{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:55:51Z","timestamp":1777380951457,"version":"3.51.4"},"reference-count":43,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T00:00:00Z","timestamp":1714694400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Acupunct Med"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Cervical spondylosis (CS) is a prevalent disorder that can have a major negative impact on quality of life. Traditional conservative treatment has limited efficacy, and electroacupuncture (EA) is a novel treatment option. We investigated the application and molecular mechanism of EA treatment in a rat model of cervical intervertebral disk degeneration (CIDD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>The CIDD rat model was established, following which rats in the electroacupuncture (EA) group received EA. For overexpression of IL-22 or inhibition of JAK2-STAT3 signaling, the rats were injected intraperitoneally with recombinant IL-22 protein (p-IL-22) or the JAK2-STAT3 (Janus kinase 2\u2014signal transducer and activator of transcription protein 3) inhibitor AG490 after model establishment. Rat nucleus pulposus (NP) cells were isolated and cultured. Cell counting kit-8 and flow cytometry were used to analyze the viability and apoptosis of the NP cells. Expression of IL-22, JAK2 and STAT3 was determined using RT-qPCR. Expression of IL-22\/JAK2-STAT3 pathway and apoptosis related proteins was detected by Western blotting (WB).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>EA protected the NP tissues of CIDD rats by regulating the IL-22\/JAK2-STAT3 pathway. Overexpression of IL-22 significantly promoted the expression of tumor necrosis factor (TNF)-\u03b1, IL-6, IL-1\u03b2, matrix metalloproteinase (MMP)3 and MMP13 compared with the EA group. WB demonstrated that the expression of IL-22, p-JAK2, p-STAT3, caspase-3 and Bax in NP cells of the EA group was significantly reduced and Bcl-2 elevated compared with the model group. EA regulated cytokines and MMP through activation of IL-22\/JAK2-STAT3 signaling in CIDD rat NP cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>We demonstrated that EA affected apoptosis by regulating the IL-22\/JAK2-STAT3 pathway in NP cells and reducing inflammatory factors in the CIDD rat model. The results extend our knowledge of the mechanisms of action underlying the effects of EA as a potential treatment approach for CS in clinical practice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09645284241248465","type":"journal-article","created":{"date-parts":[[2024,5,4]],"date-time":"2024-05-04T00:20:26Z","timestamp":1714782026000},"page":"146-154","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Electroacupuncture improves apoptosis of nucleus pulposus cells via the IL-22\/JAK2-STAT3 signaling pathway in a rat model of cervical intervertebral disk degeneration"],"prefix":"10.1177","volume":"42","author":[{"given":"Sen","family":"Yan","sequence":"first","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling-Yao","family":"Xie","sequence":"additional","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia-Xia","family":"Duan","sequence":"additional","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia-Xuan","family":"Tan","sequence":"additional","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Yang","sequence":"additional","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Meng","sequence":"additional","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-Hua","family":"Zhong","sequence":"additional","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Di","family":"Lin","sequence":"additional","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia-Ni","family":"Yang","sequence":"additional","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao-Yao","family":"Xiao","sequence":"additional","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,5,3]]},"reference":[{"key":"bibr1-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1227\/01.NEU.0000215430.86569.C4"},{"key":"bibr2-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/493605"},{"key":"bibr3-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-018-2234-0"},{"key":"bibr4-09645284241248465","first-page":"47","volume":"11","author":"Wang C","year":"2016","journal-title":"Clin Interv Aging"},{"key":"bibr5-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1227\/01.NEU.0000166664.19662.43"},{"key":"bibr6-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/393642"},{"key":"bibr7-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32279-7"},{"key":"bibr8-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-013-2658-z"},{"key":"bibr9-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000018851"},{"key":"bibr10-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X17500859"},{"key":"bibr11-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1016\/S0254-6272(15)30083-2"},{"key":"bibr12-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2009.07.002"},{"key":"bibr13-09645284241248465","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21103601"},{"key":"bibr14-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1978-2"},{"key":"bibr15-09645284241248465","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.613006"},{"key":"bibr16-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020006075"},{"key":"bibr17-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-019-0267-y"},{"key":"bibr18-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1111\/1756-185X.12939"},{"key":"bibr19-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.05.045"},{"issue":"5","key":"bibr20-09645284241248465","first-page":"1719","volume":"34","author":"Liu EH","year":"2020","journal-title":"J Biol Regul Homeost Agents"},{"key":"bibr21-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8071234"},{"key":"bibr22-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1097\/01.brs.0000222019.84095.23"},{"key":"bibr23-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-017-1358-2"},{"key":"bibr24-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-012-0708-3"},{"key":"bibr25-09645284241248465","doi-asserted-by":"publisher","DOI":"10.2147\/JPR.S199798"},{"key":"bibr26-09645284241248465","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v26.i19.2440"},{"key":"bibr27-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000002071"},{"key":"bibr28-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1016\/j.explore.2021.10.012"},{"key":"bibr29-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000028540"},{"key":"bibr30-09645284241248465","first-page":"1092","volume":"2017","author":"Zhou S","journal-title":"Shanghai J Acupunct Moxibustion"},{"key":"bibr31-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-019-3038-2"},{"issue":"11","key":"bibr32-09645284241248465","first-page":"835","volume":"44","author":"Maiji MW","year":"2019","journal-title":"Zhen Ci Yan Jiu"},{"key":"bibr33-09645284241248465","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.12131"},{"key":"bibr34-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116962"},{"key":"bibr35-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.07.088"},{"key":"bibr36-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.12.045"},{"key":"bibr37-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0000000000001982"},{"key":"bibr38-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-017-0508-6"},{"key":"bibr39-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.24573"},{"key":"bibr40-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-9-111"},{"key":"bibr41-09645284241248465","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1402565"},{"key":"bibr42-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-4401"},{"key":"bibr43-09645284241248465","doi-asserted-by":"publisher","DOI":"10.1007\/s13577-020-00373-3"}],"container-title":["Acupuncture in Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09645284241248465","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09645284241248465","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09645284241248465","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T09:19:27Z","timestamp":1777367967000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09645284241248465"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,3]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1177\/09645284241248465"],"URL":"https:\/\/doi.org\/10.1177\/09645284241248465","relation":{},"ISSN":["0964-5284","1759-9873"],"issn-type":[{"value":"0964-5284","type":"print"},{"value":"1759-9873","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,3]]}}}