{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:48:39Z","timestamp":1777931319009,"version":"3.51.4"},"reference-count":17,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p>Procedurally similar competitive enzyme-linked immunoassay (cELISA) methods were developed for the serodiagnosis of Babesia equi and Babesia caballi (piroplasmosis), Trypanosoma equiperdum (dourine), and Burkholderia mallei (glanders) infections in horses. Apparent test specificities for the B. equi, B. caballi, T. equiperdum, and B. mallei cELISAs were 99.2%, 99.5%, 98.9%, and 98.9%, respectively. Concordances and kappa values between the complement fixation (CF) and the cELISA procedures for the serodiagnosis of B. equi, B. caballi, T. equiperdum, and B. mallei infections in experimentally exposed horses were 76% and 0.55, 89% and 0.78, 97% and 0.95, and 70% and 0.44, respectively. The cELISA method may be a technically more reproducible, objective, and convenient approach for piroplasmosis, dourine, and glanders serodiagnosis in qualifying animals for international movement and disease eradication programs than the CF systems currently in use. Use of the cELISA method also obviated the problems associated with testing hemolyzed or anticomplementary sera.<\/jats:p>","DOI":"10.1177\/104063870001200108","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:20:37Z","timestamp":1305217237000},"page":"46-50","source":"Crossref","is-referenced-by-count":32,"title":["Procedurally Similar Competitive Immunoassay Systems for the Serodiagnosis of\n                    <i>Babesia Equi, Babesia Caballi, Trypanosoma Equiperdum<\/i>\n                    , and\n                    <i>Burkholderia Mallei<\/i>\n                    Infection in Horses"],"prefix":"10.1177","volume":"12","author":[{"given":"Jonathan","family":"Katz","sequence":"first","affiliation":[{"name":"Diagnostic Bacteriology Laboratory, National Veterinary Services Laboratories, US Department of Agriculture, Animal and Health Inspection Services, Ames, IA 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Dewald","sequence":"additional","affiliation":[{"name":"Diagnostic Bacteriology Laboratory, National Veterinary Services Laboratories, US Department of Agriculture, Animal and Health Inspection Services, Ames, IA 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judy","family":"Nicholson","sequence":"additional","affiliation":[{"name":"Diagnostic Bacteriology Laboratory, National Veterinary Services Laboratories, US Department of Agriculture, Animal and Health Inspection Services, Ames, IA 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2000,1,1]]},"reference":[{"key":"bibr1-104063870001200108","first-page":"91","volume-title":"Foreign animal diseases","author":"Anonymous","year":"1992"},{"key":"bibr2-104063870001200108","volume-title":"Piroplasmosis, dourine, and glanders complement fixation test\u2014micro method.","author":"Anonymous","year":"1992"},{"key":"bibr3-104063870001200108","first-page":"394","volume-title":"Manual of standards for diagnostic tests and vaccines","author":"Anonymous","year":"1996","edition":"3"},{"key":"bibr4-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(95)80152-9"},{"key":"bibr5-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(96)01074-6"},{"key":"bibr6-104063870001200108","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.2460\/ajvr.1993.54.08.1201","volume":"54","author":"Hagebock JM","year":"1993","journal-title":"Am J Vet Res"},{"key":"bibr7-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1177\/104063879300500121"},{"key":"bibr8-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.7.2285-2290.1999"},{"key":"bibr9-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1177\/104063879901100316"},{"key":"bibr10-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(96)80066-2"},{"key":"bibr11-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.29.9.2056-2058.1991"},{"key":"bibr12-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(88)90088-X"},{"key":"bibr13-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1995.tb00201.x"},{"key":"bibr14-104063870001200108","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook JE","year":"1989","edition":"2"},{"key":"bibr15-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(97)00228-8"},{"key":"bibr16-104063870001200108","volume-title":"Statistical methods","author":"Snedecor GW","year":"1967","edition":"6"},{"key":"bibr17-104063870001200108","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(86)90092-0"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870001200108","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870001200108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:43:12Z","timestamp":1777682592000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063870001200108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["10.1177\/104063870001200108"],"URL":"https:\/\/doi.org\/10.1177\/104063870001200108","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,1]]}}}