{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:31:08Z","timestamp":1777930268206,"version":"3.51.4"},"reference-count":11,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:p>\n                    A monoclonal antibody dot-blot assay was used to evaluate detergent lysates of tonsil tissue from mule deer to detect PrP\n                    <jats:sup>CWD<\/jats:sup>\n                    , the marker for the cervid transmissible spongiform encephalopathy chronic wasting disease (CWD). Samples of formalin-fixed brain and tonsil tissues from mule deer were examined for PrP\n                    <jats:sup>CWD<\/jats:sup>\n                    using immunohistochemistry (IHC) with Mab F99\/97.6.1, the gold standard for diagnosis of preclinical CWD. The contralateral tonsil from each of the 143 deer was prepared for confirmatory IHC and as a 10% (wt\/vol) detergent lysate without purification or enrichment steps for monoclonal antibody dot-blot assay. PrP\n                    <jats:sup>CWD<\/jats:sup>\n                    was detected by dot-blot assay in 49 of 50 samples considered positive by IHC. Forty-eight of the positive samples were evaluated with a quantitative dot-blot assay calibrated with recombinant PrP. Tonsillar PrP\n                    <jats:sup>CWD<\/jats:sup>\n                    concentrations ranged from 34 to 1,188 ng per 0.5 mg starting wet weight of tissue. The abundant PrP\n                    <jats:sup>CWD<\/jats:sup>\n                    in mule deer tonsil will facilitate development and validation of high-throughput screening tests for CWD in large populations of free-ranging deer.\n                  <\/jats:p>","DOI":"10.1177\/104063870301500403","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:20:37Z","timestamp":1305217237000},"page":"320-323","source":"Crossref","is-referenced-by-count":19,"title":["Abundant PrP\n                    <sup>CWD<\/sup>\n                    in Tonsil from Mule Deer with Preclinical Chronic Wasting Disease"],"prefix":"10.1177","volume":"15","author":[{"given":"Katherine I.","family":"O'Rourke","sequence":"first","affiliation":[{"name":"Agricultural Research Service, U.S. Department of Agriculture, Animal Disease Research Unit, Pullman, WA 99164"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongyue","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Washington State University College of Veterinary Medicine, Pullman, WA 99164"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Lyda","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, U.S. Department of Agriculture, Animal Disease Research Unit, Pullman, WA 99164"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Gomez","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, U.S. Department of Agriculture, Animal Disease Research Unit, Pullman, WA 99164"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth S.","family":"Williams","sequence":"additional","affiliation":[{"name":"Wyoming State Veterinary Laboratory, Laramie, WY 82070"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenbin","family":"Tuo","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, U.S. Department of Agriculture, Animal Disease Research Unit, Pullman, WA 99164"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael W.","family":"Miller","sequence":"additional","affiliation":[{"name":"Colorado Division of Wildlife, Fort Collins, CO 80526"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2003,7,1]]},"reference":[{"key":"bibr1-104063870301500403","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00421-9"},{"key":"bibr2-104063870301500403","doi-asserted-by":"publisher","DOI":"10.1136\/vr.149.19.577"},{"key":"bibr3-104063870301500403","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(91)90552-5"},{"key":"bibr4-104063870301500403","doi-asserted-by":"publisher","DOI":"10.1136\/vr.151.20.610"},{"key":"bibr5-104063870301500403","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.9.3254-3259.2000"},{"key":"bibr6-104063870301500403","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.6.1750-1755.1998"},{"key":"bibr7-104063870301500403","doi-asserted-by":"publisher","DOI":"10.1007\/s004010051106"},{"key":"bibr8-104063870301500403","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-10-2757"},{"key":"bibr9-104063870301500403","doi-asserted-by":"publisher","DOI":"10.1177\/104063870201400102"},{"key":"bibr10-104063870301500403","doi-asserted-by":"publisher","DOI":"10.20506\/rst.21.2.1340"},{"key":"bibr11-104063870301500403","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-16.1.89"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870301500403","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870301500403","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:09:01Z","timestamp":1777680541000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063870301500403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":11,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1177\/104063870301500403"],"URL":"https:\/\/doi.org\/10.1177\/104063870301500403","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]}}}