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We explore the role of NogoA\/NgR\/Rho pathway in mediating hUCMSC to improve neurobehavioral status and alleviate brain injury in hypoxia\/ischemia-induced CP (cerebral palsy) rat model in order to promote the clinical application of stem cell therapy in CP. The injury model of HT22 cells was established after 3 h hypoxia, and then co-cultured with hUCMSC. The rat model of CP was established by ligation of the left common carotid artery for 2.5 h. Subsequently, hUCMSC was administered via the tail vein once a week for a total of four times. The neurobehavioral status of CP rats was determined by behavioral experiment, and the pathological brain injury was determined by pathological staining method. The mRNA and protein expressions of NogoA, NgR, RhoA, Rac1, and CDC42 in brain tissues of rats in all groups and cell groups were detected by real-time quantitative polymerase chain reaction (RT-qPCR), Western blot, and immunofluorescence. The CP rats exhibited obvious motor function abnormalities and pathological damage. Compared with the control group, hUCMSC transplantation could significantly improve the neurobehavioral situation and attenuate brain pathological injury in CP rats. The relative expression of NogoA, NgR, RhoA mRNA, and protein in brain tissues of rats in the CP group was significantly higher than the rats in the sham and CP+hUCMSC group. The relative expression of Rac1, CDC42 mRNA, and protein in brain tissues of rats in the CP group was significantly lower than the rats in the sham and CP+hUCMSC group. The animal experiment results were consistent with the experimental trend of hypoxic injury of HT22 cells. This study confirmed that hUCMSC can efficiently improve neurobehavioral status and alleviate brain injury in hypoxia\/ischemia-induced CP rat model and HT22 cell model through downregulating the NogoA\/NgR\/Rho pathway.<\/jats:p>","DOI":"10.1177\/09636897231210069","type":"journal-article","created":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T05:52:27Z","timestamp":1700459547000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Human Umbilical Cord Mesenchymal Stem Cells Improve the Status of Hypoxic\/Ischemic Cerebral Palsy Rats by Downregulating NogoA\/NgR\/Rho Pathway"],"prefix":"10.1177","volume":"32","author":[{"given":"Yaoling","family":"Luo","sequence":"first","affiliation":[{"name":"Subcenter for Stem Cell Clinical Translation, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"},{"name":"Clinical Medicine Research Center, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"},{"name":"Ganzhou Key Laboratory of Stem Cell and Regenerative Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2245-7250","authenticated-orcid":false,"given":"Jiayang","family":"Qu","sequence":"additional","affiliation":[{"name":"Subcenter for Stem Cell Clinical Translation, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"},{"name":"School of Rehabilitation Medicine Gannan Medical University, Ganzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengyi","family":"He","sequence":"additional","affiliation":[{"name":"Subcenter for Stem Cell Clinical Translation, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"},{"name":"Clinical Medicine Research Center, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"},{"name":"Ganzhou Key Laboratory of Stem Cell and Regenerative Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Subcenter for Stem Cell Clinical Translation, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"},{"name":"Clinical Medicine Research Center, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"},{"name":"Ganzhou Key Laboratory of Stem Cell and Regenerative Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengwei","family":"Zou","sequence":"additional","affiliation":[{"name":"Subcenter for Stem Cell Clinical Translation, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"},{"name":"Clinical Medicine Research Center, First Affiliated Hospital of Gannan Medical University, Ganzhou, 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Cell Clinical Translation, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"},{"name":"Ganzhou Key Laboratory of Stem Cell and Regenerative Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, China"},{"name":"Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, China"},{"name":"Key Laboratory of Biomaterials and Biofabrication in Tissue Engineering of Jiangxi Province, Gannan Medical University, Ganzhou, 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