{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T02:42:28Z","timestamp":1778899348822,"version":"3.51.4"},"reference-count":49,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[1997,11,1]],"date-time":"1997-11-01T00:00:00Z","timestamp":878342400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[1997,11]]},"abstract":"<jats:p>The study examined the importance of embryonic donor age for the survival of nigral grafts in 6-OHDA\u2013lesioned marmosets. The issue as to whether donor age is critical for the survival of nigral grafts in primates is controversial, because several early reports suggested that relatively old tissue could survive transplantation and produce functional benefits in monkeys, in contrast to the restrictive time dependence observed in rodents. Embryonic marmoset donors embryos of three different ages were employed: 1) E74 (Carnegie stage 18-19); 2) E83-84 (Carnegie stage 23+); 3) E92-93 (foetal period). The nigral neurons derived from the ventral mesencephalon in the two older donor age groups did not survive well when grafted to the striatum of adult marmosets with unilateral 6-OHDA lesions. Although a few tyrosine hydroxylase (TH+) neurons could be identified by immunohistochemistry at graft sites in all recipients in older donor age groups, the numbers of surviving neurons in these were small, on average typically less than 100 TH+ cells. These small grafts were not sufficient to affect amphetamine-induced rotation. In contrast, many more TH+ cells typically survived transplantation in the recipients; of graft tissue derived from the youngest donors and amphetamine-induced rotation was significantly reduced in this group alone. The time course and extent of the reduction in rotation was remarkably similar to that observed in previous marmoset nigral graft studies, confirming the utility of amphetamine-induced rotation as a sensitive and reliable indicator of nigral graft function in this species. Considering these results and other recent evidence from monkey to monkey, human to rat, and human to human graft studies, the survival of embryonic nigral tissues derived from primate donors transplanted into the striatum does appear to be critically dependent on the age of the donor tissue.<\/jats:p>","DOI":"10.1177\/096368979700600606","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T15:17:38Z","timestamp":1498144658000},"page":"557-569","source":"Crossref","is-referenced-by-count":20,"title":["Survival of Nigral Grafts within the Striatum of Marmosets with 6-Ohda Lesions Depends Critically on Donor Embryo Age"],"prefix":"10.1177","volume":"6","author":[{"given":"L. E.","family":"Annett","sequence":"first","affiliation":[{"name":"Department of Experimental Psychology, University of Cambridge, Cambridge CB2 3EB and The MRC Cambridge Centre for Brain Repair, Cambridge CB2 2PY"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. M.","family":"Torres","sequence":"additional","affiliation":[{"name":"Department of Experimental Psychology, University of Cambridge, Cambridge CB2 3EB and The MRC Cambridge Centre for Brain Repair, Cambridge CB2 2PY"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. J.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Anatomy, University of Oxford, Oxford OX1 3QX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Ishida","sequence":"additional","affiliation":[{"name":"Department of Anatomy, University of Oxford, Oxford OX1 3QX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. A.","family":"Barker","sequence":"additional","affiliation":[{"name":"Department of Experimental Psychology, University of Cambridge, Cambridge CB2 3EB and The MRC Cambridge Centre for Brain Repair, Cambridge CB2 2PY"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M.","family":"Ridley","sequence":"additional","affiliation":[{"name":"Department of Experimental Psychology, University of Cambridge, Cambridge CB2 3EB and The MRC Cambridge Centre for Brain Repair, Cambridge CB2 2PY"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. F.","family":"Baker","sequence":"additional","affiliation":[{"name":"Department of Experimental Psychology, University of Cambridge, Cambridge CB2 3EB and The MRC Cambridge Centre for Brain Repair, Cambridge CB2 2PY"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. B.","family":"Dunnett","sequence":"additional","affiliation":[{"name":"Department of Experimental Psychology, University of Cambridge, Cambridge CB2 3EB and The MRC Cambridge Centre for Brain Repair, Cambridge CB2 2PY"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1997,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1090940210"},{"key":"e_1_2_1_3_2","first-page":"71","volume-title":"Functional neural transplantation.","author":"Annett L.E.","year":"1994","unstructured":"AnnettL.E. Functional studies of neural grafts in parkinsonian primates. In: DunnettS.B. Bj\u00f6rklundA. eds. Functional neural transplantation. New York: Raven Press; 1994: 71\u2013102."