{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:58:40Z","timestamp":1777510720142,"version":"3.51.4"},"reference-count":31,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:p>Rapamycin (RAPA) and cisplatin (CDDP) are used as clinical drugs in the treatment of various tumors, but there are few studies on the combination of RAPA and CDDP. Tumor necrosis factor \u03b1 (TNF-\u03b1) plays an important role in tumorigenesis and development. This study is to explore the effects of RAPA combined with CDDP on the expression of TNF-\u03b1 in osteosarcoma MG-63 cells. MG-63 cells were routinely cultured and divided into a control group, a RAPA group (20 \u03bcM), a CDDP group (20 \u03bcM), and a RAPA + CDDP group (20 \u03bcM + 20 \u03bcM). The four groups were treated with drugs for 24 and 48 h, respectively. Real-time polymerase chain reaction (PCR), Western blot (WB), and immunocytochemistry (ICC) were adopted to detect the expression of TNF-\u03b1 gene and protein. The results of PCR showed that both the separate drug use and drug combination could significantly lower the relative expression quantity of TNF-\u03b1 gene (* P &lt; 0.5), but the combination was more effective (* P &lt; 0.5); the expression quantity of TNF-\u03b1 gene in the RAPA + CDDP group at 48 h was much lower than that at 24 h (*** P &lt; 0.001). The results of WB showed that both the separate drug use and drug combination could significantly lower the relative expression quantity of TNF-\u03b1 protein, and the combination was more effective than separate drug use (* P &lt; 0.05) and more effective at 48 h (*** P &lt; 0.001); the expression quantity of TNF-\u03b1 protein in the same group at 48 h was much lower than that at 24 h (* P &lt; 0.05). The results of ICC showed that both the separate drug use and drug combination could significantly lower the relative expression quantity of TNF-\u03b1 protein, and the combination was more effective than separate drug use (** P &lt; 0.01) and more effective at 48 h (*** P &lt; 0.001); the expression quantity of TNF-\u03b1 protein in the same group at 48 h was much lower than that at 24 h (** P &lt; 0.01). RAPA combined with CDDP can significantly reduce the expression of TNF-\u03b1 in MG-63 cells, which is time dependent.<\/jats:p>","DOI":"10.1177\/0963689720926153","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T08:32:28Z","timestamp":1595233948000},"page":"096368972092615","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Rapamycin Combined with Cisplatin on Tumor Necrosis Factor TNF-\u03b1 in MG-63 Cells"],"prefix":"10.1177","volume":"29","author":[{"given":"Tian-Yu","family":"Han","sequence":"first","affiliation":[{"name":"Department of Orthopaedics, General Hospital of Northern Theater Command, Shenyang, Liaoning, China"},{"name":"Both the authors contributed equally to this article"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Ma","sequence":"additional","affiliation":[{"name":"Division of Spine Surgery, Department of Orthopaedics, Tongji Hospital, Tongji University School of Medicine, Shanghai, China"},{"name":"Both the authors contributed equally to this article"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Shanghai Minhang TCM Hospital (Shanghai Minhang Hospital of Traditional Chinese Medicine), China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang-Bi","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, General Hospital of Northern Theater Command, Shenyang, Liaoning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9184-1707","authenticated-orcid":false,"given":"Xin-Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, General Hospital of Northern Theater Command, Shenyang, Liaoning, 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