{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:08:37Z","timestamp":1777928917903,"version":"3.51.4"},"reference-count":11,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2016,10,21]],"date-time":"2016-10-21T00:00:00Z","timestamp":1477008000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Med Screen"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To determine whether maternal plasma collected in cell-free DNA stabilizing tubes is suitable for measuring prenatal screening \u2018serum\u2019 markers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Matched plasma and serum samples were collected from 41\u2009second trimester and 42 first trimester non-Down\u2019s syndrome pregnancies. Second trimester samples were tested for alpha-fetoprotein, unconjugated estriol, human chorionic gonadotropin, and inhibin-A (Beckman Coulter DxI immunoassay). First trimester samples were tested for human chorionic gonadotropin and pregnancy-associated plasma protein A. Method comparisons performed for each marker compared plasma and serum results. Down\u2019s syndrome likelihood ratios in serum and plasma were compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Plasma and serum results for all markers were highly correlated ( r\u2009&gt;\u20090.983) but for all, plasma results differed, usually by proportional amounts. After conversion to multiples of the median using sample type-specific medians, the logarithmic standard deviations in serum and plasma did not differ (all p\u2009&gt;\u20090.37). Likelihood ratios for the first and second trimester marker combinations were highly correlated and closely agreed (log likelihood ratios range 1.005 to 1.032; 1.000 indicates complete agreement).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results using specialized plasma collection tubes are similar to those of our earlier study showing that plasma collected in EDTA tubes is suitable for \u2018serum\u2019 Down\u2019s syndrome screening. Laboratories must account for proportional changes by computing new plasma medians or modifying existing serum medians. Using a portion of the plasma from cell-free DNA collection tubes for \u2018serum screening\u2019 may have an advantage in programmes that are reflexively testing cell-free DNA, as only one sample type need be collected.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0969141316670193","type":"journal-article","created":{"date-parts":[[2016,10,21]],"date-time":"2016-10-21T20:08:10Z","timestamp":1477080490000},"page":"113-119","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Measuring maternal serum screening markers for Down\u2019s syndrome in plasma collected for cell-free DNA testing"],"prefix":"10.1177","volume":"24","author":[{"given":"Geralyn M","family":"Lambert-Messerlian","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Women & Infants Hospital, Providence, RI, USA"},{"name":"Department of Pathology and Laboratory Medicine, Alpert Medical School, Brown University, Providence, RI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth E","family":"Eklund","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Women & Infants Hospital, Providence, RI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis M","family":"Neveux","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Women & Infants Hospital, Providence, RI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenn E","family":"Palomaki","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Women & Infants Hospital, Providence, RI, USA"},{"name":"Department of Pathology and Laboratory Medicine, Alpert Medical School, Brown University, Providence, RI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2016,10,21]]},"reference":[{"key":"bibr1-0969141316670193","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0319-CP"},{"key":"bibr2-0969141316670193","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)02174-0"},{"key":"bibr3-0969141316670193","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808319105"},{"key":"bibr4-0969141316670193","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810641105"},{"key":"bibr5-0969141316670193","doi-asserted-by":"publisher","DOI":"10.1002\/uog.14791"},{"key":"bibr6-0969141316670193","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2015.03.043"},{"key":"bibr7-0969141316670193","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2393-14-229"},{"key":"bibr8-0969141316670193","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4658"},{"key":"bibr9-0969141316670193","doi-asserted-by":"publisher","DOI":"10.1258\/jms.2012.012086"},{"key":"bibr10-0969141316670193","doi-asserted-by":"publisher","DOI":"10.2307\/2987937"},{"key":"bibr11-0969141316670193","doi-asserted-by":"publisher","DOI":"10.3310\/hta7110"}],"container-title":["Journal of Medical Screening"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0969141316670193","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0969141316670193","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0969141316670193","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T23:23:59Z","timestamp":1777677839000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0969141316670193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,21]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1177\/0969141316670193"],"URL":"https:\/\/doi.org\/10.1177\/0969141316670193","relation":{},"ISSN":["0969-1413","1475-5793"],"issn-type":[{"value":"0969-1413","type":"print"},{"value":"1475-5793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,21]]}}}