{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:46:43Z","timestamp":1777510003674,"version":"3.51.4"},"reference-count":44,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100011892","name":"Kaohsiung Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG8M0951"],"award-info":[{"award-number":["CMRPG8M0951"]}],"id":[{"id":"10.13039\/501100011892","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:p>\n                    This study revealed that adipose-derived mesenchymal stem cell-facilitated ciprofloxacin therapy effectively protected the kidney parenchyma and functional integrity against acute pyelonephritis damage in rodents. In vitro studies revealed that adipose-derived mesenchymal stem cell-derived media significantly suppressed the number of bacterial colony formation units (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.0001). Additionally, the combination of adipose-derived mesenchymal stem cell-ciprofloxacin was superior to either treatment alone on suppressing lipopolysaccharide-induced inflammatory reactions in macrophages and peripheral blood-derived mononuclear cells and attenuated lipopolysaccharide-induced apoptosis\/DNA damage in uroepithelial cells (Simian virus Hydrologic Unit Code 1) (all\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.0001). Sprague-Dawley rats were categorized into groups 1 (sham-control)\/2 (acute pyelonephritis)\/3 (acute pyelonephritis-ciprofloxacin)\/4 (acute pyelonephritis- adipose-derived mesenchymal stem cell)\/5 (acute pyelonephritis-adipose-derived mesenchymal stem cell-ciprofloxacin), and kidneys were harvested by day 5 after acute pyelonephritis induction. The in vivo results revealed that the day-5 mortality rate and creatinine levels at days 2 and 5 were significantly greater in group 2 than in groups 1 and 5 (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    = 0.01), whereas the kidney injury score and inflammatory cell infiltration in the kidney were highest in group 2, lowest in group 1, and significantly greater in groups 3 and 4 than in group 5; however, there was no difference between groups 3 and 4 (all\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.0001). The upstream inflammatory signaling (toll-like receptor-4, myeloid differentiation primary response 88, tumor necrosis factor receptor associated factor 6 and nuclear factor-kappa B) and downstream inflammatory signaling (tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6) biomarkers exhibited identical patterns of kidney injury scores among the groups (all\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.0001). The results of the present study showed that adipose-derived mesenchymal stem cell-facilitated ciprofloxacin reduced inflammatory signaling-induced kidney parenchymal damage and acute pyelonephritis-induced mortality and preserved kidney function.\n                  <\/jats:p>","DOI":"10.1177\/09636897251344851","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:44:03Z","timestamp":1750812243000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Adipose-derived mesenchymal stem cell-supported ciprofloxacin therapy effectively protects the kidney parenchyma and functional integrity against acute pyelonephritis damage in rodents"],"prefix":"10.1177","volume":"34","author":[{"given":"Chih-Chao","family":"Yang","sequence":"first","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya","family":"Yue","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-Lin","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Nursing, Asia University, Taichung"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ben-Chung","family":"Cheng","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuen-Wei","family":"Hsu","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Ling","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung"},{"name":"Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheung-Fat","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Radiology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6305-5717","authenticated-orcid":false,"given":"Hon-Kan","family":"Yip","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung"},{"name":"Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung"},{"name":"Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung"},{"name":"Department of Medical Research, China Medical University Hospital, China Medical University, Taichung"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mel S.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung"},{"name":"Paochien Hospital, Pingtung"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,6,24]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1702758"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/519268"},{"issue":"5","key":"e_1_3_3_4_2","article-title":"Diagnosis and treatment of acute pyelonephritis in women","volume":"84","author":"Colgan R","year":"2011","unstructured":"Colgan R, Williams M, Johnson JR. Diagnosis and treatment of acute pyelonephritis in women. Am Fam Physician. 2011;84(5):519\u201326.","journal-title":"Am Fam Physician"},{"key":"e_1_3_3_5_2","volume-title":"Pyelonephritis (acute): antimicrobial prescribing","author":"NICE","year":"2018","unstructured":"NICE. Pyelonephritis (acute): antimicrobial prescribing [NICE guideline (NG11)]. 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Practitioner. 2010;254(1728):19, 21, 23\u201324, 2.","journal-title":"Practitioner"},{"issue":"4","key":"e_1_3_3_14_2","article-title":"Severe acute pyelonephritis: a review of clinical outcome and risk factors for mortality","volume":"20","author":"Chung VY","year":"2014","unstructured":"Chung VY, Tai CK, Fan CW, Tang CN. Severe acute pyelonephritis: a review of clinical outcome and risk factors for mortality. 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