{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:56:44Z","timestamp":1777381004285,"version":"3.51.4"},"reference-count":36,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,5,11]],"date-time":"2025-05-11T00:00:00Z","timestamp":1746921600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100017577","name":"Basic Public Welfare Research Program of Zhejiang Province","doi-asserted-by":"publisher","award":["LY20H270015"],"award-info":[{"award-number":["LY20H270015"]}],"id":[{"id":"10.13039\/501100017577","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007194","name":"Wenzhou Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["Y20190198"],"award-info":[{"award-number":["Y20190198"]}],"id":[{"id":"10.13039\/501100007194","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81674053"],"award-info":[{"award-number":["81674053"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Acupunct Med"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>The aim of this study was to investigate the effect of electroacupuncture (EA) on macrophage polarization and bone erosion in a mouse model of collagen-induced arthritis (CIA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>C57BL\/B6 mice were used to establish a CIA model and were treated with electroacupuncture (EA) at ST36 and SP6. At the end of the experiment, knee joints were harvested for hematoxylin-eosin (H&amp;E) staining to detect knee synovitis. Immunohistochemistry (IHC) was performed to assess the expression of macrophage markers. The degree of bone destruction was evaluated using micro-computed tomography (CT), tartrate-resistant acid phosphatase (TRAP) staining and safranin-O fast green staining. Peripheral blood transcriptome sequencing was performed using Illumina high-throughput sequencing. Synovial membrane proteins were quantitatively analyzed by mass spectrometry. Differentially expressed genes and proteins were identified and the R software package was used to analyze the data.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Compared with the model group, the arthritis index (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) and inflammatory infiltration decreased (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05), cartilage destruction was inhibited (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.01), the number of osteoclasts decreased (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05), knee bone erosion was alleviated and the M1\/M2 macrophage ratio decreased (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.01) in the EA group. The results of bioinformatics analysis showed that the differential genes between the EA and model groups were mainly enriched in rheumatoid arthritis (RA) and the peroxisome proliferator-activated receptor (PPAR) signaling pathway. Differentially expressed proteins were mostly enriched in the toll-like receptor (TLR) signaling and autophagy pathways.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>EA prevents bone erosion, reduces the M1\/M2 macrophage ratio in synovial tissue, inhibits the TLR and autophagy pathways and reduces synovial invasion in a mouse model of CIA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09645284251331991","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T02:12:24Z","timestamp":1747015944000},"page":"150-163","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Electroacupuncture attenuates bone erosion and promotes macrophage polarization in a mouse model of collagen-induced arthritis"],"prefix":"10.1177","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4310-952X","authenticated-orcid":false,"given":"Shi-Ying","family":"Dong","sequence":"first","affiliation":[{"name":"Wenzhou Medical University, Wenzhou, China"},{"name":"The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhou","sequence":"additional","affiliation":[{"name":"Wenzhou Medical University, Wenzhou, China"},{"name":"The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Chi","family":"Wang","sequence":"additional","affiliation":[{"name":"Wenzhou Medical University, Wenzhou, China"},{"name":"The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong-Heng","family":"Du","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian-Shen","family":"Ye","sequence":"additional","affiliation":[{"name":"Wenzhou Medical University, Wenzhou, China"},{"name":"The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,5,11]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.125278"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.2174\/1874312901105010100"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.33418"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10545"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2018.10.016"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01958"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0939-8"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2017.09.002"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-020-02251-w"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/1614627"},{"issue":"4","key":"e_1_3_3_12_2","first-page":"498","article-title":"Macrophage activity assessed by soluble CD163 in early rheumatoid arthritis: association with disease activity but different response patterns to synthetic and biologic DMARDs","volume":"33","author":"Greisen SR","year":"2015","unstructured":"Greisen SR, M\u00f8ller HJ, Stengaard-Pedersen K, et al. Macrophage activity assessed by soluble CD163 in early rheumatoid arthritis: association with disease activity but different response patterns to synthetic and biologic DMARDs. Clin Exp Rheumatol 2015; 33(4): 498\u2013502.","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2017-212880"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.5RU0519-145R"},{"key":"e_1_3_3_15_2","unstructured":"Effect of electro-acupuncture (EA) and manual acupuncture (MA) on markers of inflammation in knee osteoarthritis. PubMed. https:\/\/pubmed.ncbi.nlm.nih.gov\/32904642\/ (accessed 8 May 2023)"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.173"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2006.02508.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2009.08.009"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/09645284221076515"},{"issue":"5","key":"e_1_3_3_20_2","first-page":"740","article-title":"Mechanism of electroacupuncture on inflammatory pain: neural-immune-endocrine interactions","volume":"39","author":"Li Y","year":"2019","unstructured":"Li Y, Yang M, Wu F, et al. Mechanism of electroacupuncture on inflammatory pain: neural-immune-endocrine interactions. J Tradit Chin Med 2019; 39(5): 740\u2013749.","journal-title":"J Tradit Chin Med"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.813748"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1566-0702(02)00035-8"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03402177"},{"key":"e_1_3_3_24_2","first-page":"6436420","article-title":"Moxibustion of Zusanli (ST36) and Shenshu (BL23) alleviates cartilage degradation through RANKL\/OPG signaling in a rabbit model of rheumatoid arthritis","volume":"2019","author":"Chen Y","year":"2019","unstructured":"Chen Y, Li H, Luo X, et al. Moxibustion of Zusanli (ST36) and Shenshu (BL23) alleviates cartilage degradation through RANKL\/OPG signaling in a rabbit model of rheumatoid arthritis. Evid Based Complement Alternat Med 2019; 2019: 6436420.","journal-title":"Evid Based Complement Alternat Med"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/5741931"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1164157"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8295580"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-00541-3"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26429"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00003"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.04.028"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-015-8473-z"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/9050763"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/MJT.0000000000000477"},{"key":"e_1_3_3_35_2","first-page":"552","article-title":"[Berberine inhibits autophagy and promotes apoptosis of fibroblast-like synovial cells from rheumatoid arthritis patients through the ROS\/mTOR signaling pathway]","volume":"43","author":"Zong S","year":"2023","unstructured":"Zong S, Zhou J, Cai W, et al. [Berberine inhibits autophagy and promotes apoptosis of fibroblast-like synovial cells from rheumatoid arthritis patients through the ROS\/mTOR signaling pathway]. Nan Fang Yi Ke Da Xue Xue Bao 2023; 43: 552\u2013559.","journal-title":"Nan Fang Yi Ke Da Xue Xue Bao"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2018.11.003"},{"issue":"5","key":"e_1_3_3_37_2","first-page":"2065","article-title":"Autophagy inhibitor regulates apoptosis and proliferation of synovial fibroblasts through the inhibition of PI3K\/AKT pathway in collagen-induced arthritis rat model","volume":"9","author":"Li S","year":"2017","unstructured":"Li S, Chen JW, Xie X, et al. Autophagy inhibitor regulates apoptosis and proliferation of synovial fibroblasts through the inhibition of PI3K\/AKT pathway in collagen-induced arthritis rat model. Am J Transl Res 2017; 9(5): 2065\u20132076.","journal-title":"Am J Transl Res"}],"container-title":["Acupuncture in Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09645284251331991","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09645284251331991","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09645284251331991","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T09:19:42Z","timestamp":1777367982000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09645284251331991"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,11]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1177\/09645284251331991"],"URL":"https:\/\/doi.org\/10.1177\/09645284251331991","relation":{},"ISSN":["0964-5284","1759-9873"],"issn-type":[{"value":"0964-5284","type":"print"},{"value":"1759-9873","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,11]]}}}