{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:51:41Z","timestamp":1777380701430,"version":"3.51.4"},"reference-count":35,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2020,8,2]],"date-time":"2020-08-02T00:00:00Z","timestamp":1596326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Acupunct Med"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Lipopolysaccharide (LPS) endotoxins are activators of innate immunity inducing infection and inflammatory responses. Anti-inflammatory drugs can have undesirable side effects. Acupuncture may be an alternative for the treatment of inflammatory processes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>We investigated the potential anti-inflammatory effect of manual acupuncture (MA) at SP6 upon LPS-induced peritonitis in rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Peritonitis was induced in rats with an intraperitoneal injection of LPS (0.002, 0.02, 0.2 or 2\u2009\u00b5g\/kg) in four experimental groups (n\u2009=\u20096 each). A fifth group was injected with sterile saline solution (saline group, n\u2009=\u20096). Four hours after the procedure, peritoneal fluid was collected to determine total cell counts for inflammatory cells, differential leukocyte counts and peritoneal capillary permeability. The LPS dose of 0.02\u2009\u00b5g\/kg was used in the subsequent experiments as it most successfully induced peritoneal inflammation. Subsequently, five experimental groups (n\u2009=\u200912 rats each) were used: (1) saline, (2) control (untreated LPS group), (3) indomethacin (LPS group treated with indomethacin), (4) NA (LPS group treated with MA at a location not corresponding to any traditional acupuncture point), and (5) SP6 (LPS group treated with verum MA at SP6). Ten minutes after MA or 30\u2009min after indomethacin treatment, the rats received an intraperitoneal injection of LPS. After 4\u2009h, total leukocyte and differential cell counts, myeloperoxidase (MPO) activity, vascular permeability and cytokine levels were evaluated in the peritoneal fluid. Cytokine levels were additionally evaluated in the brainstem.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>SP6 MA and indomethacin treatments reduced inflammatory cell infiltration, vascular permeability and MPO activity in the LPS-exposed rats. Pre-treatment with indomethacin and SP6 MA decreased tumor necrosis factor (TNF)-\u03b1 levels and preserved interleukin (IL)-10 in the peritoneal fluid. Indomethacin also reduced IL-6 in the peritoneal fluid. In the brainstem, indomethacin reduced IL-1\u03b2, IL-6, TNF\u03b1 and IL-10, whereas SP6 MA reduced only TNF\u03b1 and IL-6 levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>This study clearly demonstrates the anti-inflammatory effect of acupuncture, which we believe may involve the activation of anti-inflammatory neural reflexes in the regulation of peritonitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0964528420938379","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T01:02:51Z","timestamp":1596416571000},"page":"376-384","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Acupuncture reduces peripheral and brainstem cytokines in rats subjected to lipopolysaccharide-induced inflammation"],"prefix":"10.1177","volume":"39","author":[{"given":"Caroline C","family":"Ramires","sequence":"first","affiliation":[{"name":"Multicentric Post-graduation Program in Physiological Sciences, Federal University of Pampa, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela TL","family":"Balbinot","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Center for Biological Sciences, Laboratory of Neurobiology of Pain and Inflammation, Federal University of Santa Catarina, Florian\u00f3polis, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco J","family":"Cidral-Filho","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Center for Biological Sciences, Laboratory of Neurobiology of Pain and Inflammation, Federal University of Santa Catarina, Florian\u00f3polis, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Ventura","family":"Dias","sequence":"additional","affiliation":[{"name":"Laboratory of Anatomy, Federal University of Pampa, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adair RS","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Center for Biological Sciences, Laboratory of Neurobiology of Pain and Inflammation, Federal University of Santa Catarina, Florian\u00f3polis, Brazil"},{"name":"Neurosciences Post-Graduation Program, Center of Biological Sciences, Federal University of Santa Catarina, Florian\u00f3polis, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morgana Duarte","family":"da Silva","sequence":"additional","affiliation":[{"name":"Multicentric Post-graduation Program in Physiological Sciences, Federal University of Pampa, Rio Grande do Sul, Brazil"},{"name":"Department of Physiological Sciences, Center for Biological Sciences, Laboratory of Neurobiology of Pain and Inflammation, Federal University of Santa Catarina, Florian\u00f3polis, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,8,2]]},"reference":[{"key":"bibr1-0964528420938379","doi-asserted-by":"publisher","DOI":"10.1177\/09680519010070030101"},{"key":"bibr2-0964528420938379","first-page":"903","volume":"56","author":"Pardon MC.","year":"2015","journal-title":"Rom J Morphol 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