{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:35:41Z","timestamp":1792787741154,"version":"4.5.0"},"reference-count":26,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82205255"],"award-info":[{"award-number":["82205255"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2022JJ40301"],"award-info":[{"award-number":["2022JJ40301"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2022JJ40312"],"award-info":[{"award-number":["2022JJ40312"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Undergraduate Research Innovation Fund of Hunan University of Traditional Chinese Medicine","award":["2023BKS093"],"award-info":[{"award-number":["2023BKS093"]}],"id":[{"id":"https:\/\/ror.org\/05qfq0x09","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Acupunct Med"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Neurogenic bladder (NB) is a neurological bladder dysfunction characterized by increased contraction of the detrusor muscle. Adenosine triphosphate (ATP)-P2X receptor signaling influences muscle contraction. The aim of this study was to determine whether ATP-P2X signaling affects the mechanism by which electroacupuncture (EA) influences excessive detrusor muscle contraction in a rat model of NB.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Forty-eight rats were divided into CON (n\u2009=\u20098), SHAM (n\u2009=\u20098) and MODEL (n\u2009=\u200932) groups. After the latter group underwent model establishment, 24 successfully modeled rats were randomly divided into the NB, pyridoxalphosphate-6-azophenyl-2\u2032,4\u2032-disulfonic acid (PPADS; P2X\n                      <jats:sub>1\/2<\/jats:sub>\n                      receptor antagonist) and EA groups (n\u2009=\u20098 each). The NB model was established using a modified Hassan Shaker spinal cord transection method. One week after EA treatment, urodynamic tests were used to assess bladder function, morphological changes were evaluated in hematoxylin and eosin stained bladder sections, enzyme-linked immunosorbent assays were used to measure ATP concentrations, and Western blotting was used to analyze the protein levels of P2X\n                      <jats:sub>1<\/jats:sub>\n                      , P2X\n                      <jats:sub>2<\/jats:sub>\n                      , phosphorylated myosin light chain kinase (p-MLCK) and phosphorylated myosin light chain (p-MLC).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      NB treatment led to morphological abnormalities, impaired urodynamics, increased ATP\/P2X\n                      <jats:sub>1<\/jats:sub>\n                      \/P2X\n                      <jats:sub>2<\/jats:sub>\n                      \/p-MLC levels (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.01) and decreased p-MLCK protein levels (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.01). EA treatment significantly improved the morphological abnormalities and impaired urodynamics. Compared with NB, both EA and PPADS significantly reduced ATP\/P2X1\/P2X2\/p-MLC levels (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.01) and increased the expression of p-MLCK (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The ATP-P2X signaling pathway may be involved in the mitigating effect of EA on excessive detrusor contractions in this rat model of NB.<\/jats:p>\n                  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