{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:46:52Z","timestamp":1777510012592,"version":"3.51.4"},"reference-count":45,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Double Innovation Talent Program of Jiangsu Province","award":["2017 (Y.H.)"],"award-info":[{"award-number":["2017 (Y.H.)"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571193 (Y.H.)"],"award-info":[{"award-number":["81571193 (Y.H.)"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:p>Schwann cell (SC) transplantation is considered as a promising strategy for spinal cord injury. However, SCs show less capability in assisting the regenerative axons to penetrate through astrocyte (AS)-formed scar barrier. Anosmin-1, an extracellular matrix glycosylated adhesion protein expressed in the olfactory bulb, is involved in olfactory ensheathing cells and reborn olfactory nerve axons continually penetrating the glial barrier and targeting the olfactory bulb. In this study, we employ a dorsal root crush injury model treated with anosmin-1. A vertical climbing test was used for behavioral analysis and immunohistochemical study for SC\/AS interaction in regenerative axon targeting. Anosmin-1 improved rat forepaw grasping as revealed by forelimb proprioception assessment. After treated with anosmin-1, p75+ immature SCs and P0+ mature SCs mingled well with ASs at the peripheral\/central glial interface, reforming the glial barrier from a tight to loose structure. Furthermore, regenerated axons traced by BDA staining revealed proper axonal targeting to the dorsal horn of the spinal cord. These results suggest that anosmin-1 can regulate SC\/AS interactions at the peripheral\/central boundary site to open the glial barrier for regenerating axons crossing, targeting, and establishing functional neuronal circuits. Anosmin-1 might have a potential application in repair of spinal cord injuries, particularly in combination with SCs for autologous cell transplantation.<\/jats:p>","DOI":"10.1177\/09636897251362107","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T10:53:37Z","timestamp":1754909617000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Extracellular matrix protein anosmin-1 regulates Schwann cell-astrocyte interaction for regenerative axon targeting in dorsal root crush injury model"],"prefix":"10.1177","volume":"34","author":[{"given":"Xichen","family":"Xuan","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuechun","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiliang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Chengdu Seventh People\u2019s Hospital (Affiliated Cancer Hospital of Chengdu Medical College), Chengdu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine, Jiangnan University Medical Center, Wuxi No.2 People\u2019s Hospital, Wuxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daqing","family":"Li","sequence":"additional","affiliation":[{"name":"Spinal Repair Unit, Department of Brain Repair and Rehabilitation, University College London Institute of Neurology, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre Marc","family":"Bouloux","sequence":"additional","affiliation":[{"name":"Centre for Neuroendocrinology, Royal Free Campus, University College Medical School, University College London, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8941-6920","authenticated-orcid":false,"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Spinal Repair Unit, Department of Brain Repair and Rehabilitation, University College London Institute of Neurology, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5507-6123","authenticated-orcid":false,"given":"Youli","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,8,11]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.382257"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells12060853"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2017"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23282"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-012-9740-6"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1136(200012)32:3<214::aid-glia20>3.0.co;2-7"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells10051186"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2006.01658.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2016.09.002"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23161"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.04.031"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(95)03518-c"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2010.01.004"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci43699"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.14483"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.12089\/jca.2021.05.017"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj20041078"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19914-3"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.1997.19010051.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.06.010"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1177\/0963689719855938"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.5.1009"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.660259"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.21150"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1097\/NEN.0b013e318257fe7b"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.3400-04.2004"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2012-1555"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-014-0977-4"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-014-0904-8"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2010.01.006"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.07.006"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2012.52"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells13070581"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201745294"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21093343"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells13131160"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00144"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-67526-0"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awp256"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.1184-07.2007"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20049"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.6340-11.2012"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.049155"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202400444"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.adi6412"}],"container-title":["Cell Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09636897251362107","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09636897251362107","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09636897251362107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:36:14Z","timestamp":1777390574000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09636897251362107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":45,"alternative-id":["10.1177\/09636897251362107"],"URL":"https:\/\/doi.org\/10.1177\/09636897251362107","relation":{},"ISSN":["0963-6897","1555-3892"],"issn-type":[{"value":"0963-6897","type":"print"},{"value":"1555-3892","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]},"article-number":"09636897251362107"}}