{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:28:13Z","timestamp":1790789293751,"version":"4.1.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tumour Biol."],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:p>Renal cell carcinoma, the most common neoplasm of adult kidney, accounts for about 3% of adult malignancies and is usually highly resistant to conventional therapy. MicroRNAs are a class of small non-coding RNAs, which have been previously shown to promote malignant initiation and progression. In this study, we focused our attention on miR-21, a well described oncomiR commonly upregulated in cancer. Using a cohort of 99 primary renal cell carcinoma samples, we showed that miR-21 expression in cancer tissues was higher than in adjacent non-tumor tissues whereas no significant difference was observed with stages, grades, and metastatic outcome. In vitro, miR-21 was also overexpressed in renal carcinoma cell lines compared to HK-2 human proximal tubule epithelial cell line. Moreover, using Boyden chambers and western blot techniques, we also showed that miR-21 overexpression increased migratory, invasive, proliferative, and anti-apoptotic signaling pathways whereas opposite results were observed using an anti-miR-21-based silencing strategy. Finally, we assessed the role of miR-21 in mediating renal cell carcinoma chemoresistance and further showed that miR-21 silencing significantly (1) increased chemosensitivity of paclitaxel, 5-fluorouracil, oxaliplatin, and dovitinib; (2) decreased expression of multi-drug resistance genes; and (4) increased SLC22A1\/OCT1, SLC22A2\/OCT2, and SLC31A1\/CTR1 platinum influx transporter expression. In conclusion, our results showed that miR-21 is a key actor of renal cancer progression and plays an important role in the resistance to chemotherapeutic drugs. In renal cell carcinoma, targeting miR-21 is a potential new therapeutic strategy to improve chemotherapy efficacy and consequently patient outcome.<\/jats:p>","DOI":"10.1177\/1010428317707372","type":"journal-article","created":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T11:44:30Z","timestamp":1500551070000},"page":"101042831770737","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":51,"title":["Targeting miR-21 decreases expression of multi-drug resistant genes and promotes chemosensitivity of renal carcinoma"],"prefix":"10.1177","volume":"39","author":[{"given":"Kelly","family":"Gaudelot","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Lille, Inserm, CHU Lille, UMR-S 1172, Team \u201cMucins, Epithelial Differentiation and Carcinogenesis,\u201d Jean-Pierre Aubert Research Center (JPARC), Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Baptiste","family":"Gibier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille, Inserm, CHU Lille, UMR-S 1172, Team \u201cMucins, Epithelial Differentiation and Carcinogenesis,\u201d Jean-Pierre Aubert Research Center (JPARC), Lille, France"},{"name":"CHU Lille, Pathology Institute, Centre de Biologie Pathologie, Rue Oscar Lambret, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Pottier","sequence":"additional","affiliation":[{"name":"EA4483, Universit\u00e9 de Lille, Facult\u00e9 de M\u00e9decine, P\u00f4le Recherche, Place de Verdun, Lille, France"},{"name":"CHU Lille, Department of Biochemistry and Molecular Biology, Centre de Biologie Pathologie, Rue Oscar Lambret, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"H\u00e9mon","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille, Inserm, CHU Lille, UMR-S 1172, Team \u201cMucins, Epithelial Differentiation and Carcinogenesis,\u201d Jean-Pierre Aubert Research Center (JPARC), Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Van Seuningen","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille, Inserm, CHU Lille, UMR-S 1172, Team \u201cMucins, Epithelial Differentiation and Carcinogenesis,\u201d Jean-Pierre Aubert Research Center (JPARC), Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Glowacki","sequence":"additional","affiliation":[{"name":"EA4483, Universit\u00e9 de Lille, Facult\u00e9 de M\u00e9decine, P\u00f4le Recherche, Place de Verdun, Lille, France"},{"name":"CHU Lille, Department of Nephrology, H\u00f4pital Huriez, Rue Michel Polonovski, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Leroy","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille, Inserm, CHU Lille, UMR-S 1172, Team \u201cMucins, Epithelial Differentiation and Carcinogenesis,\u201d Jean-Pierre Aubert Research Center (JPARC), Lille, France"},{"name":"CHU Lille, Pathology Institute, Centre de Biologie Pathologie, Rue Oscar Lambret, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Cauffiez","sequence":"additional","affiliation":[{"name":"EA4483, Universit\u00e9 de Lille, Facult\u00e9 de M\u00e9decine, P\u00f4le Recherche, Place de Verdun, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane","family":"Gnemmi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille, Inserm, CHU