{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:34:20Z","timestamp":1792787660730,"version":"4.5.0"},"reference-count":29,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Research on the Mechanism of Acupuncture of Heart Meridian to Prevent Heart Failure Based on the Theory of Prevention of Disease","award":["2024LC090"],"award-info":[{"award-number":["2024LC090"]}]},{"DOI":"10.13039\/501100012166","name":"national key research and development program of china","doi-asserted-by":"publisher","award":["2022YFC3500500"],"award-info":[{"award-number":["2022YFC3500500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"national key research and development program of china","doi-asserted-by":"publisher","award":["2022YFC3500502"],"award-info":[{"award-number":["2022YFC3500502"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Acupunct Med"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To investigate the role and mechanism of the dorsal motor nucleus of the vagus nerve (DMV) in the effects of electroacupuncture (EA) at HT7 in a mouse model of chronic heart failure (CHF).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A CHF model was established in male C57 mice by ligating the anterior descended branch of the left coronary artery. In part I of the experiment, mice were randomly divided into Control, untreated model (CHF) and EA-treated model (CHF\u2009+\u2009EA) groups (n\u2009=\u20096 each). In part II, mice were randomly divided into the following groups (n\u2009=\u20096 each): EA-treated model group (CHF\u2009+\u2009EA); EA-treated model group receiving cholinergic neuron promoter chemogenetic activating virus encoding human M4 muscarinic (hM4) receptor (hM4D) and clozapine-N-oxide (CNO) (CHF\u2009+\u2009hM4D\u2009+\u2009CNO\u2009+\u2009EA); EA-treated model group additionally receiving \u201cempty\u201d virus encoding enhanced green fluorescence protein (EGFP) and CNO (CHF\u2009+\u2009EFGP\u2009+\u2009CNO\u2009+\u2009EA); EA-treated model group receiving hM4D only (CHF\u2009+\u2009hM4D\u2009+\u2009EA); and EA-treated model group receiving CNO only (CHF\u2009+\u2009CNO\u2009+\u2009EA). All mice except those in the Control and CHF groups were treated with bilateral EA at HT7 (frequency 2\u2009Hz, intensity 2mA, pulse width 0.2\u2009ms\u2009\u00b1\u200920%) once a day (20\u2009min each time) for 7\u2009days. Left ventricular ejection fraction (LVEF), left ventricular fractional shortening (LVFS), scar area and serum inflammatory factor levels were evaluated using a Doppler ultrasound imaging system, Masson staining, ELISA and other methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Compared with the Control group, LVEF\/LVFS in the CHF group decreased (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001), the scar area was larger, atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and interleukin (IL)-6 levels increased significantly, and acetylcholine (ACh) decreased significantly (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001) in the CHF group. Compared with the CHF group, LVEF\/LVFS increased (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001), scar area was smaller, ANP, BNP and IL-6 decreased significantly and ACh increased significantly (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001) in the CHF\u2009+\u2009EA group. Compared with the CHF\u2009+\u2009EA group, the CHF\u2009+\u2009hM4D\u2009+\u2009CNO\u2009+\u2009EA group exhibited a decrease in LVEF\/LVFS (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001), a larger scar area, a significant increase in ANP, BNP and IL-6, and a significant decrease in ACh (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001),while there was no significant difference in each index between the CHF\u2009+\u2009CNO\u2009+\u2009EA and CHF\u2009+\u2009hM4D\u2009+\u2009EA groups (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&gt;\u20090.05). Compared with the CHF\u2009+\u2009hM4D\u2009+\u2009CNO\u2009+\u2009EA group, the CHF\u2009+\u2009EGFP\u2009+\u2009CNO\u2009+\u2009EA exhibited an increase in LVEF\/LVFS (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001), a smaller scar area, a significant decrease in ANP, BNP and IL-6, and a significant increase in ACh (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      Electroacupuncture at (HT7) may activate DMV\n                      <jats:sup>Ach<\/jats:sup>\n                      and reduce myocardial fibrosis, thereby improving CHF, in a mouse model.