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Segments of stenotic saphenous vein grafts were explanted from 14 patients at re-do bypass operation and ten normal saphenous veins were harvested during femoro-popliteal grafting. Sections of specimens were analysed using cell type specific antibodies to identify dendritic cells (CD1a, S-100), T-lymphocytes (CD3), macrophages (CD68), smooth muscle cells (\u03b1-SMA) and endothelial cells (FVI11). Dual immunostaining, confocal immunofluorescent laser scanning microscopy and electron microscopy were used. Stenotic grafts showed structural alterations of intimal hyperplasia and varying degrees of atherosclerotic degeneration. No cells expressing CD1a and S-100 were observed in the intima and media of normal saphenous veins. Cells expressing these antigens were present around areas of medial neovascularization and within intimal atherosclerotic lesions in saphenous vein bypass grafts. Electron microscopy demonstrated the presence of cells containing a well-developed tubulovesicular system which is unique to cells from the dendritic cell family. Double immunohistochemistry and confocal immunofluorescent microscopy revealed the co-localization of T-lymphocytes with dendritic cells. Dendritic cells are present in stenotic saphenous vein bypass grafts. Dendritic cells may be responsible for antigen presentation and modulation of immune reactions in accelerated graft atherosclerosis through their interaction with T-lymphocytes.<\/jats:p>","DOI":"10.1177\/096721090100900220","type":"journal-article","created":{"date-parts":[[2024,9,22]],"date-time":"2024-09-22T05:13:09Z","timestamp":1726981989000},"page":"194-200","source":"Crossref","is-referenced-by-count":4,"title":["Immunohistochemical and Ultrastructural Evidence that Dendritic Cells Infiltrate Stenotic Aortocoronary Saphenous Vein Bypass Grafts"],"prefix":"10.1177","volume":"9","author":[{"given":"S.M.","family":"Cherian","sequence":"first","affiliation":[{"name":"Surgical Professorial Unit, St Vincent's Hospital, University of New South Wales, Victoria Street, Level 17 O'Brien Building, Darlinghurst, NSW 2010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.V.","family":"Bobryshev","sequence":"additional","affiliation":[{"name":"Surgical Professorial Unit, St Vincent's Hospital, University of New South Wales, Victoria Street, Level 17 O'Brien Building, Darlinghurst, NSW 2010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Liang","sequence":"additional","affiliation":[{"name":"Surgical Professorial Unit, St Vincent's Hospital, University of New South Wales, Victoria Street, Level 17 O'Brien Building, Darlinghurst, NSW 2010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.J.","family":"Inder","sequence":"additional","affiliation":[{"name":"Surgical Professorial Unit, St Vincent's Hospital, University of New South Wales, Victoria Street, Level 17 O'Brien Building, Darlinghurst, NSW 2010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.Y.","family":"Wang","sequence":"additional","affiliation":[{"name":"Surgical Professorial Unit, St Vincent's Hospital, University of New South Wales, Victoria Street, Level 17 O'Brien Building, Darlinghurst, NSW 2010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.S.A.","family":"Lord","sequence":"additional","affiliation":[{"name":"Surgical Professorial Unit, St Vincent's Hospital, University of New South Wales, Victoria Street, Level 17 O'Brien Building, Darlinghurst, NSW 2010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Tran","sequence":"additional","affiliation":[{"name":"Surgical Professorial Unit, St Vincent's Hospital, University of New South Wales, Victoria Street, Level 17 O'Brien Building, Darlinghurst, NSW 2010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Pandey","sequence":"additional","affiliation":[{"name":"School of Anatomy, University of New South Wales, Sydney, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Halasz","sequence":"additional","affiliation":[{"name":"School of Anatomy, University of New South Wales, Sydney, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.E.","family":"Farnsworth","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, St Vincent's Hospital, Sydney, NSW 2010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2001,4]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Ischemic Heart Disease. 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