Lille, UMR-S 1172, Team \u201cMucins, Epithelial Differentiation and Carcinogenesis,\u201d Jean-Pierre Aubert Research Center (JPARC), Lille, France"},{"name":"CHU Lille, Pathology Institute, Centre de Biologie Pathologie, Rue Oscar Lambret, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Aubert","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille, Inserm, CHU Lille, UMR-S 1172, Team \u201cMucins, Epithelial Differentiation and Carcinogenesis,\u201d Jean-Pierre Aubert Research Center (JPARC), Lille, France"},{"name":"CHU Lille, Pathology Institute, Centre de Biologie Pathologie, Rue Oscar Lambret, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u00ebl","family":"Perrais","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille, Inserm, CHU Lille, UMR-S 1172, Team \u201cMucins, Epithelial Differentiation and Carcinogenesis,\u201d Jean-Pierre Aubert Research Center (JPARC), Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,7,17]]},"reference":[{"key":"bibr1-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.30314"},{"key":"bibr2-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.24713"},{"key":"bibr3-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2014.01.003"},{"key":"bibr4-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510565103"},{"key":"bibr5-1010428317707372","doi-asserted-by":"publisher","DOI":"10.3892\/br.2016.747"},{"key":"bibr6-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-014-0450-2"},{"key":"bibr7-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.626"},{"key":"bibr8-1010428317707372","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5495"},{"key":"bibr9-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103305"},{"key":"bibr10-1010428317707372","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.4814"},{"key":"bibr11-1010428317707372","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.1681"},{"key":"bibr12-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.12.029"},{"key":"bibr13-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"bibr14-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058014"},{"key":"bibr15-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-4905"},{"key":"bibr16-1010428317707372","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.1768"},{"key":"bibr17-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2012.02.003"},{"issue":"3","key":"bibr18-1010428317707372","first-page":"209","volume":"13","author":"Kowalczyk AE","year":"2016","journal-title":"Cancer Genomics Proteomics"},{"key":"bibr19-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1038\/srep10272"},{"key":"bibr20-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1038\/nature12222"},{"key":"bibr21-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.11.064"},{"key":"bibr22-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-014-1976-8"},{"issue":"8","key":"bibr23-1010428317707372","first-page":"2060","volume":"7","author":"Lei B-X","year":"2014","journal-title":"Int J Clin Exp Med"},{"key":"bibr24-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds496"},{"key":"bibr25-1010428317707372","first-page":"3203","volume":"9","author":"Passadouro M","year":"2014","journal-title":"Int J Nanomedicine"},{"key":"bibr26-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24080"},{"key":"bibr27-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.23499.x"},{"key":"bibr28-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2013.08.008"},{"key":"bibr29-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2015.02.002"},{"key":"bibr30-1010428317707372","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.4568"},{"key":"bibr31-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3923-z"},{"key":"bibr32-1010428317707372","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11888"},{"key":"bibr33-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.02.034"},{"key":"bibr34-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-014-2984-8"},{"key":"bibr35-1010428317707372","volume":"3","author":"Li Q","year":"2013","journal-title":"Sci Rep"},{"key":"bibr36-1010428317707372","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2016.03.008"}],"container-title":["Tumor Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1010428317707372","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1010428317707372","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1010428317707372","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T23:24:49Z","timestamp":1777677889000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.sagepub.com\/doi\/10.1177\/1010428317707372"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":36,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1177\/1010428317707372"],"URL":"https:\/\/doi.org\/10.1177\/1010428317707372","relation":{},"ISSN":["1010-4283","1423-0380"],"issn-type":[{"value":"1010-4283","type":"print"},{"value":"1423-0380","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7]]}}}