{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T12:15:33Z","timestamp":1792844133872,"version":"4.5.0"},"reference-count":19,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Bio and Medical Technology Development Program of the National Research Foundation of Korea","award":["NRF-2018M3A9B5023052"],"award-info":[{"award-number":["NRF-2018M3A9B5023052"]}]},{"name":"Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korea government","award":["22C0609L1"],"award-info":[{"award-number":["22C0609L1"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:p>Mitochondrial transplantation has emerged as a promising strategy for treating ischemic diseases by restoring mitochondrial function in damaged tissues. This study investigated the therapeutic potential of mitochondria isolated from placenta-derived mesenchymal stem cells (PD-MSCs) in a murine critical limb ischemia (CLI) model. The isolated mitochondria were characterized to confirm their structural integrity, purity, and ATP production capacity before transplantation into an ischemic hindlimb. Results showed that mitochondrial transplantation significantly improved blood flow and muscle regeneration compared with MSC transplantation, as evidenced by laser Doppler perfusion imaging and histological analysis. Enhanced ATP production and increased oxidative phosphorylation complex protein levels were observed, supporting energy metabolism in ischemic conditions. Mitochondrial transplantation also reduced mitochondrial reactive oxygen species (mROS) levels and increased antioxidant enzyme expression, including SOD-2, leading to reduced oxidative stress and apoptosis, as indicated by decreased Bax, cytosolic cytochrome c, and cleaved caspase-3 levels. Furthermore, mitochondrial transplantation promoted angiogenesis and increased vascular density in ischemic muscles by enhancing endothelial cell function. Overall, PD-MSC-derived mitochondrial transplantation demonstrated proved more effective over MSC transplantation in reducing inflammation, restoring mitochondrial function, and supporting tissue recovery, highlighting its promise as an effective therapeutic approach for CLI and other ischemic conditions by directly addressing mitochondrial dysfunction and overcoming the limitations of conventional cell therapies.<\/jats:p>","DOI":"10.1177\/09636897251347391","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T07:34:15Z","timestamp":1749713655000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparative analysis of mitochondrial and mesenchymal stem cell transplantation for angiogenesis and muscle regeneration"],"prefix":"10.1177","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0594-5703","authenticated-orcid":false,"given":"Mi Jin","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biotechnology, CHA University, Seongnam-si, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0707-1939","authenticated-orcid":false,"given":"Jung Wook","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, CHA University, Seongnam-si, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2179-2794","authenticated-orcid":false,"given":"Chang-Koo","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, CHA University, Seongnam-si, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9088-2577","authenticated-orcid":false,"given":"Ikhyun","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Bio Convergence Science, Graduate School, CHA University, Seongnam-si, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3357-9795","authenticated-orcid":false,"given":"Kyunghoon","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam-si, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8445-8067","authenticated-orcid":false,"given":"Yong-Soo","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, CHA University, Seongnam-si, Republic of Korea"},{"name":"Department of Bio Convergence Science, Graduate School, CHA University, Seongnam-si, Republic of Korea"},{"name":"Department of Life Science, Graduate School, CHA University, Seongnam-si, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2006.12.037"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI26987"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.2165\/00002018-199513020-00002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1053\/ejvs.2002.1615"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1051-0443(07)61332-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/4954070"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00356.2015"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2014.02.013"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-023-09643-7"},{"issue":"2","key":"e_1_3_2_11_2","article-title":"Mitochondrial ATP production is required for endothelial cell control of vascular tone","volume":"4","author":"Wilson C","year":"2023","unstructured":"Wilson C, Lee MD, Buckley C, Zhang X, McCarron JG. Mitochondrial ATP production is required for endothelial cell control of vascular tone. Function (Oxf). 2023;4(2):zqac063.","journal-title":"Function (Oxf)"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.834754"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9121304"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2013.07.100"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/med.21986"},{"issue":"6","key":"e_1_3_2_16_2","article-title":"Chronic stress-induced apoptosis is mitigated by young mitochondria transplantation in the prefrontal cortex of aged rats","volume":"26","author":"Javani G","year":"2023","unstructured":"Javani G, Ghaffari-Nasab A, Farajdokht F, Mohaddes G. Chronic stress-induced apoptosis is mitigated by young mitochondria transplantation in the prefrontal cortex of aged rats. Iran J Basic Med Sci. 2023;26(6):725\u201330.","journal-title":"Iran J Basic Med Sci"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00883.2012"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-023-02759-0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2017.03.004"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2017.02.018"}],"container-title":["Cell Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09636897251347391","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09636897251347391","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09636897251347391","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:36:11Z","timestamp":1777390571000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09636897251347391"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,12]]},"references-count":19,"alternative-id":["10.1177\/09636897251347391"],"URL":"https:\/\/doi.org\/10.1177\/09636897251347391","relation":{},"ISSN":["0963-6897","1555-3892"],"issn-type":[{"value":"0963-6897","type":"print"},{"value":"1555-3892","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,12]]},"article-number":"09636897251347391"}}