},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1994.1026"},{"key":"e_1_2_1_5_2","first-page":"123","volume-title":"Neural Transplantation: A practical approach.","author":"Annett L.E.","year":"1992","unstructured":"AnnettL.E. RidleyR.M. Neural transplantation in primates. In: DunnettS.B. Bj\u00f6rklundA. eds. Neural Transplantation: A practical approach. Oxford: IRL Press; 1992: 123\u2013138."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/115.3.825"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/BF00241495","article-title":"A comparison of the behavioural effects of embryonic nigral grafts in the caudate nucleus and in the putamen of marmosets with unilateral 6-OHDA lesions.","volume":"103","author":"Annett L.E.","year":"1995","unstructured":"AnnettL.E. TorresE.M. RidleyR.M. BakerH.F. DunnettS.B. A comparison of the behavioural effects of embryonic nigral grafts in the caudate nucleus and in the putamen of marmosets with unilateral 6-OHDA lesions. Exp. Brain Res. 103: 355\u2013371; 1995.","journal-title":"Exp. Brain Res."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1991.74.1.0097"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/096368979500400204"},{"key":"e_1_2_1_10_2","first-page":"47","volume-title":"Functional neural transplantation.","author":"Bj\u00f6rklund A.","year":"1994","unstructured":"Bj\u00f6rklundA. DunnettS.B. NikkahG. Nigral transplants in the rat Parkinson model: Functional limitations and strategies to enhance nigro-striatal reconstruction. In: DunnettS.B. Bj\u00f6rklundA. eds. Functional neural transplantation. New York: Raven Press; 1994: 47\u201369."},{"key":"e_1_2_1_11_2","first-page":"139","volume-title":"Neural transplantation: A practical approach.","author":"Brundin P.","year":"1992","unstructured":"BrundinP. Dissection, preparation, and implantation of human embryonic brain tissue. In: DunnettS.B. Bj\u00f6rklundA. eds. Neural transplantation: A practical approach. Oxford: IRL Press; 1992: 139\u2013160."},{"key":"e_1_2_1_12_2","first-page":"9","volume-title":"Functional neural transplantation.","author":"Brundin P.","year":"1994","unstructured":"BrundinP. DuanW.-M. SauerH. Functional effects of mesencephalic dopamine neurons and adrenal chromaffin cells grafted to the rodent striatum. In: DunnettS.B. Bj\u00f6rklundA. ed. Functional neural transplantation. New York: Raven Press; 1994: 9\u201346."},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00243848"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00271860"},{"key":"e_1_2_1_15_2","volume-title":"An atlas for staging mammalian and chick embryos.","author":"Butler H.","year":"1987","unstructured":"ButlerH. JuurlinkB.H.J. An atlas for staging mammalian and chick embryos. Boca Raton, FL: CRC Press, Inc.; 1987."},{"key":"e_1_2_1_16_2","first-page":"27","volume-title":"Intracerebral transplantation in movement disorders.","author":"Dunnett S.B.","year":"1991","unstructured":"DunnettS.B. AnnettL.E. Nigral transplants in primate models of parkinsonism. In: LindvallO. Bj\u00f6rklundA. WidnerH. eds. Intracerebral transplantation in movement disorders. Amsterdam: Elsevier; 1991: 27\u201351."},{"key":"e_1_2_1_17_2","first-page":"1","volume-title":"Neural transplantation: A practical approach.","author":"Dunnett S.B.","year":"1992","unstructured":"DunnettS.B. Bj\u00f6rklundA. Staging and dissection of rat embryos. In: DunnettS.B. Bj\u00f6rklundA. eds. Neural transplantation: A practical approach. Oxford: IRL Press; 1992: 1\u201319."},{"key":"e_1_2_1_18_2","volume-title":"Functional neural transplantation.","author":"Dunnett S.B.","year":"1994","unstructured":"DunnettS.B. Bj\u00f6rklundA. Functional neural transplantation. New York: Raven Press; 1994."