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The aim of this study was to investigate how electroacupuncture (EA) affects inflammation-related protein levels, including NLRP3, caspase-1 and ASC, in a mouse model of IBD and explore the underlying mechanisms of action.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Forty-eight mice were randomly assigned to control, model, EA and NLRP3 inhibitor groups. IBD was induced using dextran sodium sulfate (DSS). After 1\u2009week of EA treatment, fecal occult blood tests were performed and disease activity index (DAI) scores were recorded. In addition, hematoxylin-eosin staining was performed to evaluate the histopathological changes in the colon. Serum interleukin (IL)-1\u03b2, IL-18 and tumor necrosis factor (TNF)-\u03b1 levels were measured using enzyme-linked immunosorbent assay, and protein expression of NLRP3, caspase-1, ASC, gasdermin D (GSDMD), N-terminal GSDMD (N-GSDMD), IL-1\u03b2 and IL-18 in colonic tissues was assessed using Western blotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      IBD resulted in alterations in general condition and colonic morphology, a significant increase in serum IL-1\u03b2, IL-18 and TNF-\u03b1 levels, and a significant increase in protein expression of NLRP3, caspase-1, ASC, GSDMD, N-GSDMD, IL-1\u03b2 and IL-18 in the colonic tissues (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.01 or\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001). EA and NLRP3 inhibition reversed these pathological changes and reduced the expression of inflammatory proteins induced by intestinal inflammatory responses (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.05 or\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The NLRP3\/caspase-1\/ASC signaling pathway appears to play a role in mediating the therapeutic effects of EA in IBD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09645284261423205","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T10:01:29Z","timestamp":1774000889000},"page":"157-166","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Electroacupuncture inhibits the NLRP3\/caspase-1\/ASC signaling pathway in a mouse model of inflammatory bowel disease"],"prefix":"10.1177","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4446-6437","authenticated-orcid":false,"given":"Lv","family":"Jia","sequence":"first","affiliation":[{"name":"School of Acupuncture-Moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3277-1333","authenticated-orcid":false,"given":"Sihui","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Acupuncture-Moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Acupuncture-Moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8211-7568","authenticated-orcid":false,"given":"Zuqiang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Acupuncture-Moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penghui","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Acupuncture-Moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5634-1555","authenticated-orcid":false,"given":"Qiong","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Acupuncture-Moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Acupuncture-Moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,3,20]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0268-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1426803"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-019-0245-x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2024.176331"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.116679"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1232629"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-025-01218-7"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/cns.70572"},{"key":"e_1_3_3_10_2","first-page":"1398","article-title":"Electroacupuncture in animal models of inflammatory bowel disease: a systematic review and meta-analysis","volume":"18","author":"Xu X","year":"2023","unstructured":"Xu X, Chen L, Ding PT, et al Electroacupuncture in animal models of inflammatory bowel disease: a systematic review and meta-analysis. 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