{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T17:14:45Z","timestamp":1793639685918,"version":"4.5.1"},"reference-count":29,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG8K1541"],"award-info":[{"award-number":["CMRPG8K1541"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      We tested the hypothesis that overexpression of cellular-prion-protein in adipose-derived mesenchymal stem cells (PrPC\n                      <jats:sup>OE<\/jats:sup>\n                      -ADMSCs) effectively protected the kidney against ischemia-reperfusion (IR) injury in rat.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Part I of cell culture was categorized into A1(ADMSCs)\/A2(ADMSCs+p-Cresol)\/A3(PrPC\n                      <jats:sup>OE<\/jats:sup>\n                      in ADMSCs)\/A4 (PrPC\n                      <jats:sup>OE<\/jats:sup>\n                      in ADMSCs+p-Cresol). Part II of cell culture was divided into B1(ADMSCs)\/B2[ADMSCs+lipopolysaccharide (LPS)]\/B3(PrPC\n                      <jats:sup>OE<\/jats:sup>\n                      in ADMSCs)\/B4(PrPC\n                      <jats:sup>OE<\/jats:sup>\n                      in ADMSCs+LPS). Sprague-Dawley (SD) rats (n = 50) were equally categorized into groups 1 (sham-operated-control)\/2 (IR)\/3 (IR+ADMSCs\/6.0 \u00d7 10\n                      <jats:sup>5<\/jats:sup>\n                      equally divided into bilateral-renal arteries and 6.0 \u00d7 10\n                      <jats:sup>5<\/jats:sup>\n                      intravenous administration by 1 h after IR)\/4 [IR+PrPC\n                      <jats:sup>OE<\/jats:sup>\n                      -ADMSCs (identical dosage administered as group 3)]\/5 [IR+silencing PRNP -ADMSCs (identical dosage administered as group 3)], and kidneys were harvested post-day 3 IR injury.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Part I results demonstrated that the cell viability at 24\/48\/72 h, BrdU uptake\/number of mitDNA\/APT concentration\/mitochondrial-cytochrome-C+ cells and the protein expressions of ki67\/PrPC at 72 h-cell culturing were significantly higher in PrPC\n                      <jats:sup>OE<\/jats:sup>\n                      -ADMSCs than in ADMSCs (all P &lt; 0.001). The protein expressions of oxidative-stress (NOX-1\/NOX2\/NOX4\/oxidized protein)\/mitochondrial-damaged (p22-phox\/cytosolic-cytochrome-C)\/inflammatory (p-NF-\u03baB\/IL-1\u00df\/TNF-\u03b1\/IL-6)\/apoptotic (cleaved caspase-3\/cleaved-PARP) biomarkers were lowest in A1\/A3 and significantly higher in A2 than in A4 (all P &lt; 0.001). Part II result showed that the protein expressions of inflammatory (p-NF-\u03baB\/IL-1\u00df\/TNF-\u03b1\/IL-6)\/apoptotic (cleaved caspase-3\/cleaved-PARP) biomarkers exhibited an identical pattern of part I among the groups (all P &lt; 0.001). The protein expressions of inflammatory (p-NF-\u03baB\/IL-1\u00df\/TNF-\u03b1\/MMP-9)\/oxidative-stress (NOX-1\/NOX-2\/oxidized-protein)\/mitochondrial-damaged (cytosolic-cytochrome-C\/p22-phox)\/apoptotic (cleaved caspase-3\/cleaved-PARP\/mitochondrial-Bx)\/autophagic (beclin-1\/ratio of LC3B-II\/LC3B-I)\/fibrotic (Smad3\/TGF-\u00df) biomarkers and kidney-injury-score\/creatinine level were lowest in group 1, highest in group 2, significantly higher in group 5 than in groups 3\/4 (all P &lt; 0.0001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      PrPC\n                      <jats:sup>OE<\/jats:sup>\n                      in ADMSCs rejuvenated these cells and played a cardinal role on protecting the kidney against IR injury.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09636897231211067","type":"journal-article","created":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T07:58:28Z","timestamp":1702281508000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Prion Protein Overexpression in Adipose-Derived Mesenchymal Stem Cells (ADMSCs) Effectively Protected Rodent Kidney Against Ischemia-Reperfusion Injury Via Enhancing ATP\/Mitochondrial Biogenesis\u2014Role of ADMSC Rejuvenation and Proliferation"],"prefix":"10.1177","volume":"32","author":[{"given":"Yen-Ta","family":"Chen","sequence":"first","affiliation":[{"name":"Division of Urology, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung"},{"name":"Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Chao","family":"Yang","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Y.","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Computer Science and Engineering, National Sun Yat-Sen University, Kaohsiung"},{"name":"Department of Healthcare Administration and Medical Informatics, Kaohsiung Medical