},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00247501"},{"key":"e_1_2_1_20_2","first-page":"161","volume-title":"Function and dysfunction in the basal ganglia.","author":"Elsworth J.D.","year":"1990","unstructured":"ElsworthJ.D. RedmondD.E.Jr. SladekJ.R.Jr. DeutchA.Y. CollierT.J. RothR.H. Reversal of MPTP-induced Parkinsonism in primates by fetal dopamine cell transplants. In: FranksA.J. IronsideJ.W. MindhamR.H.S. SmithR.J. SpokesE.G.S. WinlowW. eds. Function and dysfunction in the basal ganglia. Manchester: Manchester University Press; 1990: 161\u2013180."},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(95)00564-1"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/978-1-349-10047-7_61","volume-title":"Pharmacology and functional regulation of dopaminergic neurons.","author":"Freed C.R.","year":"1988","unstructured":"FreedC.R. RichardsJ.B. SabolK.E. ReiteM.L. Fetal substantia nigra transplants lead to dopamine cell replacement and behavioural improvement in Bonnet monkeys with MPTP induced parkinsonism. In: BeartP.M. WoodruffG.N. JacksonD.M. eds. Pharmacology and functional regulation of dopaminergic neurons. London: Macmillan; 1988: 353\u2013360."},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410380307"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1177\/096368979500400118"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(91)90025-8"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1991.00530200062020"},{"key":"e_1_2_1_27_2","first-page":"305","volume-title":"Breeding simians for developmental biology, vol 6.","author":"Hendrickx A.G.","year":"1975","unstructured":"HendrickxA.G. SawyerR.H. LasleyB.L. BarnesR.D. Comparison of developmental stages in primates with a note on the detection of ovulation. In: PerkinsP.T. O'DonoghueP.N. eds. Breeding simians for developmental biology, vol 6. London: Laboratory Animal Handbooks. 1975: 305\u2013315."},{"key":"e_1_2_1_28_2","first-page":"79","volume-title":"Intracerebral transplantation in movement disorders.","author":"Hitchcock E.H.","year":"1991","unstructured":"HitchcockE.H. HendersonB.T.H. KennyB.G. CloughC.G. HughesR.C. DettaA. Stereotactic implantation of foetal mesencephalon. In: LindvallO. Bj\u00f6rklundA. WidnerH. eds. Intracerebral transplantation in movement disorders. Amsterdam: Elsevier; 1991: 79\u201386."},{"key":"e_1_2_1_29_2","first-page":"3","article-title":"Survival of TH-positive and neuromelanin-containing cells in patients with Parkinson's disease after intrastriatal grafting of fetal ventral mesencephalon.","volume":"129","author":"Hitchcock E.H.","year":"1994","unstructured":"HitchcockE.H. WhitwellH.L. SofroniewM.V. BankiewiczK.S. Survival of TH-positive and neuromelanin-containing cells in patients with Parkinson's disease after intrastriatal grafting of fetal ventral mesencephalon. Exp. Neurol. 129: 3; 1994.","journal-title":"Exp. Neurol."},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1177\/096368979600500221"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199504273321702"},{"key":"e_1_2_1_32_2","first-page":"103","volume-title":"Functional neural transplantation.","author":"Lindvall O.","year":"1994","unstructured":"LindvallO. Neural transplantation in Parkinson's disease. In: DunnettS.B. Bj\u00f6rklundA. eds. Functional neural transplantation. New York: Raven Press; 1994: 103\u2013137."},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410350208"},{"key":"e_1_2_1_34_2","doi-asserted-by":"crossref","first-page":"12408","DOI":"10.1073\/pnas.91.26.12408","article-title":"Lazaroids improve the survival of grafted rate embryonic dopamine neurons.","volume":"91","author":"Nakao N.","year":"1994","unstructured":"NakaoN. FrodlE.M. DuanW.-M. WidnerH. BrundinP. Lazaroids improve the survival of grafted rate embryonic dopamine neurons. Proc. Natl. Acad. Sci. USA 91: 12408\u201312412; 1994.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0395-226"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(96)80038-5"},{"key":"e_1_2_1_37_2","volume-title":"Developmental stages in human embryos.","author":"O'Rahilly R.","year":"1987","unstructured":"O'RahillyR. M\u00fcllerF. Developmental stages in human embryos. Washington: Carnegie Institute; 1987."