{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:55:55Z","timestamp":1777510555123,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[1997,9,1]],"date-time":"1997-09-01T00:00:00Z","timestamp":873072000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>\n                    Intracranial implantation of polymer-encapsulated PC-12 cells has been shown to improve motor behavioral performance in animal models of Parkinson's disease. The purpose of this blinded study was to examine whether such improvement is associated with the active uptake and metabolism of dopamine precursors by intracerebrally implanted polymer-encapsulated PC-12 cells. In an in vitro experiment we demonstrate that\n                    <jats:sup>3<\/jats:sup>\n                    H-dopamine uptake by PC-12 cells was 10\n                    <jats:sup>8<\/jats:sup>\n                    fmol\/min \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    cells, and that this uptake can be specifically blocked 88% by the addition of 10 nM of nomifensine. In the in vivo experiments, polymer-encapsulated PC-12 cells were implanted in four MPTP-treated monkeys into the left deep parietal white matter (R1) or left striatum (R2-4). A fifth MPTP-treated monkey (R5) served as a control and received left striatal implants of empty capsules.\n                    <jats:sup>18<\/jats:sup>\n                    F-Dopa Positron Emission Tomography (PET) imaging was performed on each monkey before and after implantation surgery by blinded investigators. PET images obtained 5-13 wk after implantation demonstrated well delineated focal areas of high\n                    <jats:sup>18<\/jats:sup>\n                    F-dopa uptake in R1, R2, and R4. The focal area of high\n                    <jats:sup>18<\/jats:sup>\n                    F-dopa uptake in R1 precisely coregistered on a brain magnetic resonance image to the site of implantation. R3 (in whom the polymer-encapsulated PC-12 cells demonstrated poor cell survival upon explantation) and R5 (empty capsules) failed to demonstrate any area of increased\n                    <jats:sup>18<\/jats:sup>\n                    F-dopa uptake in their PET images. Histological examination of the host brain revealed no sprouting of dopaminergic nerve terminals around the implantation sites of the polymer-encapsulated PC-12 cells. These results indicate that the previously noted behavioral improvement after intrastriatal implantation of polymer encapsulated PC-12 cells is at least in part due to their highly specific uptake and metabolism of dopamine precursors. Furthermore, these data suggest that polymer-encapsulated PC-12 cells can store, reuptake, and functionally replenish dopamine and therefore, may be an effective treatment for Parkinson's disease.\n                  <\/jats:p>","DOI":"10.1177\/096368979700600506","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T15:19:57Z","timestamp":1498144797000},"page":"469-477","source":"Crossref","is-referenced-by-count":17,"title":["Polymer-Encapsulated PC-12 Cells Demonstrate High-Affinity Uptake of Dopamine in Vitro and\n                    <sup>18<\/sup>\n                    F-DOPA Uptake and Metabolism after Intracerebral Implantation in Nonhuman Primates"],"prefix":"10.1177","volume":"6","author":[{"given":"Thyagarajan","family":"Subramanian","sequence":"first","affiliation":[{"name":"Departments of Neurology and Emory University School of Medicine"},{"name":"Yerkes Primate Research Center and PET Center, Atlanta, GA 30322, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dwaine F.","family":"Emerich","sequence":"additional","affiliation":[{"name":"CytoTherapeutics, Inc., Providence, RI 02906, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy A. E.","family":"Bakay","sequence":"additional","affiliation":[{"name":"Neurosurgery, Emory University School of Medicine"},{"name":"Yerkes Primate Research Center and PET Center, Atlanta, GA 30322, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John M.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Emory University School of Medicine"},{"name":"Emory PET Center, Atlanta, GA 30322, USA Inc., Providence, RI 02906, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark M.","family":"Goodman","sequence":"additional","affiliation":[{"name":"Emory PET Center, Atlanta, GA 30322, USA Inc., Providence, RI 02906, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy M.","family":"Shoup","sequence":"additional","affiliation":[{"name":"Emory PET Center, Atlanta, GA 30322, USA Inc., Providence, RI 02906, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary W.","family":"Miller","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Emory University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan I.","family":"Levey","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Emory University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George W.","family":"Hubert","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Emory University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Batchelor","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Emory University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelly R.","family":"Winn","sequence":"additional","affiliation":[{"name":"CytoTherapeutics, Inc., Providence, RI 02906, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel A.","family":"Saydoff","sequence":"additional","affiliation":[{"name":"CytoTherapeutics, Inc., Providence, RI 02906, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ray L.","family":"Watts","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Emory University School of Medicine"},{"name":"Yerkes Primate Research Center and PET Center, Atlanta, GA 30322, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,6,22]]},"reference":[{"key":"bibr1-096368979700600506","first-page":"1275","volume":"58","author":"Aebischer P.","year":"1994","journal-title":"A preliminary report. 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