{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:54:06Z","timestamp":1777380846186,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2022,1,16]],"date-time":"2022-01-16T00:00:00Z","timestamp":1642291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81774394"],"award-info":[{"award-number":["81774394"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81804167"],"award-info":[{"award-number":["81804167"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Acupunct Med"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background and aim:<\/jats:title>\n                    <jats:p>Disordered hepatic energy metabolism is found in obese rats with insulin resistance (IR). There are insufficient experimental studies of electroacupuncture (EA) for IR and type 2 diabetes mellitus (T2DM). The aim of this study was to probe the effect of EA on disordered hepatic energy metabolism and the adenosine monophosphate (AMP)-activated protein kinase (AMPK)\/mammalian target of rapamycin complex 1 (mTORC1)\/ribosomal protein S6 kinase, 70-kDa (p70S6K) signaling pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Zucker Diabetic Fatty (ZDF) rats were randomly divided into three groups: EA group receiving EA treatment; Pi group receiving pioglitazone gavage; and ZF group remaining untreated (n\u2009=\u20098 per group). Inbred non-insulin-resistant Zucker lean rats formed an (untreated) healthy control group (ZL, n\u2009=\u20098). Fasting plasma glucose (FPG), fasting insulin (FINS), C-peptide, C-reactive protein (CRP) and homeostatic model assessment of insulin resistance (HOMA-IR) indices were measured. Hematoxylin\u2013eosin (H&amp;E) staining was used to investigate the liver morphologically. The mitochondrial structure of hepatocytes was observed by transmission electron microscopy (TEM). Western blotting was adopted to determine protein expression of insulin receptor substrate 1 (IRS-1), mTOR, mTORC1, AMPK, tuberous sclerosis 2 (TSC2) and p70S6K, and their phosphorylation. RT-PCR was used to quantify IRS-1, mTOR, mTORC1, AMPK and p70S6K mRNA levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Compared with the ZF group, FPG, FINS, C-peptide, CRP and HOMA-IR levels were significantly reduced in the EA group ( p\u2009&lt;\u20090.05, p\u2009&lt;\u20090.01). Evaluation of histopathology showed improvement in liver appearances following EA. Phosphorylation levels of AMPK, mTOR and TSC2 decreased, and IRS-1 and p70S6K increased, in hepatocytes of the ZF group, while these negative effects appeared to be alleviated by EA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>EA can effectively ameliorate IR and regulate energy metabolism in the ZDF rat model. AMPK\/mTORC1\/p70S6K and related molecules may represent a potential mechanism of action underlying these effects.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09645284211070301","type":"journal-article","created":{"date-parts":[[2022,1,16]],"date-time":"2022-01-16T23:25:25Z","timestamp":1642375525000},"page":"360-368","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Low-frequency electroacupuncture improves disordered hepatic energy metabolism in insulin-resistant Zucker diabetic fatty rats via the AMPK\/mTORC1\/p70S6K signaling pathway"],"prefix":"10.1177","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9321-0784","authenticated-orcid":false,"given":"Xiao-xiao","family":"Liu","sequence":"first","affiliation":[{"name":"Fourth Affiliated Hospital of Guangzhou University of Chinese Medicine, Shenzhen, China"},{"name":"South China Research Center for Acupuncture and Moxibustion, Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9027-3368","authenticated-orcid":false,"given":"Li-zhi","family":"Zhang","sequence":"additional","affiliation":[{"name":"South China Research Center for Acupuncture and Moxibustion, Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Tuina, Fourth Affiliated Hospital of Guangzhou University of Chinese Medicine, Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8980-2568","authenticated-orcid":false,"given":"Lan-feng","family":"Lai","sequence":"additional","affiliation":[{"name":"South China Research Center for Acupuncture and Moxibustion, Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9218-588X","authenticated-orcid":false,"given":"Yi-qiao","family":"Wang","sequence":"additional","affiliation":[{"name":"South China Research Center for Acupuncture and Moxibustion, Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Sun","sequence":"additional","affiliation":[{"name":"Traditional Therapy Department of Fangchun, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neng-gui","family":"Xu","sequence":"additional","affiliation":[{"name":"South China Research Center for Acupuncture and Moxibustion, Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-xing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Soft Tissue Traumatology, Fourth Affiliated Hospital of Guangzhou University of Chinese Medicine, Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,1,16]]},"reference":[{"key":"bibr1-09645284211070301","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-013-0433-5"},{"key":"bibr2-09645284211070301","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2017.151"},{"key":"bibr3-09645284211070301","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2019.107843"},{"key":"bibr4-09645284211070301","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0216172"},{"issue":"1","key":"bibr5-09645284211070301","first-page":"1","volume":"3","author":"Roberts 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