{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:11:52Z","timestamp":1777929112679,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tumour Biol."],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:p>Human papillomavirus is detected in over 50% of oropharyngeal squamous cell carcinomas. Human papillomavirus\u2013positive oropharyngeal squamous cell carcinomas differ from human papillomavirus\u2013negative tumors, and both expression patterns are classified as distinct entities. The Bmi-1 oncogene is a well-known member of the mammalian polycomb-group family. HESC5:3 and HES77 are newly developed monoclonal antibodies produced against undifferentiated embryonic stem cells. Our aim was to explore their roles in both human papillomavirus\u2013positive and \u2013negative oropharyngeal squamous cell carcinomas. Our cohort comprised 202 consecutive oropharyngeal squamous cell carcinoma patients diagnosed and treated with curative intent. We used tissue microarray tumor blocks to study the immunohistochemical expression of Bmi-1, HESC5:3, and HES77. We compared the expressions of these stem cell markers with p16 immunoexpression and human papillomavirus status, as well as with other characteristics of the tumor, and with patients\u2019 clinical data and follow-up data. Human papillomavirus\u2013 and p16-positive tumors expressed less Bmi-1 and more HESC5:3 than the negative tumors. HES77 expression was high in human papillomavirus\u2013positive oropharyngeal squamous cell carcinoma, but it did not correlate with p16 positivity. In our multivariable model, Bmi-1 and HESC5:3 were still associated with human papillomavirus, but the association between human papillomavirus and HES77 remained absent. In conclusion, Bmi-1, HESC5:3, and HES77 may have a different role in human papillomavirus\u2013positive and human papillomavirus\u2013negative tumors. There was no correlation between Bmi-1, HESC5:3, and HES77 expression and survival.<\/jats:p>","DOI":"10.1177\/1010428319840473","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T16:54:51Z","timestamp":1553705691000},"page":"101042831984047","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["The expression and prognostic value of stem cell markers Bmi-1, HESC5:3, and HES77 in human papillomavirus\u2013positive and \u2013negative oropharyngeal squamous cell carcinoma"],"prefix":"10.1177","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9044-366X","authenticated-orcid":false,"given":"Hesham","family":"Mohamed","sequence":"first","affiliation":[{"name":"Department of Pathology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland"},{"name":"Department of Histology, Omar Al-Mukhtar University, El-Beida, Libya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaana","family":"Hagstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Pathology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland"},{"name":"Translational Cancer Medicine Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauri","family":"Jouhi","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2014Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timo","family":"Atula","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2014Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alhadi","family":"Almangush","sequence":"additional","affiliation":[{"name":"Department of Pathology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antti","family":"M\u00e4kitie","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2014Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland"},{"name":"Division of Ear, Nose and Throat Diseases, Department of Clinical Sciences, Intervention and Technology and Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden"},{"name":"Research Programme in Systems Oncology, Faculty of Medicine, University of Helsinki, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caj","family":"Haglund","sequence":"additional","affiliation":[{"name":"Translational Cancer Medicine Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland"},{"name":"Department of Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,3,27]]},"reference":[{"key":"bibr1-1010428319840473","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990315)80:6<827::AID-IJC6>3.0.CO;2-P"},{"issue":"11","key":"bibr2-1010428319840473","first-page":"3282","volume":"48","author":"Blot WJ","year":"1988","journal-title":"Cancer Res"},{"key":"bibr3-1010428319840473","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa065497"},{"key":"bibr4-1010428319840473","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-16-0633"},{"key":"bibr5-1010428319840473","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2016.02.011"},{"key":"bibr6-1010428319840473","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-010-1037-y"},{"key":"bibr7-1010428319840473","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24339"},{"key":"bibr8-1010428319840473","unstructured":"El-Naggar AK, Chan JKC, Grandis JR, et al. 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