{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:59:43Z","timestamp":1777510783881,"version":"3.51.4"},"reference-count":45,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[1994,1,1]],"date-time":"1994-01-01T00:00:00Z","timestamp":757382400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:p>The outcome of islet isolation is considered uncertain because of the large variability of islet and insulin yield, but comparison of the isolated and native islet population has not been attempted. We therefore addressed the efficacy of collagenase digestion, and density gradient purification of islets from the splenic dog pancreas (n = 31) by morphometry of the islet volume and size distribution, and by extraction of insulin and amylase, in samples from the pancreas, the digest, and gradient fractions. In contrast to a ~90% recovery of pancreatic insulin and amylase after digestion, islet yield amounted to 50% of the islet content of the pancreas. After density separation, islets were mainly found in the purified fractions, while half of the recovered insulin was located in the acinar fraction of the gradients \u2014 indicating a substantial proportion of islets entrapped in acinar fragments. The islet and insulin content of the pancreas correlated well with islet and insulin yield after digestion (r = 0.7, p &lt; .0001). The insulin content of digest suspensions did neither correlate with islet nor insulin recovery in the purified fraction of the gradients (r = 0.4) as opposed to the islet content of digest suspensions, which correlated with both (r = 0.7, p &lt; .0001). After density separation near 100% purity was obtained, and no loss of insulin from isolated islets was demonstrated by extraction and microscopy. Size distributions of native and isolated islets demonstrated no fragmentation. We conclude that the variability of isolation outcome may be attributed to a large extent to the variability of the native endocrine pancreas. Isolation efficacy was best documented by morphometry, because insulin extraction did not discriminate between free and entrapped islets. However, assessment by both morphometry and extraction allowed the quantitation of entrapped islets, and demonstrated preservation of \u03b2-cell granulation. Similar studies should facilitate the analysis of other factors affecting islet isolation in man.<\/jats:p>","DOI":"10.1177\/096368979400300113","type":"journal-article","created":{"date-parts":[[2017,11,7]],"date-time":"2017-11-07T09:02:23Z","timestamp":1510045343000},"page":"91-101","source":"Crossref","is-referenced-by-count":10,"title":["Assessment of Islet Isolation Efficacy in Dogs"],"prefix":"10.1177","volume":"3","author":[{"given":"Michael P.M.","family":"Van Der Burg","sequence":"first","affiliation":[{"name":"Departments of Surgery, University Hospital, PO Box 9600, Leiden NL2300 RC, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Onno R.","family":"Guicherit","sequence":"additional","affiliation":[{"name":"Departments of Surgery, University Hospital, PO Box 9600, Leiden NL2300 RC, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marijke","family":"Fr\u00f6lich","sequence":"additional","affiliation":[{"name":"Departments of Clinical Chemistry, University Hospital, PO Box 9600, Leiden NL2300 RC, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes P.","family":"Scherft","sequence":"additional","affiliation":[{"name":"Departments of Cell Biology and Histology, University Hospital, PO Box 9600, Leiden NL2300 RC, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frans A.","family":"Prins","sequence":"additional","affiliation":[{"name":"Departments of Pathology, University Hospital, PO Box 9600, Leiden NL2300 RC, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan Anthonie","family":"Bruijn","sequence":"additional","affiliation":[{"name":"Departments of Pathology, University Hospital, PO Box 9600, Leiden NL2300 RC, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hein G.","family":"Gooszen","sequence":"additional","affiliation":[{"name":"Departments of Surgery, University Hospital, PO Box 9600, Leiden NL2300 RC, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,7,7]]},"reference":[{"key":"bibr1-096368979400300113","first-page":"99","volume":"19","author":"Abri O.","year":"1987","journal-title":"Transplant. 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