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09645284261424260","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T19:18:47Z","timestamp":1773256727000},"page":"167-176","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Cholinergic neurons in the dorsal nucleus of the vagus nerve are involved in the mechanism of action of electroacupuncture at HT7 in a mouse model of chronic heart failure"],"prefix":"10.1177","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3468-656X","authenticated-orcid":false,"given":"Shi-yue","family":"Wang","sequence":"first","affiliation":[{"name":"College of Acupuncture and Massage, Anhui University of Chinese Medicine, Hefei, China"},{"name":"College of Moxibustion and Tuina, Anhui University of Chinese Medicine, Hefei, China"},{"name":"Anhui Province Key Laboratory of Meridian Viscera Correlationship, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9134-2764","authenticated-orcid":false,"given":"Hao-sheng","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Acupuncture and Massage, Anhui University of Chinese Medicine, Hefei, China"},{"name":"Anhui Province Key Laboratory of Meridian Viscera Correlationship, Hefei, China"},{"name":"College of Traditional Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-bing","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Acupuncture and Massage, Anhui University of Chinese Medicine, Hefei, China"},{"name":"College of Moxibustion and Tuina, Anhui University of Chinese Medicine, Hefei, China"},{"name":"Anhui Province Key Laboratory of Meridian Viscera Correlationship, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Meiqi Zhou","sequence":"additional","affiliation":[{"name":"College of Acupuncture and Massage, Anhui University of Chinese Medicine, Hefei, China"},{"name":"College of Moxibustion and Tuina, Anhui University of Chinese Medicine, Hefei, China"},{"name":"Anhui Province Key Laboratory of Meridian Viscera Correlationship, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,3,11]]},"reference":[{"key":"e_1_3_2_2_2","unstructured":"Lu L Li L Li M et al. Analysis of the Application Status of Evaluation Indicators in the Treatment of Chronic Heart Failure with Traditional Chinese Medicine [J]. Tianjin J Tradit Chin Med 2022; 39(1): 123\u2013130."},{"issue":"11","key":"e_1_3_2_3_2","first-page":"1649","article-title":"On the theoretical basis and application of meridian connection method in the treatment of chronic heart failure","volume":"19","author":"Zhao J","year":"2024","unstructured":"Zhao J, Yan S, Wang L, et al. On the theoretical basis and application of meridian connection method in the treatment of chronic heart failure. World J Tradit Chin Med 2024; 19(11): 1649\u20131652.","journal-title":"World J Tradit Chin Med"},{"key":"e_1_3_2_4_2","volume-title":"World Heart Report 2023: Confronting the World\u2019s Number One Killer","author":"World Heart Federation","year":"2023","unstructured":"World Heart Federation. World Heart Report 2023: Confronting the World\u2019s Number One Killer. Geneva: World Heart Federation, 2023."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10741-024-10430-w"},{"issue":"21","key":"e_1_3_2_6_2","first-page":"5","article-title":"Effects of electroacupuncture internal closing at different frequencies on \u03b23-ADR expression in the hypothalamus of rats with myocardial ischemia\u2013reperfusion injury","volume":"19","author":"Lin Y","year":"2009","unstructured":"Lin Y, Wang X, Yi S, et al. Effects of electroacupuncture internal closing at different frequencies on \u03b23-ADR expression in the hypothalamus of rats with myocardial ischemia\u2013reperfusion injury. Chin J Mod Med 2009; 19(21): 5.","journal-title":"Chin J Mod Med"},{"issue":"3","key":"e_1_3_2_7_2","first-page":"225","article-title":"Research ideas on the possible synergistic and detoxifying effects of acupuncture on digitalis drugs for heart failure","volume":"36","author":"Ma S","year":"2011","unstructured":"Ma S, Gao J, Wang Y, et al. Research ideas on the possible synergistic and detoxifying effects of acupuncture on digitalis drugs for heart failure. Acupunct Res 2011; 36(3): 225\u2013229.","journal-title":"Acupunct Res"},{"issue":"2","key":"e_1_3_2_8_2","first-page":"213","article-title":"Effect and mechanism of electroacupuncture at Zhongwan point on gastric injury response induced by acid stimulation in rats","volume":"65","author":"Shi H","year":"2024","unstructured":"Shi H, Yang Z, Wang X, et al. Effect and mechanism of electroacupuncture at Zhongwan point on gastric injury response induced by acid stimulation in rats. Chin J Tradit Chin Med 2024; 65(2): 213\u2013218.","journal-title":"Chin J Tradit Chin Med"},{"key":"e_1_3_2_9_2","volume-title":"Gastric sensory-motor neuron\u2013mediated acupuncture effect in the dorsal vagus complex","author":"Li S","year":"2023","unstructured":"Li S. Gastric sensory-motor neuron\u2013mediated acupuncture effect in the dorsal vagus complex. Dissertation. China Academy of Chinese Medical Sciences, Beijing, China, 2023."},{"issue":"1","key":"e_1_3_2_10_2","first-page":"4","article-title":"Research status of the influence of acupuncture on vagus nerve function","volume":"26","author":"Zeng Y","year":"2011","unstructured":"Zeng Y, Wang M, Mei Z. Research status of the influence of acupuncture on vagus nerve function. Chin J Tradit Chin Med 2011; 26(1): 4.","journal-title":"Chin J Tradit Chin Med"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.223925"},{"key":"e_1_3_2_12_2","volume-title":"Study on myocardial cell apoptosis and changes in pulmonary vascular structure in rats with heart failure and drug intervention","author":"Wu Z","year":"2004","unstructured":"Wu Z. Study on myocardial cell apoptosis and changes in pulmonary vascular structure in rats with heart failure and drug intervention. Dissertation. Peking Union Medical College, Beijing, China, 2004."