{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T16:24:14Z","timestamp":1782750254270,"version":"3.54.5"},"reference-count":48,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"DuoGenic StemCells Corporation, Taiwan"},{"name":"Industry-Academic cooperation project","award":["108-D-595"],"award-info":[{"award-number":["108-D-595"]}]},{"name":"Industry-Academic cooperation project","award":["109-D-525"],"award-info":[{"award-number":["109-D-525"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>Cell expansion of human pluripotent stem cells (hPSCs) commonly depends on Matrigel as a coating matrix on two-dimensional (2D) culture plates and 3D microcarriers. However, the xenogenic Matrigel requires sophisticated quality-assurance processes to meet clinical requirements. In this study, we develop an innovative coating-free medium for expanding hPSCs. The xenofree medium supports the weekend-free culture and competitive growth of hPSCs on several cell culture plastics without an additional pre-coating process. The pluripotent stemness of the expanded cells is stably sustained for more than 10 passages, featured with high pluripotent marker expressions, normal karyotyping, and differentiating capacity for three germ layers. The expression levels of some integrins are reduced, compared with those of the hPSCs on Matrigel. This medium also successfully supports the clonal expansion and induced pluripotent stem cell establishment from mitochondrial-defective MELAS (mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes) patient\u2019s peripheral blood mononuclear cells. This innovative hPSC medium provides a straightforward scale-up process for producing clinical-orientated hPSCs by excluding the conventional coating procedure.<\/jats:p>","DOI":"10.1177\/09636897231198172","type":"journal-article","created":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T07:35:29Z","timestamp":1694504129000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells"],"prefix":"10.1177","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4557-242X","authenticated-orcid":false,"given":"Chih-Yao","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Yun","family":"Ching","sequence":"additional","affiliation":[{"name":"Duogenic StemCells Corporation, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih-Fang","family":"Wu","sequence":"additional","affiliation":[{"name":"The Joint Program of Tissue Engineering and Regenerative Medicine, National Chung Hsing University and National Health Research Institutes, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ko","family":"Lee","sequence":"additional","affiliation":[{"name":"Duogenic StemCells Corporation, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3485-7558","authenticated-orcid":false,"given":"Hueng-Chuen","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Tungs\u2019 Taichung MetroHarbor Hospital, Wuchi, Taichung, Taiwan"},{"name":"Department of Nursing, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5804-6966","authenticated-orcid":false,"given":"Liang-Yu","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Life Science, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su-Yi","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Life Science, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ching","family":"Chen","sequence":"additional","affiliation":[{"name":"The Joint Program of Tissue Engineering and Regenerative Medicine, National Chung Hsing University and National Health Research Institutes, Taichung, Taiwan"},{"name":"Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua City, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-I","family":"Shen","sequence":"additional","affiliation":[{"name":"Duogenic StemCells Corporation, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7734-3499","authenticated-orcid":false,"given":"Hong-Lin","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,9,12]]},"reference":[{"key":"bibr1-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1177\/09636897211017829"},{"key":"bibr2-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1177\/09636897221104451"},{"key":"bibr3-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1177\/09636897211014820"},{"key":"bibr4-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1177\/0963689720988245"},{"key":"bibr5-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.101530"},{"key":"bibr6-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.07.037"},{"key":"bibr7-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120017"},{"key":"bibr8-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.119638"},{"key":"bibr9-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1038\/srep41165"},{"key":"bibr10-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12170"},{"key":"bibr11-09636897231198172","first-page":"41","author":"Esfahani SN","year":"2021","journal-title":"Nano Today"},{"key":"bibr12-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.35034"},{"key":"bibr13-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.2c00162"},{"issue":"6","key":"bibr14-09636897231198172","volume":"10","author":"Nasir A","year":"2021","journal-title":"Adv Healthc Mater"},{"key":"bibr15-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1038\/srep18136"},{"key":"bibr16-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.02.012"},{"key":"bibr17-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.01.015"},{"key":"bibr18-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1021\/nn3039148"},{"key":"bibr19-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.10.031"},{"issue":"5","key":"bibr20-09636897231198172","volume":"10","author":"Zand\u00e9n C","year":"2014","journal-title":"Nanomedicine"},{"issue":"2","key":"bibr21-09636897231198172","doi-asserted-by":"crossref","DOI":"10.1042\/bj3350343","volume":"335","author":"Estape D","year":"1998","journal-title":"Biochem J"},{"key":"bibr22-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1021\/bi00070a004"},{"key":"bibr23-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.04.043"},{"key":"bibr24-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060298"},{"key":"bibr25-09636897231198172","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232113462"},{"key":"bibr26-09636897231198172","doi-asserted-by":"publisher","DOI":"10.3390\/cells10102773"},{"key":"bibr27-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1038\/srep08744"},{"key":"bibr28-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvz148"},{"key":"bibr29-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1002\/cpsc.20"},{"key":"bibr30-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.081679"},{"key":"bibr31-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2428"},{"key":"bibr32-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-014-9509-0"},{"key":"bibr33-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0291"},{"key":"bibr34-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0328"},{"key":"bibr35-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1021"},{"key":"bibr36-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2018.07.020"},{"key":"bibr37-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5380560"},{"key":"bibr38-09636897231198172","doi-asserted-by":"publisher","DOI":"10.3390\/cells8010065"},{"key":"bibr39-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1038\/nature14546"},{"key":"bibr40-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1311660110"},{"key":"bibr41-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1389"},{"key":"bibr42-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1039\/D1TB01878G"},{"key":"bibr43-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1186\/2045-9769-2-7"},{"key":"bibr44-09636897231198172","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00070"},{"key":"bibr45-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136350"},{"key":"bibr46-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1116\/1.3525804"},{"key":"bibr47-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.580076"},{"key":"bibr48-09636897231198172","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddv201"}],"container-title":["Cell Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09636897231198172","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09636897231198172","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09636897231198172","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:39:42Z","timestamp":1777390782000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09636897231198172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":48,"alternative-id":["10.1177\/09636897231198172"],"URL":"https:\/\/doi.org\/10.1177\/09636897231198172","relation":{},"ISSN":["0963-6897","1555-3892"],"issn-type":[{"value":"0963-6897","type":"print"},{"value":"1555-3892","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1]]},"article-number":"09636897231198172"}}