University, Kaohsiung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Hsun","family":"Sung","sequence":"additional","affiliation":[{"name":"Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung"},{"name":"Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung"},{"name":"Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Lin","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Nursing, Asia University, Taichung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Ruei","family":"Huang","sequence":"additional","affiliation":[{"name":"Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung"},{"name":"Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mel S.","family":"Lee","sequence":"additional","affiliation":[{"name":"Paochien Hospital, Pingtung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6305-5717","authenticated-orcid":false,"given":"Hon-Kan","family":"Yip","sequence":"additional","affiliation":[{"name":"Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung"},{"name":"Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung"},{"name":"Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung"},{"name":"Department of Nursing, Asia University, Taichung"},{"name":"Department of Medical Research, China Medical University Hospital, China Medical University, Taichung"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,12,11]]},"reference":[{"key":"bibr1-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2004090740"},{"key":"bibr2-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0b013e32834cd349"},{"key":"bibr3-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2008.11.034"},{"key":"bibr4-09636897231211067","volume":"99","author":"Friedericksen DV","year":"2009","journal-title":"S Afr Med J"},{"key":"bibr5-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2011.08.016"},{"key":"bibr6-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2014.09.008"},{"key":"bibr7-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1159\/000430770"},{"key":"bibr8-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1159\/000431337"},{"key":"bibr9-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1159\/000186651"},{"key":"bibr10-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1007\/s001340051281"},{"key":"bibr11-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3181a40a0b"},{"key":"bibr12-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.800011"},{"key":"bibr13-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009060636"},{"key":"bibr14-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2013.09.012"},{"key":"bibr15-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.928796"},{"key":"bibr16-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-9-51"},{"key":"bibr17-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12140"},{"key":"bibr18-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00020.2004"},{"key":"bibr19-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00270.2006"},{"key":"bibr20-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-9-11"},{"key":"bibr21-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45161"},{"key":"bibr22-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-11-270"},{"key":"bibr23-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1186\/scrt212"},{"key":"bibr24-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-9-118"},{"key":"bibr25-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12020"},{"issue":"5","key":"bibr26-09636897231211067","first-page":"781","volume":"7","author":"Chen YL","year":"2015","journal-title":"Am J Transl Res"},{"issue":"2","key":"bibr27-09636897231211067","volume":"7","author":"Sun CK","year":"2015","journal-title":"Am J Transl Res"},{"key":"bibr28-09636897231211067","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112551"},{"key":"bibr29-09636897231211067","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10010167"}],"container-title":["Cell Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09636897231211067","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09636897231211067","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09636897231211067","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:39:49Z","timestamp":1777390789000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09636897231211067"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":29,"alternative-id":["10.1177\/09636897231211067"],"URL":"https:\/\/doi.org\/10.1177\/09636897231211067","relation":{},"ISSN":["0963-6897","1555-3892"],"issn-type":[{"value":"0963-6897","type":"print"},{"value":"1555-3892","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1]]},"article-number":"09636897231211067"}}