{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:07:50Z","timestamp":1777928870684,"version":"3.51.4"},"reference-count":6,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2016,6,22]],"date-time":"2016-06-22T00:00:00Z","timestamp":1466553600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Med Screen"],"published-print":{"date-parts":[[2016,12]]},"abstract":"\n Objective<\/jats:title>\n To determine (i) if electrospray mass spectrometry\u2013mass spectrometry with the SpOtOn Diagnostics Ltd reagent kit for sickle cell screening could be integrated into the English newborn screening programme, under routine screening conditions, and provide mass spectrometry\u2013mass spectrometry results which match existing methods, and (ii) if common action values could be set for all manufacturers in the study, for all assessed haemoglobins, to indicate which samples require further investigation.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Anonymised residual blood spots were analysed using the SpOtOn reagent kit as per manufacturer\u2019s instructions, in parallel with existing techniques at four laboratories. Mass spectrometry\u2013mass spectrometry instrumentation at Laboratories A and B was AB Sciex (Warrington, UK) AP4000, and at Laboratories C and D, Waters Micromass (Manchester, UK), Xevo TQMS and Premier, respectively.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n There were 23,898 results accepted from the four laboratories. Excellent specificity at 100% sensitivity was observed for haemoglobin S, haemoglobin C, haemoglobin E and haemoglobin O\n Arab<\/jats:sup>\n . A common action value was not possible for Hb C, but action values were set by manufacturer. The two haemoglobin D\n Punjab<\/jats:sup>\n cases at Laboratory D were not detected using the common action value. Conversely, false-positive results with haemoglobin D\n Punjab<\/jats:sup>\n were a problem at the remaining three laboratories.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n This multicentre study demonstrates that it is possible to implement mass spectrometry\u2013mass spectrometry into an established screening programme while maintaining consistency with existing methods for haemoglobinopathy screening. However, one of the instruments investigated cannot be recommended for use with this application.<\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/0969141316631008","type":"journal-article","created":{"date-parts":[[2016,3,10]],"date-time":"2016-03-10T20:08:39Z","timestamp":1457640519000},"page":"175-178","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Newborn screening for sickling and other haemoglobin disorders using tandem mass spectrometry: A pilot study of methodology in laboratories in England"],"prefix":"10.1177","volume":"23","author":[{"given":"Yvonne A","family":"Daniel","sequence":"first","affiliation":[{"name":"Public Health England, PHE Sickle Cell and Thalassaemia Screening Programme, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan","family":"Henthorn","sequence":"additional","affiliation":[{"name":"Public Health England, PHE Sickle Cell and Thalassaemia Screening Programme, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2016,6,22]]},"reference":[{"key":"bibr1-0969141316631008","unstructured":"Daniel Y and Henthorn J.\n Handbook for laboratories\n . 3rd ed. London: NHS Sickle Cell & Thalassaemia Screening Programme, 2012."},{"key":"bibr2-0969141316631008","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05646.x"},{"key":"bibr3-0969141316631008","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V112.11.2387.2387"},{"key":"bibr4-0969141316631008","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.106369"},{"key":"bibr5-0969141316631008","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2011.04.031"},{"key":"bibr6-0969141316631008","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2013.210948"}],"container-title":["Journal of Medical Screening"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0969141316631008","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0969141316631008","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0969141316631008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T23:23:49Z","timestamp":1777677829000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0969141316631008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,22]]},"references-count":6,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1177\/0969141316631008"],"URL":"https:\/\/doi.org\/10.1177\/0969141316631008","relation":{},"ISSN":["0969-1413","1475-5793"],"issn-type":[{"value":"0969-1413","type":"print"},{"value":"1475-5793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,22]]}}}