},{"key":"e_1_3_2_13_2","unstructured":"Names locations of common acupoints in experimental animals-Part 3: mice. Acupuncture Research 2021; 46(05): 445\u2013446."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/heartjnl-2022-322030"},{"issue":"6785","key":"e_1_3_2_15_2","first-page":"458","article-title":"The inflammatory reflex","volume":"405","author":"Tracey KJ","year":"2000","unstructured":"Tracey KJ, Borovikova LV. The inflammatory reflex. Nature 2000; 405(6785): 458\u2013462.","journal-title":"Nature"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hrthm.2015.06.005"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10557-020-07062-1"},{"key":"e_1_3_2_18_2","first-page":"1","article-title":"Traditional Chinese medicine regulation of NF-\u03baB signaling pathway intervention in the progression of chronic heart failure","volume":"2024","author":"Lian K","unstructured":"Lian K, Deng Y, Tang S, et al. Traditional Chinese medicine regulation of NF-\u03baB signaling pathway intervention in the progression of chronic heart failure. Chin J Exp Pharmacol 2024: 1\u201313.","journal-title":"Chin J Exp Pharmacol"},{"issue":"1","key":"e_1_3_2_19_2","first-page":"163","article-title":"Electroacupuncture combined with massage in the treatment of knee osteoarthritis and its effect on the expression levels of IL-1\u03b2, IL-6, TNF-\u03b1, MMP-3 and TIMP-1","volume":"50","author":"Ye B","year":"2023","unstructured":"Ye B, Ye L, Liao W, et al. Electroacupuncture combined with massage in the treatment of knee osteoarthritis and its effect on the expression levels of IL-1\u03b2, IL-6, TNF-\u03b1, MMP-3 and TIMP-1. J Liaoning Tradit Chin Med 2023; 50(1): 163\u2013166.","journal-title":"J Liaoning Tradit Chin Med"},{"issue":"3","key":"e_1_3_2_20_2","first-page":"752","article-title":"Comparative study on the effects of real and sham acupuncture at Shenmen point on heart rate and heart rate variability","volume":"23","author":"Lin R","year":"2012","unstructured":"Lin R, Wu J, Zhang P, et al. Comparative study on the effects of real and sham acupuncture at Shenmen point on heart rate and heart rate variability. Shi Zhen Tradit Chin Med 2012; 23(3): 752\u2013754.","journal-title":"Shi Zhen Tradit Chin Med"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a028456"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20110340"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2014.12.002"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-012-1200-1"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1139\/y01-039"},{"issue":"2","key":"e_1_3_2_26_2","first-page":"1","article-title":"Electroacupuncture pretreatment as a novel avenue to protect the heart against ischemia and reperfusion injury","volume":"2020","author":"Zhang J","year":"2020","unstructured":"Zhang J, Zhu L, Li H, et al. Electroacupuncture pretreatment as a novel avenue to protect the heart against ischemia and reperfusion injury. Evid Based Complement Alternat Med 2020; 2020(2): 1\u20139.","journal-title":"Evid Based Complement Alternat Med"},{"issue":"24","key":"e_1_3_2_27_2","first-page":"6072","article-title":"Effects of beta-blockers carvedilol and metoprolol on body fluid factors and ANP, TNF-\u03b1, IL-6, and NE in rats with heart failure","volume":"38","author":"Zhang N","year":"2018","unstructured":"Zhang N, Li T, Li K, et al. Effects of beta-blockers carvedilol and metoprolol on body fluid factors and ANP, TNF-\u03b1, IL-6, and NE in rats with heart failure. Chin J Gerontol 2018; 38(24): 6072\u20136074.","journal-title":"Chin J Gerontol"},{"issue":"4","key":"e_1_3_2_28_2","first-page":"406","article-title":"Vagus nerve stimulation in the application of chronic heart failure","volume":"4","author":"Chen R","year":"2016","unstructured":"Chen R, Chen K. Vagus nerve stimulation in the application of chronic heart failure. J Nanjing Med Univ Nat Sci Ed 2016; 4(4): 406\u2013410.","journal-title":"J Nanjing Med Univ Nat Sci Ed"},{"issue":"6","key":"e_1_3_2_29_2","first-page":"547","article-title":"Research progress on changes of vagus nerve activity in heart failure","volume":"26","author":"Ji M","year":"2012","unstructured":"Ji M, Zhou Q, Hou Y. Research progress on changes of vagus nerve activity in heart failure. Chin J Card Pacemaker Electrophysiol 2012; 26(6): 547\u2013549.","journal-title":"Chin J Card Pacemaker Electrophysiol"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjog.2012.04.003"}],"container-title":["Acupuncture in Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09645284261424260","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09645284261424260","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09645284261424260","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:56:43Z","timestamp":1792666603000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09645284261424260"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,11]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1177\/09645284261424260"],"URL":"https:\/\/doi.org\/10.1177\/09645284261424260","relation":{},"ISSN":["0964-5284","1759-9873"],"issn-type":[{"value":"0964-5284","type":"print"},{"value":"1759-9873","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,11]]}}}