{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:05:31Z","timestamp":1777511131940,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Innovative Talents in Science and Technology of fund program of universities of Henan","doi-asserted-by":"publisher","award":["15HASTIT040"],"award-info":[{"award-number":["15HASTIT040"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:p>\n                    Human CD133\n                    <jats:sup>+<\/jats:sup>\n                    stem cells were injected into the bone marrow cavity of NOG (NOD Shi-SCID IL2R\u03b3c\n                    <jats:sup>null<\/jats:sup>\n                    ) mice with or without preconditioning of busulfan in order to assess the efficiency of human CD133\n                    <jats:sup>+<\/jats:sup>\n                    cells engraftment. Peripheral blood from CD133\n                    <jats:sup>+<\/jats:sup>\n                    -engrafted NOG mice was analyzed by flow cytometry. The results showed that human CD19\n                    <jats:sup>+<\/jats:sup>\n                    B lymphocytes could be detected at 4 weeks post-transplantation, and human CD4\n                    <jats:sup>+<\/jats:sup>\n                    , CD8\n                    <jats:sup>+<\/jats:sup>\n                    subsets of T lymphocytes, CD19\n                    <jats:sup>\u2013<\/jats:sup>\n                    CD14\n                    <jats:sup>\u2013<\/jats:sup>\n                    HLA-DR\n                    <jats:sup>+<\/jats:sup>\n                    DCs and CD19\n                    <jats:sup>\u2013<\/jats:sup>\n                    CD14\n                    <jats:sup>+<\/jats:sup>\n                    monocytes could be detected at 16 weeks post-transplantation. The survival rate of mice in busulfan-untreated group (100%) was slightly higher than that in the busulfan-pretreated group (83%) ( P &gt; 0.05). However, the differentiation efficiency of CD133\n                    <jats:sup>+<\/jats:sup>\n                    stem cells in busulfan-pretreated group was significantly higher than that in the untreated group ( P &lt; 0.05). This data imply that CD133\n                    <jats:sup>+<\/jats:sup>\n                    cells could be a good resource for a humanized mouse model, and the preconditioning of busulfan could be more conducive to accelerating the differentiation of human CD133\n                    <jats:sup>+<\/jats:sup>\n                    cells in NOG mice by intra-bone marrow injection.\n                  <\/jats:p>","DOI":"10.1177\/0963689719842162","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T07:37:05Z","timestamp":1555313825000},"page":"973-979","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["The Preconditioning of Busulfan Promotes Efficiency of Human CD133+ Cells Engraftment in NOD Shi-SCID IL2R\u03b3cnull (NOG) Mice via Intra-Bone Marrow Injection"],"prefix":"10.1177","volume":"28","author":[{"given":"Xiaofang","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Basic Medical Sciences, Xinxiang Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxiao","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, School of Laboratory Medicine, Xinxiang Medical University, China"},{"name":"Xinxiang Assegai Medical Laboratory Institute, School of Laboratory Medicine, Xinxiang Medical University, China"},{"name":"Henan Key Laboratory of Immunology and Targeted Drugs, Xinxiang Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, School of Laboratory Medicine, Xinxiang Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Third Affiliated Hospital of Xinxiang Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Henan Key Laboratory of Immunology and Targeted Drugs, Xinxiang Medical University, China"},{"name":"Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinming","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, School of Laboratory Medicine, Xinxiang Medical University, China"},{"name":"The Laboratory of Genetic Regulators in the immune system, School of Laboratory Medicine, Xinxiang Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, School of Laboratory Medicine, Xinxiang Medical University, China"},{"name":"Henan Key Laboratory of Immunology and Targeted Drugs, Xinxiang Medical University, China"},{"name":"Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,4,15]]},"reference":[{"key":"bibr1-0963689719842162","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2012.2"},{"key":"bibr2-0963689719842162","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2009.12.008"},{"issue":"3","key":"bibr3-0963689719842162","first-page":"464","volume":"120","author":"Custer RP","year":"1985","journal-title":"Am J 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