{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:23:46Z","timestamp":1791174226924,"version":"4.1.0"},"reference-count":13,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:p>Serologic diagnosis of ovine contagious agalactia ( Mycoplasma agalactiae) with the enzyme-linked immunosorbent assay (ELISA) developed by Agence Fran\u00e7aise de S\u00e9ceurit\u00e9 Sanitaire des Aliments (AFS-SA) may produce a few false-positive (FP) and false-negative (FN) results. When the prevalence of disease is low, these erroneous results may generate problems for eradication schemes. To prevent this, 2 commercial ELISAs were compared with the AFSSA ELISA. Flocks of known status were selected and classified into 4 categories: true positive (TP), FP, true negative (TN), and FN; 20 sheep per flock were submitted for blood sampling. A flock was considered positive when at least 1 out of 20 sera was positive or 2 sera were doubtful. In the flock, the diagnostic sensitivity of the 3 kits was very good (100%), and the diagnostic specificity showed an improvement from 46% (AFSSA test) to 88% and 92% (commercial tests). Considering individual animals, very few positive ewes were detected within TN or FP flocks; the proportion of positive ewes varied greatly from one kit to another (48% to 82%) within TP flocks. The kinetics of antibody response in sheep experimentally infected with various field strains of M. agalactiae were quite similar with all 3 ELISAs. The agreement between the 3 tests, assessed using the kappa value, varied from moderate to good (respective values of 0.56, 0.61, and 0.86). The 2 commercial ELISAs showed better performances, probably because of a superior analytical sensitivity, and are a good alternative for the serodiagnosis of contagious agalactia in sheep.<\/jats:p>","DOI":"10.1177\/104063870301500311","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:20:37Z","timestamp":1305217237000},"page":"281-285","source":"Crossref","is-referenced-by-count":11,"title":["Comparison of Three Enzyme-Linked Immunosorbent Assays for Serologic Diagnosis of Contagious Agalactia in Sheep"],"prefix":"10.1177","volume":"15","author":[{"given":"Michel","family":"P\u00e9pin","sequence":"first","affiliation":[{"name":"Agence Fran\u00e7aise de S\u00e9ceurit\u00e9 Sanitaire des Aliments (AFSSA), F-06902 Sophia Antipolis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Dufour","sequence":"additional","affiliation":[{"name":"Agence Fran\u00e7aise de S\u00e9ceurit\u00e9 Sanitaire des Aliments (AFSSA), F-06902 Sophia Antipolis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurice","family":"Lambert","sequence":"additional","affiliation":[{"name":"Agence Fran\u00e7aise de S\u00e9ceurit\u00e9 Sanitaire des Aliments (AFSSA), F-06902 Sophia Antipolis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Aubert","sequence":"additional","affiliation":[{"name":"Agence Fran\u00e7aise de S\u00e9ceurit\u00e9 Sanitaire des Aliments (AFSSA), F-06902 Sophia Antipolis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e8le","family":"Valognes","sequence":"additional","affiliation":[{"name":"Laboratoire D\u00e9partemental d'Analyses, F-64010 Pau, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Rotis","sequence":"additional","affiliation":[{"name":"ADMA, F-64000 Pau, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Van de Wiele","sequence":"additional","affiliation":[{"name":"ADMA, F-64000 Pau, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Bergonier","sequence":"additional","affiliation":[{"name":"National Veterinary School, INRA-UMR 959, F-31076 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2003,5,1]]},"reference":[{"key":"bibr1-104063870301500311","doi-asserted-by":"publisher","DOI":"10.20506\/rst.16.3.1062"},{"key":"bibr2-104063870301500311","doi-asserted-by":"publisher","DOI":"10.1177\/104063879200400126"},{"key":"bibr3-104063870301500311","first-page":"15","volume-title":"Manual of standards for diagnostic tests and vaccines","author":"Jacobson R","year":"2000","edition":"4"},{"key":"bibr4-104063870301500311","first-page":"303","volume":"136","author":"Lambert M","year":"1985","journal-title":"Rev Med Vet"},{"key":"bibr5-104063870301500311","first-page":"699","volume":"6","author":"Lambert M","year":"1987","journal-title":"Rev Sci Tech Off Int Epizoot"},{"key":"bibr6-104063870301500311","doi-asserted-by":"publisher","DOI":"10.1177\/104063879801000403"},{"key":"bibr7-104063870301500311","first-page":"97","volume-title":"COST 826: First Workshop: agriculture and biotechnology. Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics","author":"Lambert M","year":"1996"},{"key":"bibr8-104063870301500311","first-page":"119","volume-title":"COST 826: 3rd Workshop: mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics","author":"P\u00e9pin M","year":"1998"},{"key":"bibr9-104063870301500311","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.6213-6216.1999"},{"key":"bibr10-104063870301500311","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00164-9"},{"key":"bibr11-104063870301500311","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2000104"},{"key":"bibr12-104063870301500311","doi-asserted-by":"publisher","DOI":"10.1016\/S0921-4488(96)00996-0"},{"key":"bibr13-104063870301500311","first-page":"266","volume-title":"Veterinary epidemiology","author":"Thrusfield M","year":"1995","edition":"2"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870301500311","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870301500311","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:08:58Z","timestamp":1777680538000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063870301500311"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,5]]}},"alternative-id":["10.1177\/104063870301500311"],"URL":"https:\/\/doi.org\/10.1177\/104063870301500311","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,5]]}}}