{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:07:36Z","timestamp":1789873656219,"version":"4.0.1"},"reference-count":12,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:p>Sequence analysis of the 5.8S rRNA gene and the internal transcribed spacer regions (ITSRs) was used to compare trichomonadid protozoa ( n = 39) of varying morphologies isolated from the bovine preputial cavity. A multiple sequence alignment was performed with bovine isolate sequences and other trichomonadid protozoa sequences available in GenBank. As a group, Tritrichomonas foetus isolates ( n = 7) had nearly complete homology. A similarity matrix showed low homology between the T. foetus isolates and other trichomonads recovered from cattle (&lt;70%). Two clusters of trichomonads other than T. foetus were identified. Eighteen isolates comprised 1 group. These isolates shared &gt;99% homology among themselves and with Pen-tatrichomonas hominis. The other non\u2013 T. foetus cluster ( n = 14) did not exhibit a high degree of homology (&lt;87%) with other bovine isolates or any of the trichomonad sequences available in GenBank. The sequence homology among isolates in that cluster was &gt;99%, except for 1 isolate that varied from the others in both ITSRs (\u223c2% dissimilarity). Sequence analysis of the 5.8S rRNA gene and ITSRs was useful for comparing trichomonadid protozoa isolated from the bovine preputial cavity and demonstrated that 2 distinct types of trichomonads constituted the non\u2013 T. foetus isolates recovered from the bovine preputial cavity.<\/jats:p>","DOI":"10.1177\/104063870301500104","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:20:37Z","timestamp":1305217237000},"page":"14-20","source":"Crossref","is-referenced-by-count":46,"title":["Comparison of the 5.8S rRNA Gene and Internal Transcribed Spacer Regions of Trichomonadid Protozoa Recovered from the Bovine Preputial Cavity"],"prefix":"10.1177","volume":"15","author":[{"given":"R. L.","family":"Walker","sequence":"first","affiliation":[{"name":"California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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