{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:55:24Z","timestamp":1777510524957,"version":"3.51.4"},"reference-count":48,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[1997,3,1]],"date-time":"1997-03-01T00:00:00Z","timestamp":857174400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[1997,3]]},"abstract":"<jats:p>\n                    The cellular response of isolated hepatocytes from pigs, humans, and human hepatoblastoma cells to freezing was characterized using cryomicroscopy and analyzed using a thermodynamic model for water transport and Intracellular Ice Formation (IIF). The value for the reference permeability, L\n                    <jats:sub>pg<\/jats:sub>\n                    , was found to be 5.8(10)\n                    <jats:sup>-13<\/jats:sup>\n                    , 1.62(10)\n                    <jats:sup>13<\/jats:sup>\n                    , and 2.7(10)-\n                    <jats:sup>14<\/jats:sup>\n                    m\/Ns for pig, human, and Hep G2\/C3A cells, respectively. The activation energy, E\n                    <jats:sub>lp<\/jats:sub>\n                    , was found to be 480 kJ\/mol for pig hepatocytes, 216 kJ\/mol for human, and 121 kJ\/mol for Hep G2\/C3A cells. The average temperature at which IIF (T\n                    <jats:sup>avg<\/jats:sup>\n                    <jats:sub>IIF<\/jats:sub>\n                    ) occurs was calculated to be -7.24 + 2.3\u00b0C for pig hepatocytes, -8.5 + 2.6\u00b0C for human hepatocytes, and -9.6 + 4.5\u00b0C for Hep G2\/C3A cells. These results indicate that the freezing characteristics of pig and human cells are distinct and that the specific freezing characteristics need to be understood for the development of appropriate freezing protocols.\n                  <\/jats:p>","DOI":"10.1177\/096368979700600211","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T15:18:33Z","timestamp":1498144713000},"page":"173-183","source":"Crossref","is-referenced-by-count":9,"title":["Freezing Characteristics of Isolated Pig and Human Hepatocytes"],"prefix":"10.1177","volume":"6","author":[{"given":"T.B.","family":"Darr","sequence":"first","affiliation":[{"name":"Biomedical Engineering Center, University of Minnesota, Box 107 UMHC, Minneapolis, MN 55455 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Hubel","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Center, University of Minnesota, Box 107 UMHC, Minneapolis, MN 55455 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,6,22]]},"reference":[{"key":"bibr1-096368979700600211","first-page":"460","volume-title":"Laboratory techniques in biochemistry and molecular biology.","author":"Berry A.E.M.","year":"1991"},{"issue":"2","key":"bibr2-096368979700600211","first-page":"406","volume":"18","author":"Chesne C.","year":"1993","journal-title":"Hepatology"},{"key":"bibr3-096368979700600211","first-page":"17","volume":"10","author":"Cosman M.","year":"1989","journal-title":"Cryoletters"},{"key":"bibr4-096368979700600211","doi-asserted-by":"publisher","DOI":"10.1007\/BF02620991"},{"key":"bibr5-096368979700600211","first-page":"269","volume":"29","author":"Darr T.B.","year":"1995","journal-title":"Trans. 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