) by seeding mesenchymal stem cells (MSCs) into \u03b2-tricalcium phosphate (\u03b2-TCP) during surgery to thereafter rapidly process bioactive bone implantation. In this retrospective case-control study, 30 non-union patients who accepted SECCS therapy and 20 non-union patients who accepted AGT were enrolled. By SECCS therapy, the MSC-enriched \u03b2-TCP particles were implanted into the non-union gap. During the enrichment procedure, a significant proportion of MSCs were screened and enriched from bone marrow into porous \u03b2-TCP particles, and the cells possessed the capacity for three-line differentiation and were CD90\n                    <jats:sup>+<\/jats:sup>\n                    \/CD105\n                    <jats:sup>+<\/jats:sup>\n                    \/CD34\n                    <jats:sup>-<\/jats:sup>\n                    \/CD45\n                    <jats:sup>-<\/jats:sup>\n                    . Approximately 82.0\u00b110.7% of MSCs were enriched from 60 mL bone marrow without damaging cell viability, and approximately 11,444.0\u00b16,018 MSCs were transplanted per patient. No implant-related infections occurred in any case. After 9 months of follow-up, 27 patients (90%) in the SECCS group acquired clinical union, compared with 18 patients (90%) in the AGT group (clinical union time, P = 0.064), and postoperative radiographic union score at 9 months post-operation was similar between the two groups. In conclusion, the SECCS could concentrate a large proportion of MSCs from bone marrow to acquire enough effective cells for therapy without\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    cell culture. Bone substitutes processed by SECCS demonstrated encouraging promotion of bone regeneration and showed a satisfactory clinical curative effect for diaphyseal bone non-union, which was non-inferior to AGT.\n                  <\/jats:p>","DOI":"10.1177\/0963689718818096","type":"journal-article","created":{"date-parts":[[2018,12,17]],"date-time":"2018-12-17T04:14:30Z","timestamp":1545020070000},"page":"212-223","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":29,"title":["Bone Mesenchymal Stem Cell-Enriched \u03b2-Tricalcium Phosphate Scaffold Processed by the Screen-Enrich-Combine Circulating System Promotes Regeneration of Diaphyseal Bone Non-Union"],"prefix":"10.1177","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5452-8597","authenticated-orcid":false,"given":"Xin","family":"Wang","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, Shang Hai, P.R. China"},{"name":"Department of Orthopaedic Surgery, Shanghai Ninth People\u2019s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, P.R. China."},{"name":"Both these authors contributed equally to this work as co-first authors."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WenXiang","family":"Chu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, Shang Hai, P.R. China"},{"name":"Department of Orthopaedic Surgery, Shanghai Ninth People\u2019s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, P.R. China."},{"name":"Both these authors contributed equally to this work as co-first authors."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YiFu","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, Shang Hai, P.R. China"},{"name":"Department of Orthopaedic Surgery, Shanghai Ninth People\u2019s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DingWei","family":"Shi","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, Shang Hai, P.R. China"},{"name":"Department of Orthopaedic Surgery, Shanghai Ninth People\u2019s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HaiRong","family":"Tao","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, Shang Hai, P.R. China"},{"name":"Department of Orthopaedic Surgery, Shanghai Ninth People\u2019s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Jin","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, Shang Hai, P.R. China"},{"name":"Department of Orthopaedic Surgery, Shanghai Ninth People\u2019s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KeRong","family":"Dai","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, Shang Hai, P.R. China"},{"name":"Department of Orthopaedic Surgery, Shanghai Ninth People\u2019s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, Shang Hai, P.R. China"},{"name":"Department of Orthopaedic Surgery, Shanghai Ninth People\u2019s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, P.R. 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