{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T01:37:08Z","timestamp":1781055428033,"version":"3.54.1"},"reference-count":24,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT) Japan","doi-asserted-by":"crossref","award":["Grants-in-Aids #21H02978"],"award-info":[{"award-number":["Grants-in-Aids #21H02978"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT) Japan","doi-asserted-by":"crossref","award":["Grants-in-Aids, #23H03304"],"award-info":[{"award-number":["Grants-in-Aids, #23H03304"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT) Japan","doi-asserted-by":"crossref","award":["Grants-in-Aids, #24K02501"],"award-info":[{"award-number":["Grants-in-Aids, #24K02501"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Type 1 diabetes mellitus (T1DM) affects 8.4 million people worldwide, with patients primarily relying on exogenous insulin injections to maintain blood glucose levels. Islet transplantation via the portal vein has allowed for the direct internal release of insulin by glucose-sensitive islets. However, this method might not be desirable for future cell therapy transplanting pluripotent stem cell-derived \u03b2 cells, facing challenges including difficulties in cell retrieval and graft loss due to the instant blood-mediated inflammatory reaction (IBMIR). Here, we established a subcutaneous transplantation protocol using an atelocollagen sponge as a scaffold. While the subcutaneous site has many advantages, the lack of a vascular bed limits its application. To address this issue, we performed angiogenesis stimulation at the transplantation site using bFGF absorbed in a gelatin sponge (Spongel), significantly improving the microvascular area. Our in vivo experiments also revealed angiogenesis stimulation is crucial for reversing hyperglycemia in streptozotocin (STZ)-induced diabetic mice. In addition to the angiogenic treatment, an atelocollagen sponge is used to carry the islets and helps avoid graft leakage. With 800 mouse islets delivered by the atelocollagen sponge, the STZ-induced diabetic mice showed a reversal of hyperglycemia and normalized glucose intolerance. Their normoglycemia was maintained until the graft was removed. Analysis of the harvested islet grafts exhibited a high vascularization and preserved morphologies, suggesting that using an atelocollagen sponge as a scaffold helps maintain the viability of the islet grafts.<\/jats:p>","DOI":"10.1177\/09636897241277980","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T04:33:15Z","timestamp":1727670795000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Reversal of Hyperglycemia by Subcutaneous Islet Engraftment Using an Atelocollagen Sponge as a Scaffold"],"prefix":"10.1177","volume":"33","author":[{"given":"Yumeng","family":"Wu","sequence":"first","affiliation":[{"name":"School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Yano","sequence":"additional","affiliation":[{"name":"Daiichi Sankyo Co., Ltd., Shinagawa-ku, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Enomoto","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan"},{"name":"Biomaterials Analysis Division, Open Facility Center, Tokyo Institute of Technology, Yokohama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atena","family":"Endo","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiji","family":"Okada","sequence":"additional","affiliation":[{"name":"Division of Hematopoiesis, Joint Research Center for Retroviral Infection, Kumamoto University, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimi","family":"Araki","sequence":"additional","affiliation":[{"name":"Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan"},{"name":"Center for Metabolic Regulation of Healthy Aging, Kumamoto University, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuaki","family":"Shiraki","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4292-205X","authenticated-orcid":false,"given":"Shoen","family":"Kume","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,9,29]]},"reference":[{"key":"bibr1-09636897241277980","doi-asserted-by":"crossref","unstructured":"Ogrotis I, Koufakis T, Kotsa K. Changes in the global epidemiology of type 1 diabetes in an evolving landscape of environmental factors: causes, challenges, and opportunities. Med. 2023;59(4):668.","DOI":"10.3390\/medicina59040668"},{"key":"bibr2-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1900\/RDS.2012.9.385"},{"key":"bibr3-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2018.02.138"},{"key":"bibr4-09636897241277980","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19051391"},{"key":"bibr5-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2023.100959"},{"key":"bibr6-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.9b03605"},{"key":"bibr7-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1126\/science.2119056"},{"key":"bibr8-09636897241277980","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12113735"},{"key":"bibr9-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117561"},{"issue":"6","key":"bibr10-09636897241277980","volume":"22","author":"Bertuzzi F","year":"2018","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"bibr11-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-020-0269-7"},{"key":"bibr12-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3211"},{"key":"bibr13-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000001970"},{"key":"bibr14-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1619216114"},{"key":"bibr15-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12373-5"},{"key":"bibr16-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1177\/0963689718797570"},{"key":"bibr17-09636897241277980","first-page":"539748","volume":"2011","author":"Ono A","year":"2011","journal-title":"J Biomed Biotechnol"},{"key":"bibr18-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1177\/09636897231186063"},{"key":"bibr19-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-39212-4"},{"key":"bibr20-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-68987-z"},{"key":"bibr21-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-013-9638-3"},{"key":"bibr22-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2003.00660.x"},{"key":"bibr23-09636897241277980","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.20016"},{"key":"bibr24-09636897241277980","doi-asserted-by":"publisher","DOI":"10.2485\/jhtb.18.89"}],"container-title":["Cell Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09636897241277980","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09636897241277980","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09636897241277980","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:40:07Z","timestamp":1777390807000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09636897241277980"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":24,"alternative-id":["10.1177\/09636897241277980"],"URL":"https:\/\/doi.org\/10.1177\/09636897241277980","relation":{},"ISSN":["0963-6897","1555-3892"],"issn-type":[{"value":"0963-6897","type":"print"},{"value":"1555-3892","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"article-number":"09636897241277980"}}