{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:55:35Z","timestamp":1777510535181,"version":"3.51.4"},"reference-count":35,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[1997,9,1]],"date-time":"1997-09-01T00:00:00Z","timestamp":873072000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>\n                    The provision of an immediate supply of isolated porcine hepatocytes for artificial liver support requires preservation techniques that will allow maintenance of cell viability and detoxification functions. By means of a simple and cost-effective cryopreservation system, porcine hepatocytes can be available for both local and distant medical treatment facilities. Additionally, cryopreservation provides an adequate period for quality control testing to be completed prior to use of any specific cell lot. We are reporting a dual approach, namely the preservation of porcine hepatocytes, at 4\u00b0C and at \u2212196\u00b0C in liquid nitrogen (LN\n                    <jats:sub>2<\/jats:sub>\n                    ). Using a combination of cryoprotectant agents with Chee's modified Eagle's culture media (CEM), collagenase isolated hepatocytes stored at 4\u00b0C for 24 h maintained 80% of the initial diazepam metabolism measured in freshly isolated cells and nearly 100% of initial function was preserved in hepatocytes stored up to 6 mo at -196\u00b0C. University of Wisconsin solution (UW) was also tested and while adequate for 4\u00b0C storage, it certainly did not match the performance of the CEM formulations for preservation of metabolic function of cells stored in liquid nitrogen. Based on our results of viability and detoxification function the combination of CEM with DMSO, polyethylene glycol and serum provided optimal protection for LN\n                    <jats:sub>2<\/jats:sub>\n                    frozen cells. Other findings in these studies underlined the importance of the gradual introduction of DMSO in the prefreezing process, the period of osmotic equilibration, and the rapid postthaw withdrawal of this agent to minimize cytotoxic effects at these critical stages. Our freezing methodology provides the foundation for further technological developments in the cryopreservation of the large numbers of cells (billions) that are necessary for extracorporeal liver assist devices.\n                  <\/jats:p>","DOI":"10.1177\/096368979700600503","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T15:19:57Z","timestamp":1498144797000},"page":"447-454","source":"Crossref","is-referenced-by-count":14,"title":["Functional Recovery of Porcine Hepatocytes after Hypothermic or Cryogenic Preservation for Liver Support Systems"],"prefix":"10.1177","volume":"6","author":[{"given":"Sharda","family":"Naik","sequence":"first","affiliation":[{"name":"Department of Pathology, Rhode Island Hospital, Providence, RI 02903"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry A.","family":"Santangini","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rhode Island Hospital, Providence, RI 02903"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donna M.","family":"Trenkler","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rhode Island Hospital, Providence, RI 02903"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudy J.P.","family":"Mullon","sequence":"additional","affiliation":[{"name":"Co.-Conn., Grace Biomedical, Lexington, MA 02173, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry A.","family":"Solomon","sequence":"additional","affiliation":[{"name":"Co.-Conn., Grace Biomedical, Lexington, MA 02173, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rhode Island Hospital, Providence, RI 02903"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugo O.","family":"Jauregui","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rhode Island Hospital, Providence, RI 02903"},{"name":"Department of Pathology and Laboratory Medicine, Division of Biology and Medicine, Brown University, Providence, RI 02903"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,6,22]]},"reference":[{"key":"bibr1-096368979700600503","doi-asserted-by":"publisher","DOI":"10.1177\/096368979500400607"},{"key":"bibr2-096368979700600503","first-page":"189","volume-title":"GABA and benzodiazepine receptor subtypes.","author":"Basile A.S.","year":"1990"},{"key":"bibr3-096368979700600503","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199508000-00002"},{"key":"bibr4-096368979700600503","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(84)90198-6"},{"key":"bibr5-096368979700600503","doi-asserted-by":"publisher","DOI":"10.1177\/096368979200100405"},{"key":"bibr6-096368979700600503","first-page":"406","volume":"18","author":"Chesn\u00e9 C.","year":"1993","journal-title":"Hepatology"},{"key":"bibr7-096368979700600503","first-page":"29","volume-title":"Methods in enzymology","author":"Coriell L.L.","year":"1979"},{"key":"bibr8-096368979700600503","doi-asserted-by":"publisher","DOI":"10.1016\/0011-2240(90)90028-3"},{"key":"bibr9-096368979700600503","doi-asserted-by":"publisher","DOI":"10.3109\/00365529509107771"},{"key":"bibr10-096368979700600503","doi-asserted-by":"publisher","DOI":"10.1006\/cryo.1993.1011"},{"key":"bibr11-096368979700600503","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199303000-00028"},{"key":"bibr12-096368979700600503","doi-asserted-by":"publisher","DOI":"10.1016\/0011-2240(92)90048-7"},{"key":"bibr13-096368979700600503","unstructured":"FreshneyR.I. 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