{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:31:26Z","timestamp":1777930286060,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:p>\n                    In the present study, laboratory techniques were used to diagnose canine G\n                    <jats:sub>M2<\/jats:sub>\n                    -gangliosidosis using blood and cerebrospinal fluid (CSF) that can be collected noninvasively from living individuals. Lysosomal acid \u03b2-hexosaminidase (Hex) was measured spectrofluorometrically using 4-methylumbelliferyl N-acetyl-\u03b2-d-glucosaminide and 4-methylumbelliferyl 7-(6-sulfo-2-acetamido-2-deoxy-\u03b2-d-glucopyranoside) as substrates. Main isoenzymes A and B of Hex in leukocytes were also analyzed using cellulose acetate membrane electrophoresis. G\n                    <jats:sub>M2<\/jats:sub>\n                    -ganglioside in CSF was detected and determined quantitatively by using thin-layer chromatography\/enzyme-immunostaining method with anti-G\n                    <jats:sub>M2<\/jats:sub>\n                    -ganglioside antibody. In normal dogs, Hex activities could be determined in leukocytes, serum, and CSF, and the total activities were markedly reduced in all the enzyme sources in a dog with Sandhoff disease. Electrophoresis of a leukocyte lysate from a normal dog showed that the Hex A and Hex B were not separated distinctively with formation of a broad band, whereas there were no bands in electrophoresis of a lysate from a dog with Sandhoff disease, showing a deficiency in the total enzyme activity. G\n                    <jats:sub>M2<\/jats:sub>\n                    -ganglioside could be detected and determined quantitatively in as little as 100 \u03bcl of canine CSF. G\n                    <jats:sub>M2<\/jats:sub>\n                    -ganglioside in CSF in a dog with Sandhoff disease increased to 46 times the normal level. In conclusion, the methods in the present study are useful for diagnosis of canine G\n                    <jats:sub>M2<\/jats:sub>\n                    -gangliosidosis. These techniques enable definitive and early diagnosis of canine G\n                    <jats:sub>M2<\/jats:sub>\n                    -gangliosidosis even if tissues and organs cannot be obtained.\n                  <\/jats:p>","DOI":"10.1177\/104063870401600107","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:20:37Z","timestamp":1305217237000},"page":"39-44","source":"Crossref","is-referenced-by-count":14,"title":["Laboratory Diagnosis of Canine G\n                    <sub>M2<\/sub>\n                    -Gangliosidosis using Blood and Cerebrospinal Fluid"],"prefix":"10.1177","volume":"16","author":[{"given":"Osamu","family":"Yamato","sequence":"first","affiliation":[{"name":"Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060\u20130818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Satoh","sequence":"additional","affiliation":[{"name":"Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060\u20130818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoaki","family":"Matsuki","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Pathobiology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Tokyo 113\u20138657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichiro","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Pathobiology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Tokyo 113\u20138657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060\u20130818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimitsu","family":"Maede","sequence":"additional","affiliation":[{"name":"Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060\u20130818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2004,1,1]]},"reference":[{"key":"bibr1-104063870401600107","first-page":"615","volume-title":"Consultations in feline internal medicine","author":"Baker 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