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This usually leads to uterine infertility, menstrual disorders, and placental abnormalities. While stem cell therapy has shown extensive progress in repairing the damaged endometrium and preventing intrauterine adhesion, issues of low engraftment rates, rapid senescence, and the risk of tumorigenesis remain to be resolved for efficient and effective application of this technology in endometrial repair. This study addressed these challenges by developing a co-culture system to generate multi-lineage endometrial organoids (MLEOs) comprising endometrial epithelium organoids (EEOs) and endometrial mesenchymal stem cells (eMSCs). The efficacy of these MLEOs was investigated by seeding them on a biocompatible scaffold, the human acellular amniotic membrane (HAAM), to create a biological graft patch, which was subsequently transplanted into an injury model of the endometrium in rats. The results indicated that the MLEOs on the HAAM patch facilitated endometrial angiogenesis, regeneration, and improved pregnancy outcomes. The MLEOs on the HAAM patch could serve as a promising strategy for treating endometrial injury and preventing Asherman\u2019s syndrome.<\/jats:p>","DOI":"10.1177\/09636897231218408","type":"journal-article","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T20:51:16Z","timestamp":1702587076000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":26,"title":["Multi-Lineage Human Endometrial Organoids on Acellular Amniotic Membrane for Endometrium Regeneration"],"prefix":"10.1177","volume":"32","author":[{"given":"Yuhui","family":"Xu","sequence":"first","affiliation":[{"name":"Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China"},{"name":"Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyan","family":"Cai","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology 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