{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:01:02Z","timestamp":1777510862373,"version":"3.51.4"},"reference-count":44,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[1994,1,1]],"date-time":"1994-01-01T00:00:00Z","timestamp":757382400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:p>Varying doses of FK506, and a cell-depleting anti-CD4 monoclonal antibody, GK1.5, were tested as either monotherapy or in combination for their effect on the survival of renal subcapsular xenografts of organ-cultured fetal pig pancreas in three strains of mice. Subcutaneous injections of FK506 at 4.0 mg\/kg\/day for 28 d prevented graft rejection to day 35 posttransplantation (i.e., 7 days after cessation of treatment in NOD\/Lt, and CBA mice) while BALB\/c mice had intact grafts at 28 days. Lower doses were less effective and immunosuppression was less effective in NOD mice than in the other strains. Even 2.0 mg\/kg\/day of FK506 prevented rejection in CBA mice until day 35, but not in NOD\/Lt mice. GK1.5 alone did not prevent rejection in NOD\/Lt mice but when a low dose of FK506 (2.0 mg\/day) was added, the grafts were present, essentially intact, at 35 days. There were no obvious toxic effects of FK506 in NOD\/Lt and CBA mice. With FK506 treatment there was no significant difference in absolute numbers of total leucocytes or lymphocytes in peripheral blood and spleen, but there was a decrease in thymus cellularity. Flow cytometric analysis of lymphocyte subsets in blood and spleen also showed no significant differences, but in the thymus the percentage of immature CD4\/CD8 \u201cdouble positive\u201d cells increased while the more mature CD3 \u201chigh \u201d, and CD4 or CD8 \u201csingle-positive\u201d cells decreased. Thus, prolonged discordant xenograft survival in mice is possible and the use of two agents that act on different parts of the immune system allows a reduction in the dose of FK506 to safe levels.<\/jats:p>","DOI":"10.1177\/096368979400300110","type":"journal-article","created":{"date-parts":[[2017,11,7]],"date-time":"2017-11-07T09:02:23Z","timestamp":1510045343000},"page":"61-73","source":"Crossref","is-referenced-by-count":9,"title":["Effect of FK506 and Anti-CD4 Therapy on Fetal Pig Pancreas Xenografts and Host Lymphoid Cells in NOD\/Lt, CBA, and BALB\/c Mice"],"prefix":"10.1177","volume":"3","author":[{"given":"Kazuhiro","family":"Akita","sequence":"first","affiliation":[{"name":"Transplantation Unit, The Walter and Eliza Hall Institute of Medical Research, Parkville, 3050, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Transplantation Unit, The Walter and Eliza Hall Institute of Medical Research, Parkville, 3050, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.E.","family":"Mandel","sequence":"additional","affiliation":[{"name":"Transplantation Unit, The Walter and Eliza Hall Institute of Medical Research, Parkville, 3050, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,7,7]]},"reference":[{"key":"bibr1-096368979400300110","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198807000-00001"},{"key":"bibr2-096368979400300110","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.4049\/jimmunol.148.4.1049","volume":"148","author":"Bishop D.K.","year":"1992","journal-title":"J. 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