{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T14:56:43Z","timestamp":1793199403850,"version":"4.5.0"},"reference-count":41,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T00:00:00Z","timestamp":1697328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"This Project funded by Shanghai Jinshan District Health and Family Planning Commission","award":["JSKJ-KTZY-2019-01"],"award-info":[{"award-number":["JSKJ-KTZY-2019-01"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Acupunct Med"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>This study aimed to investigate the effects of electroacupuncture (EA) on cognitive recovery and synaptic remodeling in a rat model of middle cerebral artery occlusion (MCAO) followed by reperfusion and explore the possible mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method:<\/jats:title>\n                    <jats:p>Focal cerebral ischemia was modeled in healthy adult Sprague-Dawley rats by MCAO. The MCAO rats were classified into four groups: sham, MCAO, MCAO\u2009+\u2009GB20 (receiving EA at GB20) and MCAO\u2009+\u2009NA (receiving EA at a \u201cnon-acupoint\u201d location not corresponding to any traditional acupuncture point location about 10\u2009mm above the iliac crest). Neurological deficit scores and behavior were assessed before and during the treatment. After intervention for 7\u2009days, the hippocampus was dissected to analyze growth-associated protein (GAP)-43, synaptophysin (SYN) and postsynaptic density protein (PSD)-95 expression levels by Western blotting. Bioinformatic analysis and primary hippocampal neurons with calcium-voltage gated channel subunit alpha 1B (CACNA1B) gene overexpression were used to screen the target genes for EA against MCAO.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Significant amelioration of neurological deficits and learning\/memory were found in MCAO\u2009+\u2009GB20 rats compared with MCAO or MCAO\u2009+\u2009NA rats. Protein levels of GAP-43, SYN and PSD-95 were significantly improved in MCAO\u2009+\u2009GB20\u2013treated rats together with an increase in the number of synapses in the hippocampal CA1 region. CACNA1B appeared to be a target gene of EA in MCAO. There were increased mRNA levels of CACNA1B, calmodulin (CaM), Ca\n                      <jats:sup>2+<\/jats:sup>\n                      \/calmodulin-dependent protein kinase type II (CaMKII) and cyclic adenosine monophosphate response element binding (CREB) and increased phosphorylation of CaM, CaMKII and CREB in the hippocampal region in MCAO\u2009+\u2009GB20 versus MCAO and MCAO\u2009+\u2009NA groups. CACNA1B overexpression modulated expression of the CaM\u2013CaMKII\u2013CREB axis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>EA treatment at GB20 may ameliorate the negative effects of MCAO on cognitive function in rats by enhancing synaptic plasticity. EA treatment at GB20 may exert this neuroprotective effect by regulating the CACNA1B\u2013CaM\u2013CaMKII\u2013CREB axis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09645284231202805","type":"journal-article","created":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:31:30Z","timestamp":1697416290000},"page":"23-31","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":32,"title":["Electroacupuncture at GB20 improves cognitive ability and synaptic plasticity via the CaM\u2013CaMKII\u2013CREB signaling pathway following cerebral ischemia\u2013reperfusion injury in rats"],"prefix":"10.1177","volume":"42","author":[{"given":"Qing","family":"Han","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Medicine, Jinshan Hospital of Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Jinshan Hospital of Fudan University, Shanghai, 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