{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:49:51Z","timestamp":1777931391938,"version":"3.51.4"},"reference-count":18,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2001,9,1]],"date-time":"2001-09-01T00:00:00Z","timestamp":999302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:p>Serologic responses to the newly introduced rough Brucella abortus vaccine strain RB51 have been determined in a dot-blot format using \u03b3-irradiated RB51 cells as the antigen. Because \u03b3-irradiated cells are not easily prepared and the signal from cells was not always reliable, an alternative antigen was sought. Detergent extracts of B. abortus RB51 were prepared using zwittergent 3\u201314, Triton X-100, and sodium dodecyl sulfate (SDS) and examined in a dot-blot format. Zwittergent 3\u201314 extracts and \u03b3-irradiated RB51 cells gave the same titers. Unlike \u03b3-irradiated RB51 cells, zwittergent 3\u201314 extracts produced signals consistently, and the signals were easily interpreted. Triton X-100 extracts interfered with signal development, and SDS extracts resulted in a high background signal. Western blot analyses revealed several outer membrane proteins in the zwittergent 3\u201314 extract. The major antigens in the extract had apparent molecular weights of &lt;20,000.<\/jats:p>","DOI":"10.1177\/104063870101300507","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:20:37Z","timestamp":1305217237000},"page":"408-412","source":"Crossref","is-referenced-by-count":3,"title":["Use of Detergent Extracts of\n                    <i>Brucella Abortus<\/i>\n                    RB51 to Detect Serologic Responses in RB51-Vaccinated Cattle"],"prefix":"10.1177","volume":"13","author":[{"given":"Shirley M.","family":"Hailing","sequence":"first","affiliation":[{"name":"Bacterial Diseases of Livestock Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA 50010-0070"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy A.","family":"Koster","sequence":"additional","affiliation":[{"name":"Bacterial Diseases of Livestock Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA 50010-0070"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2001,9,1]]},"reference":[{"key":"bibr1-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.6.6.787-790.1999"},{"key":"bibr2-104063870101300507","first-page":"63","volume-title":"Techniques for the brucellosis laboratory","author":"Alton GG","year":"1988"},{"key":"bibr3-104063870101300507","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.2460\/ajvr.1993.54.10.1591","volume":"54","author":"Cheville NF","year":"1993","journal-title":"Am J Vet Res"},{"key":"bibr4-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.58.12.3980-3987.1990"},{"key":"bibr5-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08547.x"},{"key":"bibr6-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-138-7-1543"},{"key":"bibr7-104063870101300507","first-page":"55","volume":"23","author":"Ebani VV","year":"2000","journal-title":"Microbiologica"},{"key":"bibr8-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.12.6637-6640.1990"},{"key":"bibr9-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(95)00121-2"},{"key":"bibr10-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"bibr11-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1177\/104063879700900404"},{"key":"bibr12-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.46.1.188-194.1984"},{"key":"bibr13-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(91)90091-S"},{"key":"bibr14-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.32.4.1065-1066.1994"},{"key":"bibr15-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(94)05311-F"},{"key":"bibr16-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4960-4962.1999"},{"key":"bibr17-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.1.100-107.1996"},{"key":"bibr18-104063870101300507","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.9.3633-3639.1994"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870101300507","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870101300507","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:45:49Z","timestamp":1777682749000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063870101300507"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,9]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2001,9]]}},"alternative-id":["10.1177\/104063870101300507"],"URL":"https:\/\/doi.org\/10.1177\/104063870101300507","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,9]]}}}