},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)93294-Y"},{"key":"e_1_2_1_39_2","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1016\/S0140-6736(86)91839-8","article-title":"Fetal neuronal grafts in monkeys given methylphenylterahydropyridine.","volume":"8490","author":"Redmond D.E.","year":"1986","unstructured":"RedmondD.E.Jr. SladekJ.R.Jr. RothR.H. CollierT.J. ElsworthJ.D. DeutchA.Y. HaberS. Fetal neuronal grafts in monkeys given methylphenylterahydropyridine. Lancet 8490: 1125\u20131127; 1986.","journal-title":"Lancet"},{"key":"e_1_2_1_40_2","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1002\/ana.410380406","article-title":"Clinical correlates of [18F]Fluorodopa uptake in five grafted Parkinsonian patients.","volume":"38","author":"Remy P.","year":"1995","unstructured":"RemyP. SamsonY. HantrayeP. FontaineA. DeferG. ManginJ.-F. F\u00e9nelonG. G\u00e9nyC. RicolfiF. FrouinV. N'GuyenJ.-P. JenyR. DegosJ.-D. PeschanskiM. CesaroP. Clinical correlates of [18F]Fluorodopa uptake in five grafted Parkinsonian patients. Ann. Neurol. 38: 1580\u2013588; 1995.","journal-title":"Ann. Neurol."},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410310207"},{"key":"e_1_2_1_42_2","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/BF00221102","article-title":"Quantitation of fiber growth in transplanted central monoamine neurons.","volume":"179","author":"Seiger A.","year":"1977","unstructured":"SeigerA. OlsonL. Quantitation of fiber growth in transplanted central monoamine neurons. Cell Tissue Res. 179: 285\u2013316; 1977.","journal-title":"Cell Tissue Res."},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199611040-00029"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(86)90092-4"},{"key":"e_1_2_1_45_2","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0079-6123(08)60323-4","article-title":"Fetal dopamine neural grafts: Extended reversal of methylphenyltetrahy-dropyridine-induced parkinsonism in monkeys.","volume":"78","author":"Sladek J.R.","year":"1988","unstructured":"SladekJ.R.Jr. RedmondD.E. CollierT.J. BlountJ.P. ElsworthJ.D. TaylorJ.R. RothR.H. Fetal dopamine neural grafts: Extended reversal of methylphenyltetrahy-dropyridine-induced parkinsonism in monkeys. Prog. Brain Res. 78: 497\u2013506; 1988.","journal-title":"Prog. Brain Res."},{"key":"e_1_2_1_46_2","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1006\/exnr.1993.1103","article-title":"Fetal dopamine cell survival after transplantation is dramatically improved at a critical donor age in nonhuman primates.","volume":"122","author":"Sladek J.R.","year":"1993","unstructured":"SladekJ.R.Jr. ElsworthJ.D. RothR.H. EvansL.E. CollierT.J. CooperS.J. TaylorJ.R. RedmondD.E. Fetal dopamine cell survival after transplantation is dramatically improved at a critical donor age in nonhuman primates. Exp. Neurol. 122: 16\u201327; 1993.","journal-title":"Exp. Neurol."},{"key":"e_1_2_1_47_2","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/BF00229411","article-title":"Grafting of fetal substantia nigra to striatum reverses behavioural deficits induced by MPTP primates: A comparison with other types of grafts as controls.","volume":"85","author":"Taylor J.R.","year":"1991","unstructured":"TaylorJ.R. ElsworthJ.D. RothR.H. SladekJ.R.Jr. CollierT.J. RedmondD.E.Jr. Grafting of fetal substantia nigra to striatum reverses behavioural deficits induced by MPTP primates: A comparison with other types of grafts as controls. Exp. Brain Res. 85: 335\u2013348; 1991.","journal-title":"Exp. Brain Res."},{"key":"e_1_2_1_48_2","doi-asserted-by":"publisher","DOI":"10.1177\/096368979500400105"},{"key":"e_1_2_1_49_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199211263272203"},{"key":"e_1_2_1_50_2","volume-title":"Statistical principles in experimental design.","author":"Winer B.J.","year":"1974","unstructured":"WinerB.J. Statistical principles in experimental design. New York: McGraw Hill; 1974."}],"container-title":["Cell Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/096368979700600606","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/096368979700600606","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:37:33Z","timestamp":1777390653000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/096368979700600606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,11]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1997,11]]}},"alternative-id":["10.1177\/096368979700600606"],"URL":"https:\/\/doi.org\/10.1177\/096368979700600606","relation":{},"ISSN":["0963-6897","1555-3892"],"issn-type":[{"value":"0963-6897","type":"print"},{"value":"1555-3892","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,11]]}}}