{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:56:53Z","timestamp":1777510613994,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31671252"],"award-info":[{"award-number":["31671252"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>Death domain\u2013associated protein (DAXX) is a multifunctional nuclear protein involved in apoptosis, transcription, deoxyribonucleic acid damage response, and tumorigenesis. However, the role of DAXX in breast cancer development and progression remains elusive. In this study, we examined the expression patterns and function of DAXX in human breast cancer samples and cell lines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Immunohistochemistry was used to analyze the expression and localization patterns of DAXX. Additionally, we investigated whether DAXX played an intrinsic role in the cellular response to damage induced by ultraviolet (UV) irradiation in MDA-MB-231 breast cancer cells (isolated at M D Anderson from a pleural effusion of a patient with invasive ductal carcinoma).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Our results showed that nucleus size, chromatin organization, and DAXX localization were altered in breast cancer tissues compared with those in control tissues. Compared with cytoplasmic and nuclear expression in benign breast tissues, DAXX was colocalized with promyelocytic leukemia in nuclei with a granular distribution. Endogenous DAXX messenger ribonucleic acid levels were upregulated upon UV radiation in MDA-MB-231 cells. DAXX-deficient cells tended to be more sensitive to irradiation than control cells. Conversely, DAXX-overexpressing cells exhibited reduced\u00a0phosphorylated histone H2AX (\u03b3-H2AX) accumulation, increased cell survival, and resistance to UV-induced damage. The protective effects of DAXX may be related to the activation of the ataxia telangiectasia mutated (ATM)-checkpoint kinase 2 (ATM-CHK2)-cell division cycle 25c (CDC25c) signaling pathways in Gap2\/Mitosis (G\n                      <jats:sub>2<\/jats:sub>\n                      \/M) checkpoint and ultimately cell cycle arrest at G\n                      <jats:sub>2<\/jats:sub>\n                      \/M phase.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Taken together, these results suggested that DAXX may be an essential component in breast cancer initiation, malignant progression, and radioresistance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0963689720920277","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T11:20:35Z","timestamp":1594725635000},"page":"096368972092027","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Enhanced UV Resistance Role of Death Domain\u2013Associated Protein in Human MDA-MB-231 Breast Cancer Cells by Regulation of G2 DNA Damage Checkpoint"],"prefix":"10.1177","volume":"29","author":[{"given":"Zhiyan","family":"Shan","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Harbin Medical University, Harbin, China"},{"name":"Both the authors contributed equally to this article"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Life Science, Wenzhou University, Wenzhou, Zhejiang, China"},{"name":"Both the authors contributed equally to this article"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingling","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Harbin Medical University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9921-0803","authenticated-orcid":false,"given":"Haiyue","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Oncology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honghong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Blood Transfusion Department of Sunshine Union Hospital, Weifang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Harbin Medical University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhipeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Oncology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 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