{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:42:52Z","timestamp":1792712572132,"version":"4.5.0"},"reference-count":18,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:p>\n                    A new monoclonal antibody (MAb), F99\/97.6.1, that has been used to demonstrate scrapie-associated prion protein PrP\n                    <jats:sup>sc<\/jats:sup>\n                    in brain and lymphoid tissues of domestic sheep with scrapie was used in an immunohistochemistry assay for diagnosis of chronic wasting disease (CWD) in mule deer ( Odocoileus hemionus). The MAb F99\/97.6.1 immunohistochemistry assay was evaluated in brain and tonsil tissue from 100 mule deer that had spongiform encephalopathy compatible with CWD and from 1,050 mule deer outside the CWD-endemic area. This MAb demonstrated abnormal protease-resistant prion protein (PrP\n                    <jats:sup>res<\/jats:sup>\n                    ) in brains of all of the 100 mule deer and in 99 of the 100 tonsil samples. No immunostaining was seen in samples collected from deer outside the endemic area. MAb F99\/97.6.1 demonstrated excellent properties for detection of PrP\n                    <jats:sup>res<\/jats:sup>\n                    in fresh, frozen, or mildly to moderately autolytic samples of brain and tonsil. This immunohistochemistry assay is a sensitive, specific, readily standardized diagnostic test for CWD in deer.\n                  <\/jats:p>","DOI":"10.1177\/104063870201400102","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:20:37Z","timestamp":1305217237000},"page":"3-7","source":"Crossref","is-referenced-by-count":94,"title":["Validation of Monoclonal Antibody F99\/97.6.1 for Immunohistochemical Staining of Brain and Tonsil in Mule Deer (\n                    <i>Odocoileus Hemionus<\/i>\n                    ) with Chronic Wasting Disease"],"prefix":"10.1177","volume":"14","author":[{"given":"T. R.","family":"Spraker","sequence":"first","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine, Colorado State University, Fort Collins, CO 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. I.","family":"O'Rourke","sequence":"additional","affiliation":[{"name":"Animal Disease Research Unit, ARS\/ USDA, 3003 ADBF, Pullman, WA 99164"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Balachandran","sequence":"additional","affiliation":[{"name":"Animal Disease Research Inspection, CFIA, 3851 Fallowfield Road, Nepean, Ontario K2G 5HP, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. R.","family":"Zink","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine, Colorado State University, Fort Collins, CO 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. A.","family":"Cummings","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine, Colorado State University, Fort Collins, CO 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. W.","family":"Miller","sequence":"additional","affiliation":[{"name":"Colorado Division of Wildlife, 317 West Prospect Road, Fort Collins, CO 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. E.","family":"Powers","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine, Colorado State University, Fort Collins, CO 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2002,1,1]]},"reference":[{"key":"bibr1-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.2.375"},{"key":"bibr2-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(91)90552-5"},{"key":"bibr3-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1007\/BF00310125"},{"key":"bibr4-104063870201400102","first-page":"3","volume-title":"Slow transmissible diseases of the nervous system","author":"Hadlow WJ","year":"1979"},{"key":"bibr5-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1007\/BF00386258"},{"key":"bibr6-104063870201400102","first-page":"230","volume":"57","author":"Kitamoto T","year":"1987","journal-title":"Lab Invest"},{"key":"bibr7-104063870201400102","volume-title":"Histopathologic methods and color atlas of special stains and tissues artifacts","author":"Luna LG","year":"1992"},{"key":"bibr8-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1177\/104063879400600315"},{"key":"bibr9-104063870201400102","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-36.4.676"},{"key":"bibr10-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.9.3254-3259.2000"},{"key":"bibr11-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.6.1750-1755.1998"},{"key":"bibr12-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1177\/104063870001200618"},{"key":"bibr13-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-10-2757"},{"key":"bibr14-104063870201400102","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-33.1.1"},{"key":"bibr15-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.5.1228-1231.1996"},{"key":"bibr16-104063870201400102","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-16.1.89"},{"key":"bibr17-104063870201400102","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-18.4.465"},{"key":"bibr18-104063870201400102","doi-asserted-by":"publisher","DOI":"10.1177\/030098589303000105"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870201400102","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870201400102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:46:14Z","timestamp":1777682774000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063870201400102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1177\/104063870201400102"],"URL":"https:\/\/doi.org\/10.1177\/104063870201400102","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1]]}}}